Subject(s)
Chromosome Mapping , Hybrid Cells , Animals , Cricetinae , Electrophoresis , Gene Frequency , Humans , Hybrid Cells/enzymology , Hybrid Cells/ultrastructure , MiceABSTRACT
Regional localization studies of genes coding for human PGD, PPH1, PGM1, UGPP, GuK1, Pep-C, and FH, which have been assigned to chromosome 1, were performed with man-Chinese hamster somatic cell hybrids, Informative hybrids that retained fragments of the human chromosome 1 were produced by fusion of hamster cells with human cells carrying reciprocal translocations involving chromosome 1. Analysis of the hybrids that retained one of the translocation chromosomes or de novo rearrangements involving the human 1 revealed the following gene positions: PGD and PPH1 in 1pter leads to 1p32, PGM1 in 1p32 leads to 1p22, UGPP and GuK1 in 1q21 leads to 1q42, FH in 1qter leads to 1q42, and Pep-C probably in 1q42.
Subject(s)
Chromosome Aberrations , Chromosomes, Human, 1-3 , Genes , Hybrid Cells , Translocation, Genetic , Animals , Cell Line , Chromosome Mapping , Cricetinae , Endopeptidases/metabolism , Fumarate Hydratase/metabolism , Guanylate Cyclase/metabolism , Humans , Hybrid Cells/enzymology , Phosphoglucomutase/metabolism , Phosphogluconate Dehydrogenase/metabolism , Phosphopyruvate Hydratase/metabolism , UTP-Glucose-1-Phosphate Uridylyltransferase/metabolismABSTRACT
The position of genes coding for PGD, PPH1, UGPP, GuK1, PGM1, Pep-C, and FH on human chromosome 1 was investigated by analysis of karyotype and enzyme phenotypes in man-Chinese hamster somatic cell hybrids carrying aberrations involving chromosome 1. Suitable hybrid cell lines were obtained by X-irradiation of hybrid cells carrying an intact chromosome 1 and by fusion of human cells from a clonal population carrying a translocation involving chromosome 1 with Chinese hamster cells. The latter human cell population had been isolated following X-irradiation of primary Lesch-Nyhan fibroblasts. In addition, products of de novo chromosome breakage in the investigated hybrid lines were utilized. By integrating the results of these analyses with earlier findings in our laboratory, the following positions of genes are deduced: PGD and PPH1 in 1p36 leads to 1p34; PGM1 in 1p32; UGPP in 1q21 leads to 1q23; GuK1 in 1q31 leads to 1q42; Pep-C in 1q42; and FH in 1qter leads to 1q42.
Subject(s)
Chromosome Aberrations , Chromosomes, Human, 1-3 , Genes , Hybrid Cells/radiation effects , Animals , Cell Line , Chromosome Mapping , Cricetinae , Endopeptidases/metabolism , Fumarate Hydratase/metabolism , Humans , Hybrid Cells/enzymology , Male , Peptide Hydrolases/metabolism , Phenotype , Phosphoglucomutase/metabolism , Phosphogluconate Dehydrogenase/metabolism , Phosphotransferases/metabolism , UTP-Glucose-1-Phosphate Uridylyltransferase/metabolismSubject(s)
Chromosome Mapping , Chromosomes, Human, 1-3 , Cell Line , Chromosome Aberrations , HumansABSTRACT
Chromosome material of human and Chinese hamster origin can be stained differentially in human-Chinese hamster translocation chromosomes by use of the Giemsa-11 staining technique.
