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1.
Nat Prod Res ; : 1-8, 2024 Mar 22.
Article in English | MEDLINE | ID: mdl-38516731

ABSTRACT

The phytochemical composition of the Combretum trifoliatum leaves was studied for the first time. Two new triterpenoid saponins, named comtrifoside A (1) and comtrifoside B (2), together with two other saponins (3-4) were purified by variously chromatographic techniques. For the first time, compound 3 was informed from the Combretum genus, as well as all of the isolated compounds (1-4) were reported from C. trifoliatum. The chemical structures of them were clearly characterised using extensive UV-VIS, IR, HRMS-ESI, and NMR experimental data. The in vitro anti-inflammatory activities of 1 & 2 were examined against NO overproduction in LPS activation of RAW264.7.

2.
Eur J Med Chem ; 265: 116098, 2024 Feb 05.
Article in English | MEDLINE | ID: mdl-38171148

ABSTRACT

Overexpression of the chromosome 21 DYRK1A gene induces morphological defects and cognitive impairments in individuals with Down syndrome (DS) and in DS mice models. Aging neurons of specific brain regions of patients with Alzheimer's disease, DS and Pick's disease have increased DYRK1A immunoreactivity suggesting a possible association of DYRK1A with neurofibrillary tangle pathology. Epigallocatechin-3-gallate (EGCG) displays appreciable inhibition of DYRK1A activity and, contrary to all other published inhibitors, EGCG is a non-competitive inhibitor of DYRK1A. Prenatal exposure to green tea polyphenols containing EGCG protects from brain defects induced by overexpression of DYRK1A. In order to produce more robust and possibly more active analogues of the natural compound EGCG, here we synthetized new EGCG-like molecules with several structural modifications to the EGCG skeleton. We replaced the ester boun of EGCG with a more resistant amide bond. We also replaced the oxygen ring by a methylene group. And finally, we positioned a nitrogen atom within this ring. The selected compound was shown to maintain the non-competitive property of EGCG and to correct biochemical and behavioral defects present in a DS mouse model. In addition it showed high stability and specificity.


Subject(s)
Catechin/analogs & derivatives , Down Syndrome , Humans , Female , Pregnancy , Mice , Animals , Down Syndrome/drug therapy , Protein Serine-Threonine Kinases , Protein-Tyrosine Kinases , Mice, Transgenic , Cognition
3.
Biochem Biophys Res Commun ; 695: 149400, 2024 Feb 05.
Article in English | MEDLINE | ID: mdl-38160530

ABSTRACT

SETD2 (SET-domain containing protein 2) is a histone methyltransferase (HMT) of the SET family responsible for the trimethylation of K36 of histone H3, thus producing the epigenetic mark H3K36me3. Recent studies have shown that certain SET family HMTs, such as SMYD2, SMYD3 or SETDB1 can also methylate protein kinases and therefore be involved in signaling pathways. Here we provide structural and enzymatic evidence showing that SETD2 methylates the protein tyrosine kinase ACK1 in vitro. ACK1 is recognized as a major integrator of signaling from various receptor tyrosine kinases. Using ACK1 peptides and recombinant proteins, we show that SETD2 methylates the K514 residue of ACK1 generating K514 mono, di or tri-methylation. Interestingly, K514 is found in a "H3K36-like" motif of ACK1 which is known to be post-translationally modified and to be involved in protein-protein interaction. The crystal structure of SETD2 catalytic domain in complex with an ACK1 peptide further provides the structural basis for the methylation of ACK1 K514 by SETD2. Our work therefore strongly suggests that ACK1 could be a novel non-histone substrate of SETD2 and further supports that SET HMTs, such as SETD2, could be involved in both epigenetic regulations and cell signaling.


Subject(s)
Histones , Protein-Tyrosine Kinases , Protein-Tyrosine Kinases/metabolism , Histones/metabolism , Methylation , Histone-Lysine N-Methyltransferase/genetics , Protein Processing, Post-Translational
4.
Nat Prod Res ; 37(8): 1284-1291, 2023 Apr.
Article in English | MEDLINE | ID: mdl-34758682

ABSTRACT

One new hopane-type triterpene, indicuen (1), along with eight known compounds (2-9) were isolated from the n-hexane extract of the lichen Parmotrema indicum Hale. The chemical structures of isolated compounds were identified by interpretation of their spectroscopic data (1D, 2D NMR and HRESIMS) combined with DFT-NMR chemical shift calculations and subsequent assignment of DP4+ probabilities and by comparison with the literature. Indicuen represents for a rare hopane bearing a 1-carboxyethyl substituent at C-21 in lichens. Compounds 1-3 and 5-8 were evaluated for α-glucosidase inhibition and cytotoxicity against K562 and HepG2 cancer cell lines. Compounds 1, 5 and 7 exhibited moderate α-glucosidase inhibition with IC50 values of 201.1, 156.3 and 187.4 µM, respectively. Compound 1 also showed weak cytotoxicity toward K562 cell line while others showed no activity.


