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1.
Drug Metab Dispos ; 32(6): 620-5, 2004 Jun.
Article in English | MEDLINE | ID: mdl-15155553

ABSTRACT

Organic anion transporters (Oats) mediate the initial step of active renal excretion, specifically substrate uptake into proximal tubule cells. Despite extensive characterization of rat Oats, mouse Oat expression patterns are virtually unknown. This study was designed to identify basal expression patterns of mouse Oat1 (Slc22a6), Oat2 (Slc22a7), and Oat3 (Slc22a8) mRNA, compare these patterns with those in rat, and characterize postnatal development of mouse Oat mRNA. Tissues were collected from adult male and female 129J and C57BL/6 mice, and male and female C57BL/6 mice 0 to 40 days of age. Oat mRNA levels were determined by branched DNA signal amplification. Mouse Oat1 mRNA was primarily expressed in kidney of both strains, with male predominance. Mouse Oat2 mRNA levels were highest in kidney of both strains without gender predominance. In both strains, Oat3 mRNA was highest in kidney, and liver expression was male-predominant. However, only 129J mice had higher Oat3 mRNA levels in female kidney than in male kidney. During postnatal development, both Oat1 and Oat2 mRNA levels began to rise after 25 days of age. Oat3 mRNA levels rose gradually from birth through 40 days of age. Oat2 mRNA increased 30-fold during the first 40 days, whereas Oat1 and Oat3 increased about 2-fold. The most notable species differences in Oat mRNA expression were a lack of Oat2 female predominance in mouse kidney and a less dramatic Oat3 male predominance in mouse liver. With the exception of a significant species difference in Oat2 expression, many similarities were found between rat and mouse Oat mRNA levels.


Subject(s)
Organic Anion Transport Protein 1/genetics , Organic Anion Transporters/genetics , Animals , Branched DNA Signal Amplification Assay , Female , Gene Expression Regulation, Developmental , Kidney/growth & development , Kidney/metabolism , Male , Mice , Mice, Inbred C57BL , Organic Anion Transport Protein 1/biosynthesis , Organic Anion Transporters/biosynthesis , RNA, Messenger/biosynthesis , Rats , Rats, Sprague-Dawley , Sequence Analysis, DNA , Sex Factors
2.
Drug Metab Dispos ; 31(5): 559-64, 2003 May.
Article in English | MEDLINE | ID: mdl-12695343

ABSTRACT

Messenger RNA levels of rat organic anion transporter 1 (Oat1; Slc22a6) and Oat2 (Slc22a7) in kidney and Oat3 (Slc22a8) in liver are gender-predominant. Oat1 and Oat3 are male-predominant, whereas Oat2 is female-predominant. Gonadectomized and hypophysectomized (HX) rats were studied to determine whether sex steroids and/or growth hormone (GH) are responsible for these gender-divergent patterns. GH was administered to HX rats by two daily injections (simulating male secretion) or continuous infusion (simulating female secretion). Oat1 mRNA levels, normally higher in male than female kidney, were lowered by gonadectomy and HX in male rats, but not in females. Additionally, GH injections or infusion did not alter Oat1 levels in HX rats. Oat2 mRNA levels, typically much higher in female than in male kidney, were unaffected by gonadectomy. However, HX dramatically decreased Oat2 in female kidney without altering male levels. GH administered by continuous infusion increased Oat2 in kidneys of both HX male and female rats, whereas injections had no affect. Gonadectomy reduced Oat3 mRNA levels in male livers without affecting levels in female livers. In contrast, HX decreased male and elevated female Oat3 mRNA. GH injections did not significantly change Oat3 mRNA levels in HX rats, but infusion decreased Oat3 mRNA in liver. In conclusion, androgens, but not GH, are responsible for the Oat1 mRNA gender difference in kidney; the female GH secretion pattern is responsible for the Oat2 mRNA gender difference in kidney; and both androgens and the female GH secretion pattern are responsible for the Oat3 mRNA gender difference in liver.


Subject(s)
Endocrine System/physiology , Organic Anion Transport Protein 1/biosynthesis , Organic Anion Transporters, Sodium-Independent/biosynthesis , Animals , Aryl Hydrocarbon Hydroxylases/metabolism , Cytochrome P450 Family 2 , Female , Growth Hormone/pharmacology , Kidney/growth & development , Kidney/metabolism , Liver/growth & development , Liver/metabolism , Male , Orchiectomy , Ovariectomy , RNA, Messenger/biosynthesis , Rats , Rats, Sprague-Dawley , Sex Factors , Steroid 16-alpha-Hydroxylase/metabolism , Steroid Hydroxylases/metabolism
3.
J Pharmacol Exp Ther ; 301(1): 145-51, 2002 Apr.
Article in English | MEDLINE | ID: mdl-11907168

ABSTRACT

Rat organic anion transporter 1 (Oat1), Oat2, and Oat3, members of the organic anion transporter family, transport some organic anions across cellular membranes. Previously, highest Oat1 and Oat3 mRNA expression was reported in kidney and Oat2 in liver. However, gender and developmental differences in Oat expression remain unknown. This study describes gender- and age-specific patterns of rat organic anion transporter expression in various tissues. Oat mRNA expression was evaluated in adult male and female Sprague-Dawley rat tissues, and developmental expression was also determined in kidneys of Sprague-Dawley rats ranging in age from days 0 through 45. Expression was quantified using branched-DNA signal amplification. Oat1 mRNA expression was primarily observed in kidney. Surprisingly, Oat2 mRNA expression was also highest in kidney rather than in liver. Moreover, considerably higher Oat2 levels were seen in female kidney as compared with male. Finally, Oat3 mRNA expression was highest in kidney of both genders, whereas a male-predominant pattern was observed in liver. At birth, all kidney Oat mRNA levels were low. Renal Oat1 expression gradually increased throughout development, approaching adult levels at 30 days of age, where at days 40 and 45 Oat1 levels were greater in males than females. Oat2 expression in kidney was minimal through day 30 but increased dramatically at day 35 in females only. Lastly, Oat3 mRNA expression in kidney matured earliest, rapidly increasing from birth through day 10. These data indicate that Oat mRNA expression is primarily localized to the kidney, and observed expression patterns may explain some previously recognized age- and gender-dependent toxicities associated with chemical exposure.


Subject(s)
Aging/metabolism , Organic Anion Transport Protein 1/biosynthesis , Organic Anion Transporters, Sodium-Independent/biosynthesis , Animals , Blotting, Northern , DNA/analysis , DNA/biosynthesis , Female , Kidney/drug effects , Kidney/growth & development , Kidney/metabolism , Liver/growth & development , Liver/metabolism , Male , Oligonucleotide Probes , Pregnancy , RNA, Messenger/biosynthesis , Rats , Rats, Sprague-Dawley , Sex Characteristics , Tissue Distribution
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