Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 20 de 22
Filter
Add more filters










Publication year range
1.
J Med Entomol ; 47(6): 1019-27, 2010 Nov.
Article in English | MEDLINE | ID: mdl-21175049

ABSTRACT

Acquisition of ticks by bird hosts is a central process in the transmission cycles of many tick-borne zoonoses, but tick recruitment by birds has received little direct study. We documented acquisition of Ixodes scapularis Say on birds at Fire Island, NY, by removing ticks from mist-netted birds, and recording the number of ticks on birds recaptured within 4 d of release. Eight bird species acquired at least 0.8 ticks bird(-1) day(-1) during the seasonal peak for at least one age class of I. scapularis. Gray Catbirds, Eastern Towhees, Common Yellowthroats, and Northern Waterthrushes collectively accounted for 83% of all tick acquisitions; and six individuals apportioned among Black-billed Cuckoo, Gray Catbird, Eastern Towhee, and Common Yellowthroat were simultaneously infested with both larvae and nymphs. Bird species with the highest acquisition rates were generally ground foragers, whereas birds that did not acquire ticks in our samples generally foraged above the ground. Tick acquisition by birds did not differ between deciduous and coniferous forests. Among the 15 bird species with the highest recruitment rates, acquisition of nymphs was not correlated with acquisition of larvae. Tick acquisition rates by individual bird species were not correlated with the reservoir competence of those species for Lyme borreliae. However, birds with high tick acquisition rates can contribute large numbers of infected ticks, and thus help maintain the enzootic cycle, even if their levels of reservoir competence are relatively low.


Subject(s)
Bird Diseases/parasitology , Ixodes/physiology , Tick Infestations/veterinary , Animals , Atlantic Ocean , Bird Diseases/epidemiology , Birds , Geography , Seasons , Tick Infestations/epidemiology , Tick Infestations/parasitology , Time Factors
2.
J Vector Ecol ; 35(1): 69-74, 2010 Jun.
Article in English | MEDLINE | ID: mdl-20618650

ABSTRACT

Mosquito host-seeking activity was studied using a custom-designed trap to explore: (1) at which time interval of the night adult mosquito abatement would be most effective, and (2) if there exists an avian-specific host-seeking preference. Overnight trials using traps baited with dry ice showed that Aedes taeniorhynchus (Wiedemann) was most active at dusk and was then captured throughout the night. In contrast, Culex spp. (Cx. pipiens (Linnaeus) and Cx. restuans (Theobald) delayed most activity until about two h after dusk and were then captured through the night. This pattern suggests that management activities directed at adult Culex spp. would be most effective if initiated well after sunset. Mosquito capture rates in traps baited with birds in net bags were significantly greater than those with empty net bags, indicating that mosquitoes were attracted to the birds and not incidentally being sucked in by the custom trap's strong fan motor (Wilcoxon matched-pairs signed-ranks test, n=24, t=30, p<0.05). Regression analysis showed that bird weight influenced mosquito attraction (r2=0.21, p=0.02). Trials with paired traps that contained different native bird species showed that Gray Catbirds, Dumatella carolinensis, attracted more mosquitoes than the heavier Northern Cardinals, Cardinalis cardinalis (paired samples t-test, t=2.58, df=7, p=0.04). However, attractiveness did not differ substantially among bird species, and Gray Catbirds did not attract more mosquitoes than all other birds combined as a group. American Robins, Turdus migratorius (n=4) were comparable in attractiveness to other bird species, but not enough American Robins were captured for a comprehensive study of mosquito avian preference.


