ABSTRACT
This work presents the results of studying the molecular characteristics of parasitic tapeworms Echinococcus canadensis. The helminths were discovered during the autopsy of a wolf (Canis lupus Linnaeus, 1758) killed by hunters in the Kirov oblast in 2021. A molecular phylogenetic study was performed by analyzing the sequence of a fragment of the first subunit of the mitochondrial cytochrome oxidase gene (CoxI). It was found that the detected echinococci belong to the G10 genotype of E. canadensis, which is common in wolves in the northern territories of the Holarctic. We discovered four positions at which the substitutions characteristic only of this genotype are revealed. A substitution at one of the positions that is characteristic exclusively for the representatives of the G10 genotype found in Russia and Finland was also discovered.
ABSTRACT
Statistical methods confirmed that the dietary intake of traditionally made meat from marine mammals and polar bear could cause Trichinella infection in the residents of the communities of the Chukotka Peninsula.
Subject(s)
Antigens, Helminth/analysis , Meat Products/parasitology , Trichinella/physiology , Trichinellosis/ethnology , Trichinellosis/transmission , Animals , Humans , Meat Products/analysis , Population Groups , Seals, Earless/parasitology , Siberia/epidemiology , Statistics, Nonparametric , Trichinellosis/diagnosis , Trichinellosis/parasitology , Ursidae/parasitology , Walruses/parasitologyABSTRACT
Gastropods were established to be able to absorb decapsulated and encapsulated Trichinella larvae located in muscle tissue. Trichinella larvae remain viable and infectious for 24 to 48 hours after passing through the gastrointenstinal tract of gastropods.
Subject(s)
Snails/parasitology , Trichinella/physiology , Trichinellosis/transmission , Trichinellosis/veterinary , Animals , Deer/parasitology , Disease Vectors , Gastrointestinal Tract/parasitology , Humans , Larva/physiology , Mice , Trichinellosis/parasitologySubject(s)
Antigens/isolation & purification , Trichinella/isolation & purification , Trichinellosis/epidemiology , Trichinellosis/parasitology , Adult , Animals , Antigens/blood , Arctic Regions , Female , Humans , Male , Mammals/parasitology , Meat/parasitology , Middle Aged , Russia , Trichinella/pathogenicity , Trichinellosis/bloodABSTRACT
Epizootological surveys on the Arctic coasts of the Russian Federation revealed 8 terrestrial andmarine mammal species that were Trichinella carriers. The infection rate varied with the animal species from 1.6 to 92.8%. Analysis of the taxonomic affiliation of Trichinella isolated from the muscles of the terrestrial and marine mammals indicated that the Trichinella species T. nativa was widespread in the arctic areas of the Russian Federation. Analysis of sequences in the Cob gene of mtDNA revealed nucleotide differences between several isolates of this species.
Subject(s)
DNA, Mitochondrial/genetics , Electron Transport Complex IV/genetics , Trichinella/genetics , Trichinellosis/epidemiology , Trichinellosis/veterinary , Animals , Arctic Regions , Base Sequence , Cetacea/parasitology , Dogs/parasitology , Foxes/parasitology , Larva/classification , Larva/genetics , Molecular Sequence Data , Seals, Earless/parasitology , Sequence Alignment , Siberia/epidemiology , Swine/parasitology , Trichinella/classification , Trichinella/isolation & purification , Trichinellosis/parasitology , Ursidae/parasitology , Walruses/parasitologyABSTRACT
Sixty-three (24.3%) out of 259 residents of coastal settlements were found to have a positive immune response to Trichinella antigen. All the seropositive cases were represented by people belonging to three ethnic groups: Chukchi (97.8%), Eskimos (1.2%), and Yakuts (1.0%). The antibody titers varied from 1:100 (32.8%) to 1:1600 (8.7%). The highest titer reactivity was observed in marine mammal hunters, retired persons, and non-manual employees. There was a direct relationship between the antibody titer values and the dietary habits of the respondents preferring traditional foods prepared from marine mammal meat.
Subject(s)
Antibodies, Helminth/blood , Trichinella/immunology , Trichinellosis/ethnology , Trichinellosis/immunology , Adult , Animals , Arctic Regions , Caniformia/parasitology , Ethnicity , Feeding Behavior/ethnology , Female , Humans , Middle Aged , Siberia/epidemiology , Trichinellosis/epidemiology , Trichinellosis/parasitologyABSTRACT
The diagnostic efficiency of excretory-secretory antigens of Trichinella spiralis and Trichinella nativa with sera from Wistar rats experimentally infected with arctic Trichinella was comparatively tested. Trichinella derived from the muscles of wild carnivorous mammals inhabiting the biocenoses of Chukotka were used to infest animals and to obtain the antigen. There was a considerable excess of the effectiveness of enzyme immunoassay when the antigen derived from T. native larvae was used to analyze the titers of sera from the rats experimentally infected with arctic Trichinella.
Subject(s)
Antigens, Helminth , Helminth Proteins , Immunoglobulin G/blood , Trichinella spiralis , Animals , Antigens, Helminth/blood , Antigens, Helminth/immunology , Antigens, Helminth/isolation & purification , Cricetinae , Helminth Proteins/blood , Helminth Proteins/immunology , Humans , Immunoenzyme Techniques , Mesocricetus , Rats , Rats, Wistar , Trichinella spiralis/immunology , Trichinella spiralis/isolation & purificationABSTRACT
The 2010 seroepidemiological study of sera from 259 inhabitants of two settlements located on the shore of the Chukchi Sea revealed 63 (24.32%) seropositive respondents, which suggests the intensity of an epidemic process in these populated areas. The detection of specific antibodies in the population to the pathogen of trichinosis is of great importance for the objective assessment of the epidemic situation in the foci of invasion and the prediction of sporadically occurring epidemics of this anthropozoonosis.
