[Effect of Glycine max lectin on the interaction of prothrombin and erythrocytes]. / Vliianie lektina Clycine max na vzaimodeistvie protrombina s éritrotsitami.

Native porcine erythrocytes do not initiate blood coagulation, though even the weak association (Kd = 4,25 +/- 9,35 microM) of prothrombin with their surface which is limited to the projection of two phospholipid polar head groups onto the external cell membrane, exerts a slight but authentic influence on the acceleration of blood clotting. There are 11.9 x 10(7) sites for 125I-prothrombin on one erythrocytes. At physiological concentration of prothrombin the surface of erythrocytes is far from saturation. The binding of 125I-labelled prothrombin to erythrocytes after their surface was covered by lectin Glycine max by means of the accessible residues of N-acetyl-D-galactosamine and D-galactose, glycoproteins and dlycolipids can never become saturated with any used concentration of the ligand. In the presence of the suspension of erythrocytes treated lectin platelet free plasma coagulates at the same speed as the control plasma.

[Advantages of using pharmacologic tests to characterize bacterial vaccines]. / O tselesoobranznosti ispol'zovaniia farmakologicheskikh testov dlia kharakteristiki bakterial'nykh vaktsin.

The possibility of using pharmacological tests to determine side effects produced by adsorbed DPT vaccine and chemical typhoid vaccine was studied. Both vaccines were shown to be capable of prolonging sleep induced by hexenal or thiopental in experimental animals. The following difference in the action of these two preparations was revealed on the model of "chemical convulsions" induced by the injection of thiosemicarbazide: while immunization with DPT vaccine acitivated thiosemicarbazide-induced convulsive syndrome in mice, no such stimulating effect was observed after the injection of typhoid, vaccine. The study of pharmacological effects allowed to evaluate some aspects of the side effects producted by prophylactic preparations.