Subject(s)
Birds/parasitology , Culicidae/virology , West Nile Fever/transmission , West Nile Fever/virology , Animals , Culicidae/growth & development , United States
3.
J Med Entomol ; 42(3): 445-9, 2005 May.
Article in English | MEDLINE | ID: mdl-15962798

ABSTRACT

Reservoir competence for the Lyme disease spirochete, Borrelia burgdorferi, was tested for six species of native North American birds: American robin, gray catbird, brown thrasher, eastern towhee, song sparrow, and northern cardinal. Wild birds collected by mist netting on Fire Island, NY, were held in a field laboratory in cages over water and locally collected larval ticks were placed on the birds, harvested from the water after engorgement, and tested for infection by direct fluorescentantibody staining after molting to the nymphal stage. American robins were competent reservoirs, infecting 16.1% of larvae applied to wild-caught birds, compared with 0% of control ticks placed on uninfected laboratory mice. Robins that were previously infected in the laboratory by nymphal feeding infected 81.8% of applied larvae. Wild-caught song sparrows infected 4.8% of applied larvae and 21.1% when infected by nymphal feeding. Results suggest moderate levels of reservoir competence for northern cardinals, lower levels for gray catbirds, and little evidence of reservoir competence for eastern towhees or brown thrashers. Lower infection rates in larvae applied to wild-caught birds compared with birds infected in the laboratory suggest that infected birds display temporal variability in infectiousness to larval ticks. Engorged larvae drop from birds abundantly during daylight, so the abundance of these bird species in the peridomestic environment suggests that they might contribute infected ticks to lawns and gardens.


Subject(s)
Birds/microbiology , Borrelia burgdorferi , Disease Reservoirs/veterinary , Animals , Larva/microbiology , Lyme Disease/transmission , Songbirds/microbiology , Ticks/growth & development , Ticks/microbiology
4.
Structure ; 10(1): 105-13, 2002 Jan.
Article in English | MEDLINE | ID: mdl-11796115

ABSTRACT

Methylaspartate ammonia lyase (MAL) catalyzes the magnesium-dependent reversible alpha,beta-elimination of ammonia from L-threo-(2S,3S)-3-methylaspartic acid to mesaconic acid. The 1.3 A MAD crystal structure of the dimeric Citrobacter amalonaticus MAL shows that each subunit comprises two domains, one of which adopts the classical TIM barrel fold, with the active site at the C-terminal end of the barrel. Despite very low sequence similarity, the structure of MAL is closely related to those of representative members of the enolase superfamily, indicating that the mechanism of MAL involves the initial abstraction of a proton alpha to the 3-carboxyl of (2S,3S)-3-methylasparic acid to yield an enolic intermediate. This analysis resolves the conflict that had linked MAL to the histidine and phenylalanine ammonia lyase family of enzymes.


Subject(s)
Ammonia-Lyases/chemistry , Citrobacter/enzymology , Protein Structure, Tertiary , Amino Acid Sequence , Ammonia-Lyases/metabolism , Binding Sites , Crystallography, X-Ray , Evolution, Molecular , Models, Molecular , Molecular Sequence Data , Molecular Structure , Protein Folding , Substrate Specificity
5.
Acta Crystallogr D Biol Crystallogr ; 57(Pt 12): 1922-4, 2001 Dec.
Article in English | MEDLINE | ID: mdl-11717518

ABSTRACT

Methylaspartate ammonia lyase (MAL) catalyses the reversible alpha,beta-elimination of ammonia from L-threo-(2S,3S)-3-methylaspartic acid to give mesaconic acid. Crystals of Citrobacter amalonaticus MAL have been obtained by the hanging-drop method of vapour diffusion using ammonium sulfate as the precipitant. Three crystal forms were obtained from identical crystallization conditions, two of which (forms A and B) diffract to high resolution, whilst the third form diffracted poorly. Crystals of form A diffract to beyond 2.1 A and have been characterized as belonging to one of the enantiomorphic space groups P4(1)22 or P4(3)22, with unit-cell parameters a = b = 66.0, c = 233.1 A, alpha = beta = gamma = 90 degrees and a monomer in the asymmetric unit. Crystals of form B diffract to beyond 1.5 A and belong to space group C222, with unit-cell parameters a = 128.3, b = 237.4, c = 65.8 A, alpha = beta = gamma = 90 degrees and a dimer in the asymmetric unit. Determination of the structure of MAL will be an important step in resolving current conflicts concerning the enzyme mechanism which differ between one which places MAL as a member of the superfamily of ammonia lyases whose catalytic activity requires a cofactor formed by post-translational modification of the enzyme and another which links MAL to the enolase superfamily.


