ABSTRACT
The distribution of IgA subclasses in IgA immune complexes (IgA IC) in sera of patients with primary IgA glomerulonephritis and Henoch-Schönlein purpura nephritis was analysed. High levels of IgA IC containing both IgA1 and IgA2 subclasses were present in correlation with the phases of clinical activity. In these nephropathies the finding of IgA subclass distribution in IgA IC similar to that found in secretions may add further support to the hypothesis that IgA IC are of mucosal origin, albeit a primary derangement of the humoral immune system in these patients cannot be disregarded.
Subject(s)
Antigen-Antibody Complex/immunology , Glomerulonephritis/immunology , IgA Vasculitis/immunology , Immunoglobulin A/immunology , Adolescent , Adult , Aged , Antigen-Antibody Complex/analysis , Child , Child, Preschool , Female , Humans , Immunoglobulin A/classification , Male , Middle AgedSubject(s)
Antigen-Antibody Complex , Glomerulonephritis/drug therapy , Immunoglobulin A , Phenytoin/therapeutic use , Adult , Humans , MaleABSTRACT
IgA subclasses in circulating immune complexes (IgA1IC), serum immunoglobulins and mesangial deposits in Berger's and Schönlein-Henoch glomerulonephritis (GN) were studied. Both IgA1IC and IgA2IC were significantly higher in Berger's and Schönlein-Henoch GN than in healthy people. In phases of clinical activity both IgAIC subclasses further increased. The IgA1/IgA2 ratio was found not to differ from controls in either groups of patients. An increase in polymeric IgA was observed in Berger's and in Schönlein-Henoch GN. Both IgA subclasses were found in mesangial deposits.
Subject(s)
Antigen-Antibody Complex/analysis , Glomerulonephritis/immunology , IgA Vasculitis/immunology , Immunoglobulin A/analysis , Kidney/immunology , Biopsy , Fluorescent Antibody Technique , Glomerulonephritis/etiology , Humans , Immunoglobulin A/classificationABSTRACT
In some renal, gastroenterological and dermatological diseases the pathogenic role of circulating immune complexes (CIC) of IgA type has been defined. The methods so far proposed to investigate CIC are for the most part able to identify IgGIC, while only few can do the same for IgAIC. The Authors discuss in details the technique and the sensibility and specificity of a new test for IgAIC based on the linkage with the conglutinin in solid phase and propose this test for the clinical study of IgA diseases.
Subject(s)
Antigen-Antibody Complex/analysis , Complement Fixation Tests , Immunoglobulin A/analysis , Antigen-Antibody Complex/isolation & purification , Humans , Immunoglobulin A/isolation & purification , Methods , Reference ValuesABSTRACT
The application of tests for the determination of serum immune complexes in nephrology has supplied fresh pathogenetic and symptomatological information. An account is given of results obtained in primary and secondary glomerulonephritis using four methods; the solid-phase Clq test, the polyethylene glycol precipitation test, the immunofluorescence on polymorphonucleates tests, and the solid-phase conglutinins test (with anti-IgA antibodies). The results take on a symptomatological meaning in many classes of human glomerulonephritis, both in the differentiation of primary forms and those secondary to systemic diseases, and in prognosis. A critical review is made of the data obtained in a personal series in the light of a long-term follow-up. The limits and specificity of each test are also discussed.
