Electrochemical profiling of poliovirus particles inactivated by chemical method and ionizing radiation.

Electrochemical profiling of formaldehyde-inactivated poliovirus particles demonstrated a relationship between the D-antigen concentration and the intensity of the maximum amplitude currents of the poliovirus samples. The resultant signal was therefore identified as electrochemical oxidation of the surface proteins of the poliovirus. Using registration of electrooxidation of amino acid residues of the capsid proteins, a comparative electrochemical analysis of poliovirus particles inactivated by electrons accelerated with doses of 5 kGy, 10 kGy, 15 kGy, 25 kGy, 30 kGy at room temperature was carried out. An increase in the radiation dose was accompanied by an increase in electrooxidation signals. A significant increase in the signals of electrooxidation of poliovirus capsid proteins was detected upon irradiation at doses of 15-30 kGy. The data obtained suggest that the change in the profile and increase in the electrooxidation signals of poliovirus capsid proteins are associated with an increase in the degree of structural reorganization of surface proteins and insufficient preservation of the D-antigen under these conditions of poliovirus inactivation.

FORECASTING THE POPULATION MORTALITY RATE FROM CARDIOVASCULAR DISEASES AS A CONDITION OF THE ECONOMIC SECURITY OF THE STATE.

The article analyzes the population mortality rate in Ukraine from cardiovascular diseases (for example of Sumy region). The structure and dynamics of mortality among the region residents as a result of these diseases during 2012-2019 are also considered. Prognostic assessment the population mortality rate in Ukraine from diseases of the circulatory system (regional aspect). We used analytical, statistical research methods, the method of systematic approach and comparative analysis. Statistical processing was performed in accordance with generally accepted methods of variation statistics using the software STATISTICA 8.0. On the basis of the data from annual statistical reports of medical institutions of the Sumy region, subordinate to the Ministry of Public Health of Ukraine (2012-2019), statistical yearbooks, the forecast of mortality of the population from cardiovascular diseases was made. It is predicted that the mortality rate of the population from cardiovascular diseases will slightly increase by 2025 (by 0.5%). This moderate growth will be achieved due to a slight decrease in the population mortality rate from cerebrovascular diseases. But mortality from other cardiovascular pathologies will increase by 2025. Also, the work carried out the forecast of the population mortality rate from various cardiovascular diseases in the territorial respect. The forecast showed that mortality from cardiovascular diseases will increase in 10 of 19 administrative-territorial units of Sumy region. Likewise, the results of forecasting of the population mortality from cardiovascular diseases by Ukraine's region can be used by local authorities to prevent the growth level of mortality of the population from this pathology and to prevent of morbidity increase.

The effect of protease and Bacillus licheniformis on nutritional value of pea, faba bean, yellow lupin and narrow-leaved lupin in broiler chicken diets.

1. This study investigated the nutritional value (digestibility of ileal crude protein and amino acids (AAs)) and the AMEN value of legumes (pea, faba bean, yellow lupin, and narrow-leaved lupin) with or without protease. 2. Two hundred, one-day-old, male Ross 308 broiler chickens were randomly allocated to ten groups. Each group received one of five different protein raw materials - either peas, faba beans, yellow lupins or narrow-leaved lupins, with or without protease produced from Bacillus licheniformis. The birds were kept in individual cages. Nutrient digestibility was calculated using the difference method. The total duration of the study was 23 d, and the study involved two phases - accommodation phase (d 1-18) and experimental phase (d 18-23). 3. The effect of protease on response traits was not consistent between the legumes. The enzyme increased the digestibility of some AAs in the pea (arginine, leucine, phenylalanine, histidine, tyrosine, alanine, and proline), faba bean (lysine, arginine, glycine, and asparagine), and yellow lupin diets (lysine, valine, and serine), but this effect was not observed in the narrow-leaved lupin diet. The change in AMEN values due to protease addition was only statistically significant for the pea diet (P < 0.05), whereas in the case of other legume species, the difference was insignificant (P > 0.05). 4. Exogenous protease increased the digestibility of AAs present in pea, faba bean, and yellow lupin seeds but not in narrow-leaved lupin diets. The AMEN value of peas increased with protease supplementation, but no effect of protease was found for the AMEN values of faba bean and both the lupin diets.