1.
Prostaglandins
; 40(3): 283-96, 1990 Sep.
Article
in English
| MEDLINE
| ID: mdl-2247618
ABSTRACT
An analytical method for the determination of the PGE derivative nocloprost in plasma was developed combining the features of both radioimmunoassay and GC/MS. The antibody usually employed in nocloprost radioimmunoassay was coupled to Sepharose 4B and used as a stationary phase for the extraction of the drug. After appropriate derivatisation, nocloprost was determined by GC/MS in the negative ion-chemical ionisation mode. As an internal standard deuterated nocloprost was synthesized and added to the plasma samples before extraction. The extraction recovery was 94% and the limit of detection was 5 pg/ml. Intra- and interassay precision at 100 pg/ml was calculated as 3.3 and 4.0%, respectively.