Subject(s)
Ammonia-Lyases/chemistry , Citrobacter/enzymology , Crystallization , Crystallography, X-Ray , Protein Conformation
6.
Environ Manage ; 28(2): 207-24, 2001 Aug.
Article in English | MEDLINE | ID: mdl-11443385

ABSTRACT

During the 1980s, the exponential growth of laughing gull (Larus atricilla) colonies, from 15 to about 7600 nests in 1990, in the Jamaica Bay Wildlife Refuge and a correlated increase in the bird-strike rate at nearby John F. Kennedy International Airport (New York City) led to a controversy between wildlife and airport managers over the elimination of the colonies. In this paper, we review data to evaluate if: (1) the colonies have increased the level of risk to the flying public; (2) on-colony population control would reduce the presence of gulls, and subsequently bird strikes, at the airport; and (3) all on-airport management alternatives have been adequately implemented. Since 1979, most (2987, 87%) of the 3444 bird strikes (number of aircraft struck) were actually bird carcasses found near runways (cause of death unknown but assumed to be bird strikes by definition). Of the 457 pilot-reported strikes (mean = 23 +/- 6 aircraft/yr, N = 20 years), 78 (17%) involved laughing gulls. Since a gull-shooting program was initiated on airport property in 1991, over 50,000 adult laughing gulls have been killed and the number of reported bird strikes involving laughing gulls has declined from 6.9 +/- 2.9 (1983-1990) to 2.6 +/- 1.3 (1991-1998) aircraft/yr; nongull reported bird strikes, however, have more than doubled (6.4 +/- 2.6, 1983-1990; 14.9 +/- 5.1, 1991-1998). We found no evidence to indicate that on-colony management would yield a reduction of bird strikes at Kennedy Airport. Dietary and mark-recapture studies suggest that 60%-90% of the laughing gulls collected on-airport were either failed breeders and/or nonbreeding birds. We argue that the Jamaica Bay laughing gull colonies, the only ones in New York State, should not be managed at least until all on-airport management alternatives have been properly implemented and demonstrated to be ineffective at reducing bird strikes, including habitat alterations and increasing the capability of the bird control unit to eliminate bird flocks on-airport using nonlethal bird dispersal techniques. Because the gull-shooting program may be resulting in a nonsustainable regional population of laughing gulls (>30% decline), we also recommend that attempts be made to initiate an experimental colony elsewhere on Long Island to determine if colony relocation is a feasible management option.


Subject(s)
Accidents, Aviation/prevention & control , Aircraft , Birds , Conservation of Natural Resources , Animals , Diet , Humans , New York City , Population Dynamics , Reproduction
7.
Structure ; 8(8): 809-15, 2000 Aug 15.
Article in English | MEDLINE | ID: mdl-10997900

ABSTRACT

BACKGROUND: Membrane-bound ion pumps are involved in metabolic regulation, osmoregulation, cell signalling, nerve transmission and energy transduction. How the ion electrochemical gradient interacts with the scalar chemistry and how the catalytic machinery is gated to ensure high coupling efficiency are fundamental to the mechanism of action of such pumps. Transhydrogenase is a conformationally coupled proton pump linking a proton gradient to the redox reaction between NAD(H) and NADP(H). The enzyme has three components; dI binds NAD(H), dII spans the membrane and dIII binds NADP(H). RESULTS: The first crystal structure of a transhydrogenase dI component (from Rhodospirillum rubrum) has been determined at 2.0 A resolution. The monomer comprises two domains. Both are involved in dimer formation, and one has a Rossmann fold that binds NAD+ in a novel mode. The two domains can adopt different conformations. In the most closed conformation, the nicotinamide ring is expelled from the cleft between the two domains and is exposed on the outside of the protein. In this conformation it is possible to dock the structure of dI/NAD+ with that of a dIII/NADP+ complex to provide the first insights into the molecular basis of the hydride-transfer step. CONCLUSIONS: Analysis of the model of the dI/dIII complex identifies residues potentially involved in dI/dIII interaction and shows how domain motion in dI results in a shift in position of the nicotinamide ring of NAD+. We propose that this movement is responsible for switching between the forbidden and allowed states for hydride transfer during proton pumping.


