Conducting Polymers for the Design of Tactile Sensors.

This paper provides an overview of the application of conducting polymers (CPs) used in the design of tactile sensors. While conducting polymers can be used as a base in a variety of forms, such as films, particles, matrices, and fillers, the CPs generally remain the same. This paper, first, discusses the chemical and physical properties of conducting polymers. Next, it discusses how these polymers might be involved in the conversion of mechanical effects (such as pressure, force, tension, mass, displacement, deformation, torque, crack, creep, and others) into a change in electrical resistance through a charge transfer mechanism for tactile sensing. Polypyrrole, polyaniline, poly(3,4-ethylenedioxythiophene), polydimethylsiloxane, and polyacetylene, as well as application examples of conducting polymers in tactile sensors, are overviewed. Attention is paid to the additives used in tactile sensor development, together with conducting polymers. There is a long list of additives and composites, used for different purposes, namely: cotton, polyurethane, PDMS, fabric, Ecoflex, Velostat, MXenes, and different forms of carbon such as graphene, MWCNT, etc. Some design aspects of the tactile sensor are highlighted. The charge transfer and operation principles of tactile sensors are discussed. Finally, some methods which have been applied for the design of sensors based on conductive polymers, are reviewed and discussed.

The application of DNA polymerases and Cas9 as representative of DNA-modifying enzymes group in DNA sensor design (review).

Rapid detection of nucleic acids (DNA or RNA) by inexpensive, selective, accurate, and highly sensitive methods is very important for biosensors. DNA-sensors based on DNA-modifying enzymes for fast determination and monitoring of pathogenic (Zika, Dengue, SARS-Cov-2 (inducer of COVID-19), human papillomavirus, HIV, etc.) viruses and diagnosis of virus-induced diseases is a key factor of this overview. Recently, DNA-modifying enzymes (Taq DNA polymerase, Phi29 DNA polymerase) have been widely used for the diagnosis of virus or pathogenic disease by gold standard (PCR, qPCR, RT-qPCR) methods, therefore, alternative methods have been reviewed. The main mechanisms of DNA metabolism (replication cycle, amplification) and the genomeediting tool CRISPR-Cas9 are purposefully discussed in order to address strategic possibility to design DNA-sensors based on immobilized DNA-enzymes. However, the immobilization of biologically active proteins on a gold carrier technique with the ability to detect viral or bacterial nucleic acids is individual for each DNA-modifying enzyme group, due to a different number of active sites, C and N terminal locations and arrangement, therefore, individual protocols based on the 'masking' of active sites should be elaborated for each enzyme.