Subject(s)
Lichens , Parmeliaceae , Triterpenes , Molecular Structure , Vietnam , alpha-Glucosidases , Lichens/chemistry , Triterpenes/pharmacology , Pentacyclic Triterpenes
5.
Nat Prod Res ; 37(3): 455-461, 2023 Feb.
Article in English | MEDLINE | ID: mdl-34542362

ABSTRACT

From the Lasianthus bidoupensis stems, two new compounds, including one new 9,10-anthraquinone, lasibidoupin A (1), and one new 6,7-benzocoumarin, lasibidoupin B (2), together with one known compound, 11-O-methyldamnacanthol (3) were isolated using chromatographic method. Their structures were determined by extensive HRMS, and NMR assignments. Compound 3 was reported for the first time from this species. New compounds (1 & 2) were tested for the cytotoxicity against three human cancer cell lines (MCF-7, HeLa, and NCI-H460) by SRB assay. As results, 1 & 2 exhibited significant cytotoxic activity against all cancer cell lines (IC50 ranged from 0.058 ± 0.003 to 0.177 ± 0.014 µM).


Subject(s)
Antineoplastic Agents , Rubiaceae , Humans , Cell Line, Tumor , HeLa Cells , Antineoplastic Agents/chemistry , Rubiaceae/chemistry , Magnetic Resonance Spectroscopy
6.
Cell Metab ; 34(10): 1532-1547.e6, 2022 Oct 04.
Article in English | MEDLINE | ID: mdl-36198294

ABSTRACT

The hypothalamus is key in the control of energy balance. However, strategies targeting hypothalamic neurons have failed to provide viable options to treat most metabolic diseases. Conversely, the role of astrocytes in systemic metabolic control has remained largely unexplored. Here, we show that obesity promotes anatomically restricted remodeling of hypothalamic astrocyte activity. In the paraventricular nucleus (PVN) of the hypothalamus, chemogenetic manipulation of astrocytes results in bidirectional control of neighboring neuron activity, autonomic outflow, glucose metabolism, and energy balance. This process recruits a mechanism involving the astrocytic control of ambient glutamate levels, which becomes defective in obesity. Positive or negative chemogenetic manipulation of PVN astrocyte Ca2+ signals, respectively, worsens or improves metabolic status of diet-induced obese mice. Collectively, these findings highlight a yet unappreciated role for astrocytes in the direct control of systemic metabolism and suggest potential targets for anti-obesity strategy.


Subject(s)
Astrocytes , Hypothalamus , Animals , Astrocytes/metabolism , Energy Metabolism/physiology , Glucose/metabolism , Glutamic Acid/metabolism , Hypothalamus/metabolism , Mice , Obesity/metabolism , Paraventricular Hypothalamic Nucleus/metabolism
7.
Biomed Pharmacother ; 153: 113372, 2022 Sep.
Article in English | MEDLINE | ID: mdl-35809481

ABSTRACT

Protein tyrosine phosphatase 1B (PTP1B) is a critical regulator of different signalling cascades such as the EGFR pathway. The biological importance of PTP1B is further evidenced by knockout mice studies and the identification of recurrent mutations/deletions in PTP1B linked to metabolic and oncogenic alterations. Cisplatin is among the most widely used anticancer drug. The biological effects of cisplatin are thought to arise primarily from DNA damaging events involving cisplatin-DNA adducts. However, increasing evidence indicate that the biological properties of cisplatin could also rely on the perturbation of other processes such as cell signalling through direct interaction with certain cysteine residues in proteins. Here, we provide molecular, cellular and in vivo evidence suggesting that PTP1B is a target of cisplatin. Mechanistic studies indicate that cisplatin inhibited PTP1B in an irreversible manner and binds covalently to the catalytic cysteine residue of the enzyme. Accordingly, experiments conducted in cells and mice exposed to cisplatin showed inhibition of endogenous PTP1B and concomitant increase in tyrosine phosphorylation of EGFR. These findings are consistent with previous studies showing tyrosine phosphorylation-dependent activation of the EGFR pathway by cisplatin and with recent studies suggesting PTP1B inhibition by cisplatin and other platinum complexes. Importantly, our work provides novel mechanistic evidence that PTP1B is a protein target of cisplatin and is inhibited by this drug at molecular, cellular and in vivo levels. In addition, our work may contribute to the understanding of the pathways undergoing modulation upon cisplatin administration beyond of the established genotoxic effect of cisplatin.


