[Biophysical parameters of erythrocyte membranes and mechanisms of interaction with non-opioid analgesics under acute pain syndrome].

Methods of fluorescent probing, spectrophotometry and microcalorimetry were applied to investigate the alterations in biophysical parameters of erythrocytes membranes, and specifically microviscosity, surface charge, molecular organization of lipid bilayer and lipid-protein interactions under conditions of acute pain syndrome produced by experimental chemical lesion. The distinctive features of non-opiod analgesics interactions and binding to the erythrocytes membranes of rats subjected to acute nociceptive pain accompanied with oxidative stress development were investigated. The abilities of analgesics under research, and namely paracetamol, aspirin, phenazone, ketorolac, pyrodazole, ketoprofenum, natrium mefenaminate, indometacin, nimesulide to make up physico-chemical complexes with lipoperoxidation modified erythrocytes surface and protein-lipid bilayer showed marked changes. The significance of oxidative damage of biophase under conditions of acute pain syndrome for analgesics effective pharmacodynamics and pharmacokinetics realization is under consideration.

[Effect of isonicotinic acid derivates on reproduction of Epstein-Barr virus].

Current approaches to the treatment of herpes infection, particularly Epstein-Barr virus (EBV), include the use of etiotropic medicines, as well as sensitizing therapy. This virus plays an important role in the etiology of nasopharyngeal carcinoma, adenocarcinoma of the parotid glands, gastric carcinoma, Burkitt's lymphoma and lymphoproliferative syndromes [1, 2, 3]. The spectrum of drugs active against EBV remains very limited, and gancyclovir and acyclovir are used in medical practice, so the search of new compounds active against EBV remains urgent. The purpose of this work was to study antiEBV activity of isonicotinic acid derivatives in the cultures of lymphoblastoid Raji cells, B95-8, Namalwa. The indices of cytotoxicity (CC50) which amounted to 840, 1250 and 3000 microg/ml and the concentration of drugs, which inhibit the virus (IC50) reproduction is 0.1, 2.5 and 50 microg/ml, respectively, in cell cultures were identified. It was detected, the drug 4-(n-benzyl)aminocarbonyl-1-methylpyridinium iodide (PV-1) had an ability to inhibit reproduction of the Epstein-Barr virus in all studied cells cultures. The compounds PV-2 and PV-10 were less toxic in respect of the initial preparation PV-1, but their antiviral activity was manifested at 25 and 500 times higher concentrations. It, respectively, influenced the decrease of their selectivity index, which was 8400 for PV-1, 400 and 440--for PV-2 and PV-10. These studies suggest possible ways of further modification of the PV-1 molecule to create highly specific inhibitors of Epstein-Barr virus. The paper is presented in Ukrainian.

[Cytostatic effects of bifolar and chlofiden. Effect on protein synthesis and cell cycle]. / Tsitostaticheskie éffekty bifolara i khlofidéna. Vliianie na sintez belka i kletochnyi tsikl.

Study of influence of new antitumor preparations bifolar and chlofiden on rat liver protein biosynthesis in vivo and on sarcoma-45 in vitro was carried out using labeled precursor 3H-leucine. It was shown that the preparations inhibited protein synthesis in normal and malignant tissues. Mitotic cycle and its phases were investigated with 3H-tymedin. It was established that bifolar influenced all the phases of mitotic cycle and the sensitive phases of the mitosis itself, which are connected with the redistribution of genetic material.

[Experience gained with the drug amizon in the treatment of patients with mumps, with special reference to its effects on some biochemical markers]. / Opyt primeneniia amizona v lechenii bol'nykh épidermicheskim parotitom i ego vliianie na nekotorye biokhimicheskie pokazateli.

Effectiveness was studied of a new drug amyzonum in a complex therapy of mumps in 149 patients versus the group of 177 patients having been treated with conventional methods only. A positive effect was shown of the drug on clinical indices, with the incidence of complications having gotten lower, biochemical indices having returned to normal.

[The efficacy of amizon in the combined treatment of patients with epidemic parotitis]. / Effektivnost' amizona v kompleksnom lechenii bol'nykh épidemicheskim parotitom.

Efficacy was studied of a new anti-inflammatory interferon-inducing drug preparation amizon in a combined therapy of epidemic parotiditis in 118 patients versus the group of comparison consisting of 147 patients. A positive effect was ascertained of the drug on clinical parameters, decrease in the incidence rate and in severity of complications (orchitis, pancreatitis), immune indices.

[Amizon in the treatment of nervous system involvement in herpes zoster]. / Amizon v lechenii porazhenii nervnoi sistemy pri opoiasyvaiushchem gerpese.

The authors submit results of treatment of patients having suffered from postherpetic pain syndrome (herpetic ganglionitis of the cervical, thoracic, lumbosacral localization, postherpetic intercostal neuralgia, ganglionitis of the trigeminal nerve). In viral diseases, amizone has marked analgetic, antiinflammatory, and immunomodulating effects. The drug is well tolerated by patients, is not associated with side effects under prescribed doses (0.25-0.75 as one dose on a three- or four-times daily schedule during the course of treatment lasting three or four weeks). The drug preparation in question is less effective in the treatment of chronic recurring post-therapeutic intercostal neuralgias, radiculalgia.

[The prospects and problems of drug-induced anesthesia]. / Perspektivy i problemy lekarstvennogo obezbolivaniia.

The problem of pain has a major medico-social significance and necessitates detailed investigation both from the position of etiology and pathogenesis and new therapy methods. Among many methods of pain control drug-induced anesthesia is of great importance. The multiplicity of pain requires optimal use of analgetics and their combination with other agents strictly in coordination with individual needs of the patient. Special anesthesia systems are to be designed for this purpose.

[Analysis of the role of calcium in analgesic action of non-narcotic analgesics and calcium channel blockers]. / Analiz roli kal'tsiia v obezbolivaiushchem deistvii nenarkoticheskikh anal'getikov i blokatorov kal'tsievykh kanalov.

In the mice hot-plate test we have compared analgesic effect of calcium channel blockers and new non-narcotic analgesic antiinflammatory agent PV-107: verapamil > fenigidin > PV-107. Simultaneously we have shown strong correlation (r - 0.82) between analgesic effect and 45Ca2+ efflux of cardiac membrane in depolarizing media in vitro.