Subject(s)
NADP Transhydrogenases/chemistry , Molecular Sequence Data , NADP Transhydrogenases/metabolism , Protein Binding , Protein Conformation , Protons , Rhodospirillum rubrum , Substrate Specificity
8.
Acta Crystallogr D Biol Crystallogr ; 56(Pt 9): 1170-2, 2000 Sep.
Article in English | MEDLINE | ID: mdl-10957636

ABSTRACT

Nicotinamide nucleotide transhydrogenase couples the exchange of a hydride-ion equivalent between NAD(H) and NADP(H) to the translocation of protons across an energy-transducing membrane. Peripheral components of 380 and 200 residues bind NAD(H) (dI) and NADP(H) (dIII), respectively, while a third component forms a membrane-spanning region (dII). The NAD(H)-binding component dI of Rhodospirillum rubrum transhydrogenase has been crystallized in a form which diffracts to beyond 3.0 A resolution and is in space group P2 or P2(1), with unit-cell parameters a = 69.3, b = 117.8, c = 106.6 A, beta = 107.2 degrees and two dimers in the asymmetric unit. The sequence of the dI component is similar to that of alanine dehydrogenase. A full structure determination will lead to important information on the mode of action of this proton pump and will permit the comparison of the structure-function relationships of dI with those of alanine dehydrogenase.


Subject(s)
Bacterial Proteins/chemistry , NADP Transhydrogenases/chemistry , Bacterial Proteins/isolation & purification , Bacterial Proteins/metabolism , Binding Sites , Crystallization , Crystallography, X-Ray , Escherichia coli/enzymology , Escherichia coli/genetics , NADP Transhydrogenases/isolation & purification , NADP Transhydrogenases/metabolism , Recombinant Proteins/chemistry , Recombinant Proteins/genetics , Recombinant Proteins/isolation & purification , Rhodospirillum rubrum/enzymology , Rhodospirillum rubrum/genetics
9.
Sleep ; 18(3): 180-7, 1995 Apr.
Article in English | MEDLINE | ID: mdl-7610314

ABSTRACT

Patients with obstructive sleep apnea (OSA) are often obese and, in common with obese patients generally, find it difficult to lose weight. Obstructive sleep apnea may be associated with changes in total daily energy expenditure that could contribute to obesity and complicate its management. To determine whether resting metabolic rate and the thermogenic effect of food are reduced in OSA, we have compared postabsorptive resting energy expenditure (REE) and dietary thermogenesis (DT) in 14 patients with moderate to severe symptomatic OSA and 14 control subjects matched for obesity. Anthropometrics, body composition analysis using bioelectrical impedance and indirect calorimetry using a metabolic cart and canopy system were performed in all subjects. Dietary thermogenesis after a liquid meal equivalent to 35% of REE was measured in 13 patients and 8 control subjects. Measurements were repeated after chronic (mean +/- SD 12 +/- 5 weeks) nasal continuous positive airway pressure (CPAP) therapy in 10 patients with OSA. Energy expenditure was expressed in terms of metabolic body size. The patients with OSA were heavier and had larger necks and a larger lean body mass (LBM) than controls, but the two groups were well matched for body mass index (BMI) and percent body fat. REE was greater in OSA patients than controls, but when corrected for LBM there was no difference between the two groups (27 +/- 3 vs. 28 +/- 4 kcal/kg). DT was similar in patients and controls (17 +/- 6 vs. 15 +/- 10%). REE/LBM was quite consistent among patients with OSA, regardless of body weight. REE and DT did not change following chronic nasal CPAP therapy. (ABSTRACT TRUNCATED AT 250 WORDS)


Subject(s)
Energy Metabolism , Obesity/metabolism , Sleep Apnea Syndromes/metabolism , Anthropometry , Body Mass Index , Calorimetry , Female , Humans , Male , Obesity/complications , Polysomnography , Positive-Pressure Respiration , Sleep Apnea Syndromes/complications , Sleep Apnea Syndromes/therapy
10.
Chest ; 103(4): 1038-44, 1993 Apr.
Article in English | MEDLINE | ID: mdl-8131435

