ABSTRACT
AIM: Study adhesive activity of enterobacteria in the model of hemagglutination reaction with animal and avian erythrocytes and clarify structures responsible for adhesion in enterobacteria. MATERIALS AND METHODS: 58 cultures of enterobacteria were used, of which: 21 Escherichia coli strains, 13 Citrobacter spp., 11 Morganella morganii, 9 Proteus spp., 4 Hafnia alvei. Erythrocytes of various animals and birds were used in hemagglutination reaction. Electron-microscopical studies were carried out in JEM-100B (Japan) electron microscope. RESULTS: Use of avian erythrocytes as a target of adhesion determination, compared with animal erythrocytes, has shown that bacteria can cause D-mannose-sensitive hemagglutination reaction, linked with the presence of 110 - 420 nm long and 5.0 - 5.4 nm wide cilia in the microorganisms. CONCLUSION: Adhesion of enterobacteria was shown to be a complex process, depending on the presence of certain fimbrial structures, use of those results in specific interaction of the microbe with certain host cell receptors. Avian erythrocytes are a model target cells.
Subject(s)
Bacterial Adhesion/immunology , Enterobacteriaceae/pathogenicity , Erythrocytes/microbiology , Hemagglutination/immunology , Animals , Birds/microbiology , Enterobacteriaceae/immunology , Erythrocytes/pathology , Erythrocytes/ultrastructure , Fimbriae, Bacterial/immunology , Fimbriae, Bacterial/ultrastructure , Hemagglutination Tests , HumansABSTRACT
The comparative study of adhesive, hemolytic, DNA-ase, lecithinase, antilysozymic, anticomplementary activities of mono- and associated cultures of 57 Enterobacter spp., 61 Citrobacter spp. and 55 Serratia spp. strains, isolated from patients with pyoinflammatory, intestinal and urological diseases is carried out. Different variations of cocultivated bacteria including Enterobacter and Citrobacter, Enterobacter and Serratia, Citrobacter and Serratia are used. It was shown, that cocultivated Enterobacter, Citrobacter and Serratia bacteria increased the persistent properties of mixt cultures.
Subject(s)
Citrobacter/physiology , Enterobacter/physiology , Enterobacteriaceae Infections/microbiology , Serratia/physiology , Animals , Bacterial Adhesion/physiology , Citrobacter/pathogenicity , Complement Inactivator Proteins/metabolism , Deoxyribonucleases/metabolism , Enterobacter/pathogenicity , Enterobacteriaceae Infections/complications , Enterobacteriaceae Infections/pathology , Enterocolitis/microbiology , Hemolysin Proteins/metabolism , Humans , Inflammation/pathology , Muramidase/antagonists & inhibitors , Muramidase/metabolism , Phospholipases/metabolism , Serratia/pathogenicity , Symbiosis , Urinary Tract Infections/microbiology , Urinary Tract Infections/pathologyABSTRACT
Data on the apoptosis phenomenon with enterobacteria used as a model are presented. One of the mechanisms regulating the vital activity of eukaryotic cells is, together with cell proliferation and differentiation, the phenomenon known as "apoptosis". This physiological process of the eukaryotic cells death is used by many parasites in parasite--host relationships in different epitopes. The system known to trigger programmed cell death, is the surface receptor Fas, the receptor of tumor necrosis factor (TNF alpha) activated by the corresponding FasL ligand and TNF alpha, which further triggers the cascade mechanisms of the execution program. In various representatives of enterobateria different proteins serve as Fas ligand, viz. protein IpaB in Shigella flexneri, SipB activating converting enzyme IL-1 beta, identical to capsase-1, in Salmonella spp., YopP in Yersinia spp. Still the mechanism triggering apoptosis in Yersinia spp. has some original features. In Escherichia coli alpha-hemolysin is the factor triggering the suicidal program, the triggering mechanism being mediated by an increase in intracellular calcium ions.
