ABSTRACT
The major histocompatibility complex (MHC) encodes several classes of protein vital to the regulation of the immune response. We have isolated 26 class I genes that map to this region in the C57BL/10 mouse and linked these into three gene clusters. The number of genes differs from the number found in the BALB/c strain and comparison of the organization of the class I genes in these two strains shows conserved regions and polymorphic regions which probably result from deletions, insertions and translocations within the MHC.
Subject(s)
Biological Evolution , Genes, MHC Class II , Major Histocompatibility Complex , Mice, Inbred C57BL/immunology , Animals , Base Sequence , Cloning, Molecular , DNA Restriction Enzymes , H-2 Antigens/genetics , Mice , Mice, Inbred Strains/immunology , Nucleic Acid Hybridization , Species SpecificitySubject(s)
Cloning, Molecular , Genes, MHC Class II , H-2 Antigens/genetics , Haploidy , Animals , Mice , Mice, Inbred AKR , Mice, Inbred BALB C , Polymorphism, GeneticSubject(s)
Genes , Globins/genetics , Histocompatibility Antigens/genetics , Major Histocompatibility Complex , Transcription, Genetic , Animals , Base Sequence , Cloning, Molecular , Cytotoxicity, Immunologic , DNA Restriction Enzymes , Humans , Leukemia, Experimental/metabolism , Methylation , Mice , Plasmids , Protein Biosynthesis , RNA, Messenger/genetics , T-Lymphocytes/immunologyABSTRACT
Polyhedra were solubilized under mild alkaline conditions and their structure during solubilization was studied by transmission and scanning electron microscopy. Some new structural features such as the extent of virion-packing in a polyhedral body and the cavities remaining in polyhedral bodies following the release of the occluded particles are demonstrated. Some other, previously reported features, such as the presence of polyhedral "membrane" and the crystalline array of the soluble polyhedral protein are accentuated at the various stages of solubilization.
Subject(s)
Inclusion Bodies, Viral/ultrastructure , Insect Viruses/ultrastructure , Lepidoptera/microbiology , Moths/microbiology , Animals , Crystallization , Insect Viruses/growth & development , Solubility , Viral Proteins , Virion/ultrastructureABSTRACT
The mol. wt. of the DNA from four nuclear polyhedrosis viruses isolated from Spodoptera littoralis, S. exempta, S. exigua and S. frugiperda were determined to be 84, 80, 68 and 74 X 10(6), respectively, by electron microscopy. The molecules were demonstrated to exist as double-stranded relaxed circular or supercoiled DNA, though linear forms of DNA were also observed.