ABSTRACT

We have measured caloric intake, energy expenditure, and the thermogenic effect of food in ten patients with stable COPD who had a history of involuntary weight loss over several years and were malnourished (< 85 percent ideal body weight). Each patient completed a 7-day food record. Indirect calorimetry was performed in the resting postabsorptive state. After placement of a nasoenteric tube, patients were randomly assigned to be refed or sham-fed (mean +/- SD, 16 +/- 3 days), following which, metabolic measurements were repeated. Indirect calorimetry was also performed before and after a large meal in each patient. Home caloric intake was 135 +/- 23 percent of resting energy expenditure. Resting energy expenditure was 94 +/- 16 percent of that predicted by the Harris-Benedict equation and did not change significantly during inpatient refeeding. Refeeding resulted in weight gain (2.4 +/- 1.9 kg, p < 0.02). A large meal caused substantial increases in energy expenditure (24 +/- 18 percent), carbon dioxide production (39 +/- 18 percent), and oxygen consumption (23 +/- 16 percent). We conclude that stable malnourished COPD patients consume adequate calories to meet average energy requirements and are not hypermetabolic. Inpatient refeeding by nocturnal nasoenteric infusion is well tolerated and results in weight gain, but the thermogenic effect of a large meal poses a considerable metabolic and ventilatory load that could precipitate acute respiratory failure.


Subject(s)
Energy Metabolism , Lung Diseases, Obstructive/complications , Nutrition Disorders/metabolism , Aged , Body Temperature Regulation , Calorimetry, Indirect , Energy Intake , Enteral Nutrition , Female , Humans , Lung Diseases, Obstructive/physiopathology , Male , Middle Aged , Nutrition Disorders/complications
12.
Am Rev Respir Dis ; 142(2): 283-8, 1990 Aug.
Article in English | MEDLINE | ID: mdl-2116747

ABSTRACT

We carried out a prospective randomized controlled trial to investigate the effects of short-term refeeding (16 days) in 10 malnourished inpatients with chronic obstructive pulmonary disease (COPD). Six patients were randomized to receive sufficient nasoenterically administered calories to provide a total caloric intake equal to 1,000 kcal above their usual intake. The other four patients were sham fed, receiving only 100 kcal more. Measurements of nutritional status, respiratory muscle strength and endurance, adductor pollicis function, and pulmonary function were performed initially and at study end. The refed group gained significantly more weight and showed significant increases in maximal expiratory pressure and mean sustained inspiratory pressure. There were no significant changes in the maximal inspiratory pressure or in adductor pollicis function. In malnourished inpatients with COPD, short-term refeeding leads to improvement in respiratory muscle endurance and in some parameters of respiratory muscle strength in the absence of demonstrable changes in peripheral muscle function.


Subject(s)
Enteral Nutrition , Lung Diseases, Obstructive/physiopathology , Muscle Contraction/physiology , Muscles/physiopathology , Nutrition Disorders/diet therapy , Respiratory Muscles/physiopathology , Aged , Energy Intake , Female , Humans , Lung Diseases, Obstructive/complications , Male , Middle Aged , Nutrition Disorders/complications , Prospective Studies , Randomized Controlled Trials as Topic
15.
Br J Urol ; 57(5): 574-8, 1985 Oct.
Article in English | MEDLINE | ID: mdl-4063738

ABSTRACT

The corpora cavernosa contain a fibrous skeleton composed of tunica albuginea with its fibrous columns, periarterial and perineural fibrous sheaths, and an intraparenchymal fibrous framework attached to all fibrous elements of the corpora cavernosa. This anatomical arrangement probably plays an important part in the firmness of the corpora cavernosa during erection. The fibrous framework, due to its numerous attachments to the deep surface of the tunica albuginea and its columns, when stretched during erection, prevents bending and deformation of the corpora cavernosa.


