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1.
Radiats Biol Radioecol ; 44(1): 5-14, 2004.
Article in Russian | MEDLINE | ID: mdl-15060932

ABSTRACT

The principles of information theory were applied for analysis of radiobiological effects. The perception of ionizing radiations as a signal enables living organism to discern their benefits or harm, to react to absolute and relatively small deviations, to keep the logic and chronicle of events, to use the former experience for reacting in presence, to forecast consequences. The systemic analysis of organism's response to ionizing radiations allows explaining the peculiarities of effects of different absorbed doses, hormesis, apoptosis, remote consequences and other post-radiation effects.


Subject(s)
Information Theory , Radiation, Ionizing , Radiobiology , Adaptation, Physiological , Algorithms , Animals , Apoptosis , Cells, Cultured/radiation effects , Cybernetics , Dose-Response Relationship, Radiation , Electromagnetic Fields , Humans , Radiation Dosage , Radiation Effects , Time Factors
2.
Radiats Biol Radioecol ; 40(2): 154-9, 2000.
Article in Russian | MEDLINE | ID: mdl-10819037

ABSTRACT

The fluorescence probe method was used for investigating the physical state of a total lipid phase of a bi-layer and an annular (near-protein) zone of the membrane lipids of lymphocytes in a peripheral blood of rats on the 10th day after a whole-body acute and chronic gamma-exposure to doses of 0.25, 0.5, and 1.0 Gy. It was discovered that exposure to doses of 0.25, 0.5, and 1.0 Gy revealed no reliable distinctions in the parameters of a physical state of the lipid component of membranes within the given period of observation if compared with those of controls. However chronic exposure to the same doses caused the increase in hydrophobicity of the total lipid phase of the membrane bi-layer with no change in the polarity of an annular lipid. The near-protein zone of lipids revealed a local decrease in microviscosity while the fluidity of total lipids of a membrane bi-layer remained unaltered. A detected change of tryptophan fluorescence of the membrane proteins after exposing them to small dosed has also been carried out.


Subject(s)
Cell Membrane/radiation effects , Lymphocytes/radiation effects , Lymphocytes/ultrastructure , Membrane Lipids/radiation effects , Animals , Cell Membrane/chemistry , Dose-Response Relationship, Radiation , Gamma Rays , Lymphocytes/chemistry , Male , Membrane Fluidity/radiation effects , Membrane Lipids/chemistry , Rats , Whole-Body Irradiation
3.
Aviakosm Ekolog Med ; 33(4): 43-7, 1999.
Article in Russian | MEDLINE | ID: mdl-10530385

ABSTRACT

Nonstimulated and isoproterenol, GTF, GITF, NaF stimulated activities of the adenylate cyclase in sarcolemma in white rats' myocardium was studied after two weeks of hypokinesia. As was established, in restrained animals the sensitivity of adenylate cyclase to the specified agents was increased and transition to the bimodal GTF regulation took place. It is hypothesised that involvement of membrane-bound Gi-proteins in the adrenergic effects on cardiomyocytes is one of mechanisms of the cardiotropic effects of restraint and heart distresses.


Subject(s)
Adenylyl Cyclases/metabolism , Hypokinesia/enzymology , Myocardium/enzymology , Animals , Cell Membrane/enzymology , GTP-Binding Protein alpha Subunits, Gi-Go/metabolism , Guanylate Cyclase/metabolism , Male , Rats , Sarcolemma/enzymology
4.
Radiats Biol Radioecol ; 34(4-5): 651-6, 1994.
Article in Russian | MEDLINE | ID: mdl-7951898

ABSTRACT

Incorporated 137Cs (absorbed dose of 0.26 Gy) causes decrease of myocardial's contractile function and inotropic response to beta-adrenagonists effect, isoproterenol-stimulated adenylate cyclase activity and beta-adrenoreceptors affinity. Adrenergic effects, mediated by alpha-adrenergic structures on heart contractile function, on the contrary, become stronger, that is due to the increase of the receptors' density on sarcolemma surface.


Subject(s)
Cesium Radioisotopes , Myocardial Contraction/radiation effects , Receptors, Adrenergic, alpha/radiation effects , Receptors, Adrenergic, beta/radiation effects , Adenylyl Cyclases/metabolism , Animals , Enzyme Activation/radiation effects , Myocardial Contraction/physiology , Myocardium/enzymology , Rats , Receptors, Adrenergic, alpha/physiology , Receptors, Adrenergic, beta/physiology , Time Factors
5.
Biofizika ; 32(3): 438-42, 1987.
Article in Russian | MEDLINE | ID: mdl-3040128

ABSTRACT

Cardiomyocyte plasmalemma membranes of adult (12 months) and old (24-26 months) rats have been studied. Ageing is accompanied with an essential decrease of the content of the membrane protein SH-groups. We have found an increase in the fraction of strongly immobilized spin-labeled SH-groups, a decrease in that of unsaturated lipids, as well as an increase in that of lysophosphatidylcholine in old rats membranes. The pattern of the response of beta-adrenergic receptors to isoproterenol is changed with age. The mobility of spin probe 5NS in the membranes of old rats is lowered at 22.5 degrees C. However there is no statistically significant age difference in the spin-probe mobility at 37 degrees C. Possible role of lysophosphatidylcholine in the stabilization of membrane lipid "fluidity" at physiological temperatures is suggested.


Subject(s)
Aging/metabolism , Myocardium/metabolism , Sarcolemma/metabolism , Animals , Cell Membrane/metabolism , Electron Spin Resonance Spectroscopy , Fatty Acids/metabolism , Male , Membrane Lipids/metabolism , Membrane Proteins/metabolism , Phospholipids/metabolism , Rats , Rats, Inbred Strains , Sulfhydryl Compounds/metabolism
6.
Biofizika ; 29(4): 640-2, 1984.
Article in Russian | MEDLINE | ID: mdl-6091786

ABSTRACT

Below 37 degrees the mobility of lipids in rat brain synaptic membranes evaluated by the spin probe method is higher in old (24-26 months) than in young (5-6 months) animals. The number of ouabain receptors in membranes increases with age, while maximum rate and Km values are not changed. The relation between structural state of membrane and properties of ouabain binding centers is discussed.


Subject(s)
Aging , Brain/metabolism , Synaptic Membranes/metabolism , Animals , Binding Sites , Brain/enzymology , Cell Membrane/metabolism , Lipid Bilayers/metabolism , Rats , Receptors, Drug/metabolism , Sodium-Potassium-Exchanging ATPase/metabolism , Spin Labels , Synaptic Membranes/enzymology , Temperature
7.
Biofizika ; 27(4): 650-2, 1982.
Article in Russian | MEDLINE | ID: mdl-6289920

ABSTRACT

In the brain synaptic membranes from old rats (24-26 months) in comparison with the mature ones (6-7 months) an increase was shown in disintegration rate with DDS Na, mobility of 5-, 16-doxyl-stearate spin probes and in thermosensitivity of acetylcholinesterase. The total number of SH-groups decreased. While protein composition remained constant lysophosphatidylcholine and saturated fatty acid content in old animals increased. The age-dependent structural rearrangement of synaptic membranes is suggested.


Subject(s)
Brain/growth & development , Synaptic Membranes/physiology , Aging , Animals , Electron Spin Resonance Spectroscopy , Rats , Sodium Dodecyl Sulfate , Temperature
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