Beekeeping breakthrough: unveiling hive health with a portable membrane inlet mass spectrometry detection method.

Supporting bee populations is essential considering threats posed by human activities like pesticide usage and habitat destruction. However, the current methods for monitoring and analyzing beehives and their surrounding environments are invasive, complex, and time-consuming. These methods often rely heavily on laboratory analyses, making them difficult to implement independently in the field. This study explores the application of portable membrane inlet mass spectrometer (MIMS) for noninvasive hive analysis, demonstrating its ability to detect various compounds indicative of hive conditions and environmental stressors. In addition to the expected compounds found in beehives, such as α-bergamotene, hexadecanoic acid, heptadecane, hexadecanamide, α-bisabolol-, 9-octadecenamide, (Z) - , and benzaldehyde, unexpected compounds, pollutants, like indane (polycyclic aromatic hydrocarbon) and carbofuran (pesticide), were also detected. The MIMS detection method provides rapid, accurate, and real-time results, making it suitable for preventive measures against bee diseases and integral to environmental biomonitoring. This integration of technology represents a significant advancement in bee conservation efforts, offering hope for the future of both bees and ecosystems.

Contamination levels, influencing factors, and risk assessment of polybrominated diphenyl ethers in house dust of northern Serbia.

Polybrominated diphenyl ethers (PBDEs) are a group of compounds that, due to their applications, are considered mainly indoor contaminants. To obtain the first information about the presence of PBDEs in Serbia, dust samples (n = 50) were collected in settlements in the northern Serbian province of Vojvodina. The selected/target congeners (BDE-28, 47, 99, 100, 153, 154, and 183) were extracted from house dust by microwave-assisted extraction technique, and purified extracts were analyzed on a dual-column gas chromatograph with micro-electron capture detectors. A wide range of ΣPBDEs was detected (0.295 to 394 ng g-1 dust), which reflects large differences in contamination among the examined homes. For the majority of samples (72%), ΣPBDEs were lower than 5 ng g-1 indicating that people living in Vojvodina province are exposed to low concentrations of PBDEs present in their households. Based on principal component analysis (PCA), balcony areas and age of the house positively correlate with the PBDE congeners with higher detection frequencies (≥ 50%), namely, with BDE-99, BDE-153, and BDE-183. Statistically significant positive correlation (p < 0.01) was obtained for BDE-99 and the number of household's members. Estimated daily intakes (EDItot) were calculated for ingestion and dermal absorption of dust for two age groups-adults and toddlers. These are the first data on PBDE status in the area of the Western Balkan, and the health risk assessment indicates that PBDE levels obtained in household dust do not pose a risk for human health.

Heavy elements in indoor dust from Serbian households: pollution status, sources, and potential health risks.

Occurrence of five heavy elements (HEs) was analyzed in indoor dust (n = 45) in households from four settlements in Vojvodina Province, Serbia. Overall medians (mg/kg) of Pb (5.6), Ni (5.2), Cu (27), and Cr (6.8) were below soil background values, while median for Cd (1.1) exceeded it. Pollution load index showed that the households' microenvironment in examined region might be regarded as unpolluted. Integrated pollution index revealed low contamination by Pb, Ni, and Cr, but high with Cu and Cd. Source apportionment by principal component analysis and positive matrix factorization suggested two possible sources: outdoor pollution and household materials. Hazard index was below safe limit (<1), indicating no adverse non-carcinogenic health effects. Estimated total carcinogenic risk for children and adult population was not negligible. Results indicated that attention should be paid to the presence of HE in indoors to conduct effective control measures and to ensure the health of the population.

A case study on the occurrence of polycyclic aromatic hydrocarbons in indoor dust of Serbian households: Distribution, source apportionment and health risk assessment.

This study was conducted in order to obtain the first insight into the occurrence, potential sources, and health risks of polycyclic aromatic hydrocarbons (PAHs) in indoor dust. Samples (n = 47) were collected from households in four settlements in the northern Serbian province of Vojvodina. Total concentrations of 16 EPA priority PAHs in the dust samples varied from 140 to 8265 µg kg-1. Mean and median values for all samples were 1825 and 1404 µg kg-1, respectively. According to the international guidelines for indoor environment, PAH content can be regarded as normal (<500 µg kg-1) for ∼6% of the samples, high (500-5000 µg kg-1) for ∼87% of the samples, and very high (5000-50000 µg kg1) for ∼6% of the samples. In all settlements, PAHs with 4 rings were the most prevalent (accounting for 40-53% of the total PAHs). They were followed by 3-ringed PAHs (29-40%), which indicates rather uniform PAH profiles in the analyzed dust. Based on diagnostic ratios, principal component analysis (PCA), and positive matrix factorization (PMF), pyrogenic sources, such as vehicle emissions and wood combustion were the dominant sources of PAHs in analyzed samples. Health risk assessment, which included incidental ingesting, inhaling and skin contact with PAHs in the analyzed dust, was evaluated by using the incremental lifetime cancer risk (ILCR) model. Median total ILCR was 3.88E-04 for children, and 3.73E-04 for adults. Results revealed that major contribution to quite high total ILCRs was brought by dermal contact and ingestion. Total cancer risk for indoor dust indicated that 85% of the studied locations exceeded 10-4. This implies risk of high concern, with potential adverse health effects. The results are valuable for future observation of PAHs in indoor environment. They are also useful for regional authorities who can use them to create policies which control sources of pollution.

Comprehensive assessment of heavy elements and evaluation of potential human health risk in the urban environment: a case study from Novi Sad, Serbia.