Subject(s)
Cysteine , Protein Tyrosine Phosphatase, Non-Receptor Type 1 , Animals , Catalytic Domain , Cisplatin/pharmacology , Cysteine/metabolism , ErbB Receptors/metabolism , Mice , Mice, Knockout , Phosphorylation , Tyrosine/metabolism
8.
Int J Mol Sci ; 23(13)2022 Jun 24.
Article in English | MEDLINE | ID: mdl-35806064

ABSTRACT

Human protein tyrosine phosphatase 1B (PTP1B) is a ubiquitous non-receptor tyrosine phosphatase that serves as a major negative regulator of tyrosine phosphorylation cascades of metabolic and oncogenic importance such as the insulin, epidermal growth factor receptor (EGFR), and JAK/STAT pathways. Increasing evidence point to a key role of PTP1B-dependent signaling in cancer. Interestingly, genetic defects in PTP1B have been found in different human malignancies. Notably, recurrent somatic mutations and splice variants of PTP1B were identified in human B cell and Hodgkin lymphomas. In this work, we analyzed the molecular and functional levels of three PTP1B mutations identified in primary mediastinal B cell lymphoma (PMBCL) patients and located in the WPD-loop (V184D), P-loop (R221G), and Q-loop (G259V). Using biochemical, enzymatic, and molecular dynamics approaches, we show that these mutations lead to PTP1B mutants with extremely low intrinsic tyrosine phosphatase activity that display alterations in overall protein stability and in the flexibility of the active site loops of the enzyme. This is in agreement with the key role of the active site loop regions, which are preorganized to interact with the substrate and to enable catalysis. Our study provides molecular and enzymatic evidence for the loss of protein tyrosine phosphatase activity of PTP1B active-site loop mutants identified in human lymphoma.


Subject(s)
Lymphoma, B-Cell , Protein Tyrosine Phosphatase, Non-Receptor Type 1/genetics , Catalytic Domain , Humans , Lymphoma, B-Cell/genetics , Mutation , Phosphorylation , Protein Tyrosine Phosphatase, Non-Receptor Type 1/metabolism , Tyrosine/metabolism
9.
Antioxidants (Basel) ; 11(5)2022 05 23.
Article in English | MEDLINE | ID: mdl-35624894

ABSTRACT

The Aryl hydrocarbon Receptor (AhR) is a xenobiotic sensor in vertebrates, regulating the metabolism of its own ligands. However, no ligand has been identified to date for any AhR in invertebrates. In C. elegans, the AhR ortholog, AHR-1, displays physiological functions. Therefore, we compared the transcriptomic and metabolic profiles of worms expressing AHR-1 or not and investigated the putative panel of chemical AHR-1 modulators. The metabolomic profiling indicated a role for AHR-1 in amino acids, carbohydrates, and fatty acids metabolism. The transcriptional profiling in neurons expressing AHR-1, identified 95 down-regulated genes and 76 up-regulated genes associated with neuronal and metabolic functions in the nervous system. A gene reporter system allowed us to identify several AHR-1 modulators including bacterial, dietary, or environmental compounds. These results shed new light on the biological functions of AHR-1 in C. elegans and perspectives on the evolution of the AhR functions across species.

10.
Nat Prod Res ; 36(13): 3429-3434, 2022 Jul.
Article in English | MEDLINE | ID: mdl-33356561

ABSTRACT

Chemical investigation of the lichen Parmotrema tinctorum (Nyl.) Hale led to the isolation of two new phenolic compounds, 2-ethylhexyl orsellinate (1) and tinctorinone (2). The structures were determined by analysis of their MS and NMR data as well as by comparison with literature data. The 2-ethylhexyl ester group of 2-ethylhexyl orsellinate is uncommon among lichen metabolites. Tinctorinone revealed strong inhibition towards α-glucosidase.


Subject(s)
Lichens , Parmeliaceae , Asian People , Humans , Lichens/chemistry , Parmeliaceae/chemistry , Phenols/chemistry
11.
Nat Prod Res ; 36(8): 2037-2042, 2022 Apr.
Article in English | MEDLINE | ID: mdl-33213224

ABSTRACT

Further phytochemical investigation on P. tsavoense led to one new meta-depsidone, parmosidone K together with one known compound, barbatic acid. Their structures were determined by 1D and 2D NMR analysis, high resolution mass spectroscopy, and comparison their NMR data with those reported in literatures. Parmosidone K was evaluated for α-glucosidase inhibition and revealed the powerful activity with IC50 value of 3.12 µM.


