ABSTRACT
It is presented that the newly synthesized mono- and disubstituted 2,5-dimercapto-1,3,4-thiodiazole derivatives exert immunotropic activity. This effect was evaluated by measuring plaque forming cell number, circulating immunoglobulins level, autologous rosette number, mitogenic activity and by performing popliteal lymph node assay. It is shown that intensity of immunotropic activity depends on chemical structure: mono-substituted derivatives had a strong suppressive effect, disubstituted compounds showed the variable activity. One compound of this group exerted a stimulatory effect. The tested compounds were active in popliteal lymph node assay and had no mitogenic activity.
Subject(s)
Immunosuppressive Agents/chemical synthesis , Animals , Carbamates/chemical synthesis , Female , Immunosuppressive Agents/pharmacology , Lethal Dose 50 , Lymphocyte Activation/drug effects , Male , Mice , Mice, Inbred BALB C , Rosette Formation , Structure-Activity RelationshipABSTRACT
The effect of an organophosphorus pesticide-phosalone on some parameters of humoral and cellular immunologic response in mice was investigated. Haematological and morphological examinations were made parallelly. It was found that immunotropic effect of phosalone was a reversible process.
Subject(s)
Graft vs Host Reaction/immunology , Hypersensitivity, Delayed , Insecticides/toxicity , Macrophages, Peritoneal/immunology , Organothiophosphorus Compounds/toxicity , Adrenal Glands/drug effects , Adrenal Glands/pathology , Animals , Antibody Formation/drug effects , Body Weight/drug effects , Female , Immunity, Cellular/drug effects , Interleukin-1/biosynthesis , Lethal Dose 50 , Leukocyte Count/drug effects , Lymph Nodes/drug effects , Lymph Nodes/pathology , Lymphocyte Transfusion , Macrophages, Peritoneal/drug effects , Male , Mice , Mice, Inbred C57BL , Mice, Inbred DBA , Organ Size/drug effects , Rosette Formation , Spleen/drug effects , Spleen/pathology , Thymus Gland/drug effects , Thymus Gland/pathologyABSTRACT
The effect of organophosphate pesticide--Chlorfenwinfos on the selected parameters of humoral and cellular immunologic response in mice was investigated. Hematologic and histologic examinations were run parallelly. The animals were treated with pesticide p.o. during 90 days in doses 1/100, 1/50 and 1/25 of LD50. It was found that Chlorfenwinfos exerts immunotropic effect. After high doses the strong suppressive effect in PFC and EA rosettes was observed. Significant involution of thymus was noted. Il-1 activity, DTH reaction (24 h after challenge) and endogenous and exogenous spleen colonies were stimulated. It seems that immunotropic effect of this pesticide is reversible. Three weeks after the exposure to this compound had been stopped, PFC, IgG and IgM level came back to the normal value.
Subject(s)
Chlorfenvinphos/toxicity , Immune System/drug effects , Animals , Antibody-Producing Cells/drug effects , Colony-Forming Units Assay , Female , Graft vs Host Reaction/drug effects , Immunity, Cellular , Immunoglobulin G/blood , Immunoglobulin M/blood , Interleukin-1/metabolism , Lethal Dose 50 , Leukocyte Count , Lymphoid Tissue/drug effects , Male , Mice , Mice, Inbred C57BL , Mice, Inbred DBA , Rosette Formation , Spleen/transplantation , T-Lymphocytes, Regulatory/drug effectsABSTRACT
The effect of 2-isopropoxyphenyl methylcarbamate (Unden) (10- or 30-day exposure) on the selected parameters of humoral and cellular immunological response in mice was investigated. Hematologic and histologic examinations were run parallelly. It was noted that this compound exerts immunotropic effect. Immunosuppressive effect was prevailing (PFC, GvH, humoral response to SRBC) but in some tests also stimulatory effect was observed (number of B cells, Il-1, stimulation and proliferation of reticular cells were also observed in the spleen and lymphatic nodes). Immunotropic effect of Unden was reversible - after the exposure to pesticide had been stopped, the investigated parameters of the immunological system activity (PFC, IgG, IgM) spontaneously came back to the norm.
Subject(s)
Immune System/drug effects , Propoxur/toxicity , Animals , Antibody-Producing Cells/drug effects , Female , Graft vs Host Reaction/drug effects , Immunity, Cellular/drug effects , Immunoglobulins/metabolism , Interleukin-1/metabolism , Lethal Dose 50 , Lymphocyte Subsets/drug effects , Lymphoid Tissue/drug effects , Lymphoid Tissue/pathology , Male , Mice , Mice, Inbred C57BLABSTRACT
The effect of deltamethrin (10- or 30-day exposure) on selected parameters of humoral and cellular immune response in mice was studied. In parallel also hematologic and histologic examinations were performed. It was found that deltamethrin exerts negligible immunotropic effect. The prevailing was suppressive effect, and in some cases also stimulatory effect (PFC, GvH, Il-1, exogenous CFU-s). This negligible activity on the immunological system may be attributed to low toxicity of deltamehtrin in relation to the cells of immunological system or to too short exposure of the animals to its effect.
