1.
Curr Protoc Protein Sci
; Chapter 10: Unit 10.11, 2001 May.
Article
in English
| MEDLINE
| ID: mdl-18429093
ABSTRACT
This overview provides a thorough discussion of the theory and application of autoradiography for determining the spatial distribution of radioisotope-labeled substances within a membrane or specimen material. The various types of film are discussed along with instructions on their correct handling. Quantitative autoradiography is described as a means of calibrating experimental results. Importantly, specific guidelines are given for pre-flashing film, along with exposure and processing procedures.