[Effect of detergent and glycerin on the efficacy of nasal immunotherapy with allergens and on the level of the lymphocyte subpopulation in the organs of local immunity]. / Vliianie detergenta i glitserina na éffektivnost' nazal'noi immunoterapii allergenami i uroven' subpopuliatsii limfotsitov v organakh mestnogo immuniteta.

Experimental mixed allergy to staphylococcal antigens in guinea pigs was treated by the intranasal administration of a staphylococcal allergen with a surfactant or glycerin added. The treatment was found to produce a hyposensitizing effect with respect to immediate and delayed hypersensitivity. The addition of glycerine enhanced this effect. At the same time the level of T-lymphocytes in the lungs and the lymph nodes of the respiratory tract returned to normal. Detergent used at a concentration of 2% abolished the hyposensitizing effect of the allergen, stimulated T-lymphocytes in the lymph nodes of the respiratory tract and the lungs; the number of T-suppressors decreased.

[Lymphocyte subpopulations in sensitization and specific immunotherapy in an experiment. Lymphocyte subpopulations in the specific immunotherapy of microbial allergy and subsequent heterologous pollen sensitization]. / Subpopuliatsii limfotsitov pri sensibilizatsii i spetsificheskoi immunoterapii v éksperimente. Subpopuliatsii limfotsitov pri spetsificheskoi immunoterapii mikrobnoi allergii i posleduiushchei geterologichnoi pyl'tsevoi sensibilizatsii.

The influence exerted by the specific immunotherapy (SIT) of delayed hypersensitivity (DH) to staphylococci and subsequent sensitization with tarragon pollen on the level of immunocompetent cells in the blood and lymphoid organs of guinea pigs was studied. On the whole, SIT normalized the characteristics of T- and B-lymphocytes, altered as the result of experimentally induced DH: the content of T-cells in the peripheral blood and the lymph nodes increased, while the number of B-cells in the blood and T gamma-suppressors increased. The subsequent heterologous sensitization with pollen abolished the effect of SIT, inducing the general decrease of the level of T gamma-lymphocytes and enhancing the number of T-lymphocytes in the lymph nodes.

[Formation of pollen-induced allergy in guinea pigs after specific immunotherapy with a bacterial allergen]. / Formirovanie pyl'tsevoi allergii u morskikh svinok posle spetsificheskoi immunoterapii bakterial'nym allergenom.

The investigation was aimed at elucidating the process of the development of allergy to exoallergens (e.g., to linear-leaf wormwood pollen) in the body after desensitization by specific immunotherapy (SIT) with heterologous (staphylococcal) allergen. The study revealed that in staphylococcal allergy the subsequent development of pollen sensitization occurred with greater intensity, and SIT was found to exert no influence on this process. For the first time pollen allergy was found to produce an unfavorable effect on the quality and effectiveness of SIT with heterologous staphylococcal allergen, this effect consisting in the acceleration and intensification of the process leading to the recovery of sensitization to staphylococci. The importance of measures for limiting contacts with heterologous allergens and their elimination during SIT is emphasized.

[Bronchospasm induction by mediators of antigen-specific stimulated lymphoid cells]. / Induktsiia bronkhospazma mediatorami antigenospetsificheski stimulirovannykh limfoidnykh kletok.

The action of lymphokines, soluble mediators of cell-mediated immunity was studied on isolated lungs of guinea-pigs. Antigen-specific antigens were shown to have bronchospastic activity as regards sensitized and nonsensitized lungs. The bronchospastic effect is not mediated by histamine or by other chemical bronchoconstrictors, and is not linked with activity of leukocyte migration inhibition factor.

[Comparative immunochemical and immunoallergic characteristics of staphylococci of different pathogenicity. II. Diagnostic value of extracts of Staphylococcus aureus and Staphylococcus epidermidis in delayed type allergic reactions]. / SRavitel'naia immunokhimicheskaia i immunoallergologicheskaia kharakteristika stafilokokkov razlichnoi patogennosti. Soobshchenie II. Diagnosticheskaia tsennost' razlichnykh ékstraktov Staphylococcus aureus i Staphylococcus epidermidis v allergicheskikh reaktsiiakh zamedlennogo tipa.

A total of 20 different allergens extracted from various staphylococcal strains were studied in the delayed hypersensitivity reactions in experiments on guinea-pigs. The most intensive dermatoallergic reactions were observed after the injection of the cytoplasmic fraction, acidic and alkaline extracts; reactions to the allergen of Ando-Verzhikovsky were less pronounced. The cytoplasmic fraction and the allergen of ando-Verzhikovsky were more active in the reaction of leukocyte migration inhibition in the blood. Both common and individual allergenic components were detected. No correlation between the results of the immunochemical and allergological studies of staphylococcal extracts was revealed.

[Physico-chemical properties of microbial antigens and the immuno-allergic tests]. / Fiziko-khimicheskie svoistva mikrobnykh antigenov i immuno-allergicheskie testy.

Experiments were conducted on guinea pigs sensitized by the delayed and immediate types of allergy. Different antigens obtained from the strain of Brucella abortus BA-19 were used for sensitization and the resolving action. Comparison of the resolving properties of the corpuscular, soluble (ultrasound treated) antigens and purified protein fractions, polysaccharide and RNA was carried out in the skin reactions of the immediate and delayed type, passive skin anaphylaxis, acute anaphylactic shock, and the Schults-Dale test. Immediate reactions to the purified protein fraction were weaker than those to the whole soluble antigen, by which the animals were sensitized. Polysaccharide and the RNA-fractions proved to be inactive in the allergic reactions.