Subject(s)
Armadillos/physiology , Reproduction , Xenarthra/physiology , Animals , Female , Pregnancy , Seasons , Stress, Physiological/physiopathology , Time FactorsSubject(s)
Animal Population Groups , Animals, Wild , Armadillos , Leprosy/veterinary , Xenarthra , Animals , MexicoABSTRACT
Impact resistant polyethylene cages were evaluated in these laboratories as replacements for plywood boxes for housing nine-banded armadillos. They were found to be less expensive, easier to sanitize, and to require less than one-third as much floor space per animal as the boxes. This innovation has made it practical to house large numbers of armadillos economically and conveniently in almost any animal facility.
Subject(s)
Armadillos , Housing, Animal , Xenarthra , Animals , Animals, LaboratoryABSTRACT
Extraction and cleanup methods used for the analysis of organophosphate pesticides are similar to those used for chlorinated hydrocarbons but differ in some important details. Many organophosphates are lost during extraction of acetonitrile solutions containing organophosphates with petroleum ether; dichloromethane is substituted. Also, cleanup by column chromatography on mixed adsorbents containing charcoal results in better recoveries than can be obtained on Florisil alone. The detectors used for measurement of organophosphates by GLC are almost unique for this group of compounds. TLC is very useful for confirming the identities of the compounds, and several promising optical methods are available for their measurement. Methods for the analysis of metabolites of this group are basically similar, since all organophosphates undergo dealkylation reactions and cleavage of the acid anhydride bond. However, measurement of the polar group which is removed during phosphorylation or other reactions involving cleavage of the acid anhydride bond is a problem unique for each compound.
Subject(s)
Insecticides/analysis , Organophosphorus Compounds , Organothiophosphorus Compounds , Charcoal , Chemistry, Organic/methods , Chromatography , Chromatography, Gas/instrumentation , Chromatography, Gas/methods , Chromatography, Gel , Chromatography, Thin Layer/methods , Edible Grain/analysis , Food Contamination/analysis , Insecticides/metabolism , Magnesium , Magnesium Silicates , Methods , Plants/analysis , Silicon Dioxide , Solubility , Water Pollutants, Chemical/analysisSubject(s)
Carcinogens/toxicity , Pesticides/toxicity , Animals , Female , Male , Mice , Mice, Inbred Strains , Rats , Time FactorsABSTRACT
Eight of twenty armadillos (Dasypus novemcinctus L.) developed severe lepromatous leprosy 3 to 3.5 years after inoculation with viable Mycobacterium leprae. A total of 988 grams of lepromas containing an estimated 15 to 20 grams of leprosy bacilli has been harvested from these animals. The large amounts of material now available will permit in-depth studies of the biochemistry and metabolism of the leprosy bacillus, and the animal model should make possible definitive studies on the immunology, chemotherapy, and epidemiology of the disease.
