ABSTRACT
The extracts of saponified cereal fibers of whole grains of corn (Zea mays cv. microsperma KOERN.), wheat (Triticum aestivum L.), spelt (Triticum spelta L.), and rice (Oryza sativa L.) were investigated for dehydrodimers of ferulic acid using gas-liquid chromatography (GLC) with mass spectrometric detection (GLC-MS) and flame ionization detection (GLC-FID). In addition to the 8,5'-, 8, 8'-, 5,5'-, and 8-O-4'-coupled diferulic acids previously identified from other plant materials the 4-O-5'-coupled diferulic acid (E)-3-[4-[(E)-2-carboxyvinyl]-2-methoxyphenoxy]-4-hydroxy-5-methoxyci nnamic acid (4-O-5'-DFA) was identified in all fibers investigated. This new diferulate was authenticated by comparison of its mass spectrum and its relative GLC retention time with those of the synthesized compound. Semiquantitative determination of 4-O-5'-DFA showed that it is present at 8-30 microg/g, approximately 70-100 times lower concentrations than the sum of 8,5'-coupled diferulic acids, the major diferulic acids in the investigated fibers.
Subject(s)
Coumaric Acids/analysis , Dietary Fiber/analysis , Edible Grain/chemistry , Mass SpectrometryABSTRACT
Inverse associations were demonstrated between DMFT and total calcium, phosphorus, magnesium, strontium, potassium, and fluoride concentrations in individual plaques. Lithium, strontium, fluoride, and zinc contents were inversely related to plaque mass. Consistent interdependence patterns between elements indicate the operation of a mineral level regulating mechanism, effective under diverse environmental conditions.
Subject(s)
DMF Index , Dental Plaque/analysis , Minerals/analysis , Adolescent , Age Factors , Australia , Calcium/analysis , Child , Copper/analysis , Female , Fluorides/analysis , Humans , Lead/analysis , Lithium/analysis , Magnesium/analysis , Male , Phosphorus/analysis , Potassium/analysis , Sex Factors , Strontium/analysis , Trace Elements , Zinc/analysisSubject(s)
Dental Plaque/analysis , Minerals/analysis , Adolescent , Age Factors , Australia , Calcium/analysis , Child , Copper/analysis , Female , Humans , Lead/analysis , Lithium/analysis , Magnesium/analysis , Male , Phosphorus/analysis , Potassium/analysis , Sex Factors , Strontium/analysis , Zinc/analysisABSTRACT
Individual caries experience (DMFT) and the total fluoride content of dental plaque were determined for 72 schoolchildren, aged 9.7-13.0 years, lifelong residents in one of three New South Wales towns, where the fluoride levels of the reticulated water supplies were: Katoomba, less than 0.1 parts/10(6); Sydney, 1.0 parts/10(6) for 4 years; and Yass, 1.0 parts/10(6) for 16 years, prior to sampling. The mean fluoride content of plaque in Sydney (22.6, s.d. = +/- 16.8 parts/10(6)) and Yass (25.6, s.d. = +/- 16.4 parts/10(6)) differed significantly (t = 2.27, P less than 0.05 and t = 3.30, P less than 0.02, respectively) from that in Katoomba (13.5, s.d. = +/- 8.3 parts/10(6)). Significant inverse associations were demonstrated between total plaque fluoride and individual caries experience (DMFT) in Sydney (r = -0.45, P less than 0.025) and overall (r = -0.28, P less than 0.010). Inverse trends were established between plaque quantity (dry weight of plaque collected) and fluoride levels. No associations could be demonstrated between fluoride treatment (dentifrice, tablets or topical application) and plaque fluoride, DMFT or plaque quantity.