This study aimed to determine seasonal (summer vs. winter) and spatial distribution of the selected heavy elements (HEs) (As, Cd, Co, Cr, Cu, Ni, and Pb) in soil samples collected from a typical Central European town, Novi Sad, Serbia. The highest Pb concentrations were observed in summer because of intensive ground-flushing, whereas Cu had its highest concentration in winter, which may be attributed to traffic emissions. Source characterization and spatial distribution were carried out using cluster analysis (CA), principal component analysis (PCA), variogram calculation and theoretical model fitting, GIS-based geostatistical methods, and positive matrix factorization (PMF) data processing tools. Environmental impact of HEs found in different functional areas demonstrated that the quantified concentrations of Pb, As, Co, and Cu ranked soil as unpolluted to moderately polluted, while the presence of Co, Ni, and Cr classified urban soil as moderately polluted. Pollution load index (PLI) suggested a significant HEs enrichment while the new modified approach to Nemerow integrated risk index (NIRI) indicated high risk, being extreme for some selected locations. Machine learning classifiers were used for the first time to identify the differences between urban soil and dust samples in situations when simultaneous analysis of both matrices was carried out, as well as for temporal distribution (summer versus winter), based on the obtained concentration of HEs. Variogram calculation suggested that the pattern in spatial variability within the system emerged from the combined action of key structural factors (e.g., the parent soil material, landforms and topography, and climate) and random factors related to human activities. The estimated human health risk for two segments of the population revealed that ingestion is the primary route of exposure to HEs for children and adults.

Pollution status and health risk caused by heavy elements in the flooded soil and vegetables from typical agricultural region in Vojvodina Province, Serbia.

The investigation conducted in the Vojvodina Province, as a typical European and one of the biggest agricultural regions in the Balkans, offers the research methodology that could be used for any non/flooded agricultural region. The flood impact on heavy elements (HE) content in the flooded arable soil (n = 16) in relation to the control soil (n = 16) was examined, as well as their accumulation in the most often cultivated vegetables (n = 96) in the studied area. Results revealed that the flood did not significantly change the pseudo total HEs concentration in the soil as well as their amounts accumulated in different soil fractions. In both soils, only the average content of Ni exceeded the maximum permissible values set by Serbian soil quality standard which is in line with the Dutch standard. In comparison with the background values, notable enrichment is found for most of analyzed elements in both soils. Soil pollution status was assessed through several indices indicating that contamination range was in the domain from a moderate to a highly polluted. The principal component analysis demonstrated that soil contamination was probably originated from agricultural/anthropogenic activities (Cd, Cu, As, Pb), apart from Ni, Cr, and Co which came from natural weathering of the parent material. Carcinogenic and non-carcinogenic risks of selected HEs for Serbian population in the investigated region were below the threshold values. The average levels of Pb in investigated potato and carrot samples were higher than the maximum allowable concentrations established by EU/Serbian regulation. The total hazard quotients (THQ) of HEs through intake of analyzed vegetables were below the safe threshold (˂ 1), suggesting the absence of adverse health effects.

Occurrence of heavy elements in street dust from sub/urban zone of Tianjin: pollution characteristics and health risk assessment.

Main purpose of this study was to determine the concentrations of selected heavy elements (As, Cd, Pb, Cu, Co, Cr and Ni) in the street dust samples (n = 49) collected from seven districts located in suburban/urban zone of Tianjin in order to estimate their possible sources and degree of environmental pollution as well as human health risk. Mean concentrations (mg kg-1) of As (19.3), Cd (0.60), Pb (28.4) and Cu (62.7) were above their corresponding soil background values. According to the results of multivariate statistical analysis, the accumulation of As, Cd, Pb, Cu and Cr in street dust was affected by anthropogenic activities, while the contents of Ni and Co were associated with natural sources. Pollution degree by geo-accumulation index had the following trend: Cd > Cu > As > Pb > Cr > Ni > Co. Dust contamination with Cd ranged from unpolluted to highly polluted. Potential ecological risk indicated low (Pb, Cu, Cr, Co and Ni) to high (Cd) risk, while potential risk index showed moderate and very high risks. Non-carcinogenic risk of the studied elements was below safe level (<1). Data obtained in this investigation gave the additional values to the knowledge needed for future monitoring and risk assessment, relating the presence of heavy elements studied in suburban/urban areas.

Seasonal, spatial variations and risk assessment of heavy elements in street dust from Novi Sad, Serbia.

This is the first study carried out to determine the levels, spatial and temporal variations and risk indices of street dust in Serbia. Sixty street dust samples representing four functional areas: school, recreational, residential and industrial were collected from Novi Sad during summer and winter. The purpose was to obtain the concentration of seven heavy elements, evaluate the possible sources and degree of environmental pollution and human health risks. The average concentration of As, Cd, Co, Cr, Cu, Ni and Pb (mg/kg) during winter was: 7.96, 0.40, 7.73, 12.1, 57.6, 14.0 and 70.6, while in summer it was: 1.88, 0.54, 4.31, 60.1, 42.7, 28.1, and 62.5, respectively. Interpolation method together with the correlation matrix and the results of multivariate statistics supported anthropogenic origin of Pb, Cr and Cu, natural origin of Ni, Cd and As and mix origin of Co. Pollution indices such as geoaccumulation index (Igeo) indicated existence of unpolluted to extreme risk regarding to environmental pollution. Moreover, the potential pollution index (Er) showed low (Pb) to moderate (Ni) risk while potential risk index (RI) and modified potential risk index (mRI) indicated considerable and very high risks. Health risk assessment showed no non-carcinogenic risk of heavy elements for adults and children.