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1.
Cell Biol Toxicol ; 22(2): 91-9, 2006 Mar.
Article in English | MEDLINE | ID: mdl-16528448

ABSTRACT

The biological activity of methanolic and aqueous extracts from dehydrated hypocotyls of Lepidium meyenii (Brassicaceae, vernacular name "maca"), was studied on rat hepatocytes and human breast cancer MCF-7 cells. The extracts did not exhibit cytotoxicity in hepatocyte primary cultures up to 10 mg/ml as measured by the MTT viability test, and lactate dehydrogenase (LDH) and aspartate aminotransferase (AST) leakage. Moreover, after 72 h, extracts inhibited LDH and AST leakage from the hepatocytes. When hepatocytes were intoxicated by t-butyl hydroperoxide, neither extract prevented oxidative damage. Both extracts showed weak antioxidant activity in the DPPH radical scavenging test with IC(50) values of 3.46 +/- 0.16 and 0.71 +/- 0.10 mg/ml, for aqueous and methanolic extracts, respectively. Thus, the observed effect on spontaneous enzyme leakage is probably mediated through mechanisms other than antioxidant activity. Both methanolic and aqueous extracts have shown estrogenic activity comparable with that of silymarin in MCF-7 cell line. Maca estrogenicity was exhibited in the range from 100 to 200 mug of extract per ml. The findings in the present study show that maca does not display in vitro hepatotoxicity. In contrast, a slight cytoprotective effect, probably not mediated by antioxidant capacity, was noted. Maca extracts exhibited estrogenic activity comparably to the effect of silymarin in MCF-7 cells.


Subject(s)
Breast Neoplasms/drug therapy , Drug Evaluation, Preclinical , Hepatocytes/drug effects , Lepidium/chemistry , Lepidium/toxicity , Plant Extracts/toxicity , Animals , Biphenyl Compounds/analysis , Cell Line, Tumor , Cell Proliferation , Cells, Cultured , Estrogens/pharmacology , Fatty Acids/analysis , Humans , Hydrazines/analysis , Male , Picrates , Plant Extracts/chemistry , Rats , Rats, Wistar , Steroids/analysis
2.
Teratology ; 59(3): 139-47, 1999 Mar.
Article in English | MEDLINE | ID: mdl-10194804

ABSTRACT

To study the mechanism of hyperthermia on the development of the rostral neural tube, we used a model in which closely-staged presomite 9.5-day rat embryos were exposed in culture to 43 degrees C for 13 min, and then cultured further for 12-48 hr. This treatment had little effect on the development of the rest of the embryo, but resulted in a spectrum of brain defects, the most severe being a lack of all forebrain and midbrain structures. Whole-mount in situ hybridisation was used to monitor the expression domains of Otx2, Emx2, Krox20, and hoxb1. These showed that there were no ectopic expression patterns, for any gene at any stage examined. Even in those embryos which apparently lacked all forebrain and midbrain structures, there were expression domains of Otx2 and Emx2 in the most rostral neural tissue, and these retained their nested dorso-ventral boundaries, showing that cells fated to form rostral brain were not wholly eliminated. Thus, heat-induced rostral neural tube truncation is of a quite different mechanism from the respecification proposed for retinoic acid, despite their very similar phenotypes. In the hindbrain region of treated embryos, we observed decreased intensity of Krox20, staining and an abnormal relationship developed between the position of hoxb1 expression and the otocyst and pharyngeal arches. In the most extreme cases, this domain was shifted to be more caudal than the rostral edge of the otocyst, while the otocyst retained its normal position relative to the pharyngeal arches. We interpret this as a growth imbalance between neuroepithelium and overlying tissues, perhaps due to a disruption of signals from the midbrain/hindbrain boundary.


Subject(s)
Brain/abnormalities , Central Nervous System/embryology , Gene Expression Regulation, Developmental , Genes, Homeobox , Hot Temperature , Rhombencephalon/embryology , Animals , Brain/embryology , Central Nervous System/abnormalities , Central Nervous System/metabolism , Culture Techniques , Embryonic and Fetal Development , In Situ Hybridization , Neural Crest/embryology , Neural Crest/metabolism , Rats , Rats, Wistar , Time Factors , Transcription Factors/genetics
3.
Int J Dev Biol ; 42(5): 737-40, 1998 Jul.
Article in English | MEDLINE | ID: mdl-9712529

ABSTRACT

Although hyperthermia is an established teratogen in all species studied and the cellular heat shock response is well known, the mechanisms of developmental deviation remain obscure. We have used a chick model system in which fertilized eggs containing embryos at presomite and/or early somite stages (HH 4-10) were exposed to 45 degrees C for 180 min. Six hours following treatment we did not observe any overt morphological disturbance, but at twelve hours following exposure (when controls reached HH 11-13) embryos exposed at late streak stages (HH 4-6) exhibited severe malformation of the head. Embryos exposed later (HH 6-9) manifested spina bifida at the thoracic and lumbosacral levels. Mirror image heart looping was also observed in 20% of these embryos. Paraxial mesoderm was apparently unaffected. Changes in cell proliferation and induced cell death preceded morphological changes. We used acridine orange and confocal laser microscopy to demonstrate that hyperthermia induced cell death in neural folds starting 6 h following treatment. To assess cell proliferation, we used BrdU incorporation for 4 h. Immunodetection on paraffin sections demonstrated that proliferation was inhibited 6 h after treatment. Heat-exposed embryos exhibited the heat shock response, with protein expression reaching a maximum 4-6 h following heat treatment. Malformed embryos showed an intense heat shock response for a further 6 h. The levels of induced heat shock proteins were similar in the affected neural tube and in the heart, where neither induced cell death nor malformations were observed.


Subject(s)
Chick Embryo/abnormalities , Heat-Shock Proteins/biosynthesis , Hyperthermia, Induced/adverse effects , Animals , Cell Death , Chick Embryo/cytology , Embryo, Nonmammalian/abnormalities , Embryo, Nonmammalian/cytology
4.
Folia Biol (Praha) ; 42(5): 231-4, 1996.
Article in English | MEDLINE | ID: mdl-8997640

ABSTRACT

A hyperthermic shock (43 degrees C/30 min) enhances in somite stages of the chick embryo development the activity of the caudal morphogenetic system, manifested by intensive growth of the embryonic trunk. The exposure also induced the synthesis of heat shock proteins (HSP 70). A single administration of a bioflavonoid quercetin dissolved in DMSO to chick embryos in stages HH 10-11 (10-14 somites) prior to heat exposure inhibited both the growth acceleration and the HSP induction.


Subject(s)
HSP70 Heat-Shock Proteins/metabolism , Quercetin/pharmacology , Animals , Chick Embryo , Heat-Shock Response
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