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1.
Sci Rep ; 12(1): 17641, 2022 Oct 21.
Article in English | MEDLINE | ID: mdl-36271240

ABSTRACT

A direct quantization of the Newtonian interaction between two masses is known to establish entanglement, which if detected would witness the quantum nature of the gravitational field. Gravitational interaction is yet compatible also with gravitational decoherence models relying on classical channels, hence unable to create entanglement. Here, we show in paradigmatic cases that, despite the absence of entanglement, a classical-channel model of gravity can still establish quantum correlations in the form of quantum discord between two masses. This is demonstrated for the Kafri-Taylor-Milburn (KTM) model and a recently proposed dissipative extension of this. In both cases, starting from an uncorrelated state, a significant amount of discord is generally created. This eventually decays in the KTM model, while it converges to a small stationary value in its dissipative extension. We also find that initial local squeezing on the state of the masses can significanlty enhance the generated discord.

2.
J Mol Biol ; 318(2): 333-49, 2002 Apr 26.
Article in English | MEDLINE | ID: mdl-12051841

ABSTRACT

We have analyzed in detail the structure of RAP1-UAS(RPG) complexes in Saccharomyces cerevisiae cells using multi-hit KMnO(4), UV and micrococcal nuclease high-resolution footprinting. Three copies of the Rap1 protein are bound to the promoter simultaneously in exponentially growing cells, as shown by KMnO(4) multi-hit footprinting analysis, causing extended and diagnostic changes in the DNA structure of the region containing the UAS(RPG). Amino acid starvation does not cause loss of Rap1p from the complex; however, in vivo UV-footprinting reveals the occurrence of structural modifications of the complex. Moreover, low-resolution micrococcal nuclease digestion shows that the chromatin of the entire region is devoid of positioned nucleosomes but is susceptible to changes in accessibility to the nuclease upon amino acid starvation. The implications of these results for the mechanism of Rap1p action are discussed.


Subject(s)
DNA, Fungal/genetics , DNA, Fungal/metabolism , DNA-Binding Proteins/metabolism , Saccharomyces cerevisiae Proteins/metabolism , Saccharomyces cerevisiae/genetics , Saccharomyces cerevisiae/metabolism , rap1 GTP-Binding Proteins/metabolism , Amino Acids/metabolism , Base Sequence , Chromatin/genetics , DNA Footprinting , DNA, Fungal/chemistry , DNA-Binding Proteins/genetics , Kinetics , Macromolecular Substances , Micrococcal Nuclease , Models, Molecular , Molecular Sequence Data , Peptide Elongation Factor 1 , Peptide Elongation Factors , Promoter Regions, Genetic , Saccharomyces cerevisiae Proteins/genetics , Transcription, Genetic , rap1 GTP-Binding Proteins/chemistry
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