Subject(s)
Penile Erection , Penis/anatomy & histology , Adolescent , Adult , Aged , Elastic Tissue/anatomy & histology , Humans , Male , Middle Aged , Penis/blood supply
16.
J Urol ; 132(1): 44-6, 1984 Jul.
Article in English | MEDLINE | ID: mdl-6202895

ABSTRACT

The nerves of the corpora cavernosa have anatomical characteristics different from other nerves. The intracavernous nerves are located in fibrous tunnels into which numerous fibrous bundles establish attachments. These bundles are part of the fibrous meshwork included in the corpora cavernosa. We suspect that during erection, when the corpora fill with blood and high intracavernous pressure develops, the aforementioned meshwork of fibrous bundles attached to all fibrous elements of the corpora, including the tunica albuginea and the perineural fibrous tunnels, stretches and prevents compression of the intracavernous nerves.


Subject(s)
Penis/innervation , Adolescent , Adult , Humans , Male , Middle Aged , Muscle, Smooth/physiology , Nerve Fibers/physiology , Penis/physiology , Pressure , Staining and Labeling
18.
Biochem Genet ; 18(7-8): 727-42, 1980 Aug.
Article in English | MEDLINE | ID: mdl-7008778

ABSTRACT

Seaweed flies (Coelopa frigida) inhabit piles of decaying seaweed on the seashore. All populations so far studied have been found to be polymorphic at the alcohol dehydrogenase locus (Adh). This article reports an attempt to identify some of the forces of natural selection that may be maintaining this polymorphism First, the genetic determination of the rather complex isozyme system is described. Several inbred lines homozygous at the Adh locus were derived and the biochemical properties of their allozymes compared. Significant differences in both specific activities and thermal stabilities were found between ADH allozymes. A simple experiment is reported in which individuals with different Adh genotypes were cultured in competition with each other in the presence of elevated levels of ethanol. Although the presence of ethanol resulted in greater mortality, there is no evidence that it was selective with respect to the Adh genotypes. The possible relevance of these results to the maintenance of the Adh polymorphism is discussed.


Subject(s)
Alcohol Oxidoreductases/genetics , Diptera/genetics , Isoenzymes/genetics , Polymorphism, Genetic , Alcohol Oxidoreductases/metabolism , Animals , Ethanol/pharmacology , Genotype , Isoenzymes/metabolism
19.
Proc Natl Acad Sci U S A ; 74(5): 2031-5, 1977 May.
Article in English | MEDLINE | ID: mdl-266722

ABSTRACT

The proliferative fraction of mononucleated cells in differentiating chick embryonic wing muscle (day 11) was measured following continuous infusion of tritiated thymidine into the embryonic circulation. During progressively longer intervals of infusion of the isotopically labeled precursor, the percentage of cells that enter S becomes larger, reaching 92% at the longest time period measured (21 hr). These observations suggest that until they are withdrawn into nonreplicative muscle syncytia, virtually all of the single cells in differentiating embryonic muscle remain in the proliferative pool. Earlier calculations of the size of this pool in developing muscle, based on the percentage of cells in S during a brief pulse, indicated, however, that less than half of the mononucleated cells are still replicating. We therefore compared the size of the proliferative fraction determined by continuous labeling with the calculation of this same parameter using our own pulse-labeling data. We find that the calculation underestimates the size of the proliferative pool and is, in fact, an estimate of only that portion of the cells whose generation times cluster around the average. This underestimate is particularly pronounced in differentiating muscle in which, concomitant with myogenic fusion, the distribution of G1 times (and consequently generation times as well) becomes longer and more highly variable. Our results suggest that the mode of administering the labeled DNA precursor profoundly affects the measurement of cell cycle parameters in vivo when these parameters exhibit considerable variability. The data presented here do not support the notion that any sizeable fraction of the myoblast population is withdrawn from the cell cycle for any significant period of time prior to fusion.


Subject(s)
Cell Division , Muscles/cytology , Animals , Cell Fusion , Cell Nucleus/metabolism , Chick Embryo , Muscles/embryology , Thymidine/blood , Thymidine/metabolism , Wings, Animal/embryology
SELECTION OF CITATIONS
SEARCH DETAIL
...