Subject(s)
Lichens , Parmeliaceae , Depsides/chemistry , Lactones/chemistry , Lichens/chemistry
12.
Nat Prod Res ; 36(8): 1973-1979, 2022 Apr.
Article in English | MEDLINE | ID: mdl-33096957

ABSTRACT

From the leaves of Ricinus communis Linn., one new alkaloid, named ricicomin A (1) together with three known ones, ricinine (2), N-demethylricinine (3) and 4-[2-formyl-5-(methoxymethyl)-1H-pyrrol-1-yl]butanoic acid (4) were justified by repeated chromatographic methods. Their structures were determined by comprehensive IR, HR-ESI-MS and NMR analyses. Compound 4 was identified for the first time from the genus Ricinus. DFT-NMR chemical shift calculations and subsequent DP4+ probability methods were applied to confirm the chemical structure of 1. Compounds 1-3 did not display cytotoxic effect against three human cancer cell lines (MCF-7, HepG2 and HeLa) using SRB assay.


Subject(s)
Alkaloids , Ricinus , Alkaloids/chemistry , Humans , Molecular Structure , Plant Extracts/chemistry , Plant Leaves/chemistry , Ricinus/chemistry
13.
Nat Prod Res ; 36(8): 1934-1940, 2022 Apr.
Article in English | MEDLINE | ID: mdl-33107343

ABSTRACT

Three new diphenyl ethers, named praesorethers E, F and G (1, 2 and 3), were isolated from the lichen Parmotrema praesorediosum. Their chemical structures were elucidated on the basis of extensively spectroscopic analysis including HR-ESI-MS and NMR as well as comparison with previously published data. These compounds were evaluated for the cytotoxicity against three human cancer cell lines (HeLa, NCI-H460 and MCF-7) using SRB assay. As results, 1 and 2 exhibited weak cytotoxic activity against three tested cancer cell lines with the inhibitive percentage of 64-79.9% at the concentration of 100 µg/mL while 3 was inactive.


Subject(s)
Lichens , Parmeliaceae , Humans , Lichens/chemistry , Molecular Structure , Parmeliaceae/chemistry , Phenyl Ethers
14.
Nat Prod Res ; 36(8): 2009-2014, 2022 Apr.
Article in English | MEDLINE | ID: mdl-33155492

ABSTRACT

Three new phenolic compounds, peruvinides A-C were isolated from the lichen Ramalina peruviana Ach. (Ramalinaceae). Their structures were unambiguously determined by extensive spectroscopic analyses and comparison with literature data. Peruvinides A and B bearing unusual moieties were found for the first time among lichen metabolites.


Subject(s)
Ascomycota , Lichens , Ascomycota/chemistry , Lichens/chemistry , Phenols/metabolism
15.
Nat Prod Res ; 36(9): 2263-2269, 2022 May.
Article in English | MEDLINE | ID: mdl-33034223

ABSTRACT

Chemical investigation of the lichen Usnea ceratina Arch led to the isolation of five depsidones, including one new compound ceratinalone (1) along with four known compounds bailesidone (2), stictic acid (3), 8'-O-methylstictic acid (4) and 8'-O-ethylstictic acid (5). The structures were determined by analysis of their MS and NMR data as well as by comparison with literature values. Compounds 1 and 4 were evaluated the cytotoxic activity against HeLa (human epithelial carcinoma), NCI-H460 (human lung cancer), HepG2 (liver hepatocellular carcinoma), and MCF-7 (human breast cancer) cell lines, showing the moderate activity.


Subject(s)
Lichens , Parmeliaceae , Usnea , Animals , Ascomycota , Depsides/chemistry , Depsides/pharmacology , Humans , Lactones , Usnea/chemistry
16.
Nat Prod Res ; 36(14): 3705-3712, 2022 Jul.
Article in English | MEDLINE | ID: mdl-33576270

ABSTRACT

A novel C43-spiroterpenoid, reticulatin (1), was isolated from the lichen Parmotrema reticulatum (Taylor) M. Choisy (Parmeliaceae). Five previously-reported compounds were also isolated: zeorin (2), leucotylin (3), lupeol (4), betulinic acid (5), and dihydroreynosin (6). The structures were elucidated by 1D, 2D NMR, and HRESIMS spectroscopy and comparison with the literature. We propose that reticulatin is a biosynthetic product of fusicoccadiene and vinapraesorediosic acid A via Diels-Alder addition. Reticulatin is the first C43-spiroterpenoid identified from lichen metabolites. All compounds were evaluated for inhibition of α-glucosidase. Compound 1 showed the most potent inhibition, with an IC50 value of 3.90 µM, much lower than that of the acarbose positive control (IC50 165 µM).


Subject(s)
Lichens , Parmeliaceae , Lichens/chemistry , Parmeliaceae/chemistry , Vietnam , alpha-Glucosidases
17.
Nat Prod Res ; 36(15): 3945-3950, 2022 Aug.
Article in English | MEDLINE | ID: mdl-33749458

ABSTRACT

Two new compounds, comprising one dibenzofuran, named usneaceratin A (1), and one phenolic acid, named usneaceratin B (2), together with one known dibenzofuran, isousnic acid (3), and two known phenolics, orsellinic acid (4) and methyl orsellinate (5) were clarified from the lichen Usnea ceratina using variously chromatographic methods. Their structures were testified by comprehensive HR-ESI-MS, and NMR spectroscopic analysis, and comparison with published data. Their α-glucosidase inhibitory activity of all compounds was measured. Usneaceratin B (2) possessed better inhibition against α-glucosidase enzyme (IC50 value of 41.8 µM) than the standard drug acarbose (IC50 value of 214.50 µM).


Subject(s)
Lichens , Parmeliaceae , Usnea , Dibenzofurans , Glycoside Hydrolase Inhibitors/chemistry , Glycoside Hydrolase Inhibitors/pharmacology , Lichens/chemistry , Parmeliaceae/chemistry , Usnea/chemistry , alpha-Glucosidases
18.
Nat Prod Res ; 36(15): 3865-3871, 2022 Aug.
Article in English | MEDLINE | ID: mdl-33656403

ABSTRACT

Two new phenolic compounds, cristiferides A-B (1-2) together with six known compounds, 2,4-dihydroxyphthalide (3), lecanoric acid (4), orsellinic acid (5), 5-chloroorsellinic acid (6), methyl haematommate (7), and methyl ß-orsellinate (8) were isolated from the lichen Parmotrema cristiferum (Taylor) Hale (Parmeliaceae). The structures of isolated compounds were identified from its spectroscopic data and by comparison with the literatures. Compounds 1-3 and 6-8 were evaluated for alpha-glucosidase inhibition. Compounds 2 and 7 revealed potent activity with IC50 values of 72.66 µM and 48.73 µM, respectively.


Subject(s)
Lichens , Parmeliaceae , Lichens/chemistry , Phenols/chemistry , Phenols/pharmacology , Vietnam
19.
J Asian Nat Prod Res ; 24(2): 190-195, 2022 Feb.
Article in English | MEDLINE | ID: mdl-33794680

ABSTRACT

From the lichen Parmotrema praesorediosum, one new diphenyl peroxide, named praesordin A (1), together with four depsidones, including virensic acid (2), protocetraric acid (3), 8'-O-methylprotocetraric acid (4), and furfuric acid (5) were purified. Their structures were chacracterized using extensive HR-ESI-MS and NMR spectroscopic methods. The isolated compounds (2-5) possessed stronger α-glucosidase inhibitory activity (IC50 = 43.7-110.1 µM) than the standard drug acarbose (IC50 = 214.5 µM).


Subject(s)
Glycoside Hydrolase Inhibitors , Lichens , Peroxides , Biphenyl Compounds/pharmacology , Glycoside Hydrolase Inhibitors/pharmacology , Lichens/chemistry , Molecular Structure , Parmeliaceae/chemistry , Peroxides/pharmacology , alpha-Glucosidases
20.
Nat Prod Res ; 36(19): 4879-4885, 2022 Oct.
Article in English | MEDLINE | ID: mdl-33823690

ABSTRACT

Chemical investigation of the lichen Parmotrema indicum Hale led to the isolation of one new diphenyl ether, parmetherine D (1), along with eight known compounds (2-9). The structures were determined by analysis of MS and NMR data and by comparison with the literature. Compounds 1, 2, and 7 were evaluated for α-glucosidase inhibition. Only compound 1 exhibited significant inhibition.[Formula: see text].


Subject(s)
Lichens , Parmeliaceae , Lichens/chemistry , Parmeliaceae/chemistry , Phenyl Ethers , Vietnam , alpha-Glucosidases
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