Factors Affecting Platelet Concentration in Platelet Concentrates from Canine Blood Donors.

BACKGROUND: Physiologic factors in dogs that might contribute to enhanced platelet yield in platelet concentrates (PCs) are largely unknown. OBJECTIVE: To determine whether individual differences in weight, age, preprocessing blood chemistry, and CBC variables predict the final platelet concentrations in PCs. Our hypotheses were (1) increased lipemic indices would be positively associated with increased platelet concentrations in PCs and (2) increased preprocessing platelet concentrations would be associated with higher platelet concentrations in the PCs. ANIMALS: All blood donation records of dogs from February 2, 2009 through April 1, 2015 at the University of California-Davis Veterinary Blood Bank were examined with 104 cases included in this study. METHODS: In this retrospective study, data were collected from medical records of canine blood donors. Records were reviewed for internal consistency and accuracy and subjects were included in the study if donor screening and donation occurred on the same day and a viable PC was obtained. Univariate and multivariable regressions were used to test the impact that each variable had on the final platelet concentration in PCs. RESULTS: Final platelet concentration in PCs was positively associated with the predonation CBC platelet values (P < .001), lipemic index (P = .01), and phosphorous levels (P = .001). Collectively these 3 variables explained 29% of the variance in platelet concentrations in PCs. CONCLUSIONS AND CLINICAL IMPORTANCE: Future prospective studies are required to determine if canine blood donations from dogs with lipemia yield PCs with higher platelet concentrations without negatively affecting other blood components.

Patologia vásculo-renal / Renovascular diseases

Como órgano apo-exocrino, el riñón cumple con dos importantes funciones: el control de la homeostasis y de la presión arterial. Cualquier patología que altere el flujo sanguíneo del riñón tendrá dos efectos inmediatos: la hipertensión arterial (HA) y la insuficiencia renal (IR). Su corrección debe buscar prioritariamente la mejoría de la función renal ya que la respuesta de la HA vendrá por añadidura. El Objetivo de este trabajo es el de actualizar la sofisticada metodología diagnóstica (Angiografía, EcoDoppler, Angio-Tac, Angio-Resonancia y Angioscopia; el uso de contrastes de potenciación), de funcionalidad (Radionefrograma o RMN con Captopril) y terapéutica (Angioplastia transluminal percutánea, Stents simples o cubiertos, Embolizaciones, uso de Balones oclusivos, Fibrinolisis y Tromboaspiración, Cirugía arterial directa y Autotrasplantación renal), en y de las distintas patologías vasculares renales (Lesiones traumáticas vasculares, Estenosis, Aneurismas, Fístulas arteriovenosas, Oclusiones agudas o Trombosis arterial y venosa, Síndrome de Cascanueces). Se apunta y se discute la posibilidad de existir un Síndrome de Fraley venoso inverso a raíz de un caso clínico. Conclusiones. Se establece una controversia de las tecnologías punta más actuales estableciéndose unas líneas guía de actuación para cada situación patológica. (AU)

[Vasculo-renal disease]. / Patologia vásculo-renal.

UNLABELLED: The kidney as an apo-exocrine organ has two important functions: the control of homeostasis and arterial blood pressure. Any pathological disorders witch alters the renal blood flow results in two consequences: renovascular hypertension and renal insufficiency. Renal revascularization looks for with priority the improvement of renal function; the good response and control of hypertension shall come after. The goals of this paper is to actualize the sophisticated diagnostic methods (Angiography, Eco-Doppler, AngioCT, AngioMR, Angioscopy; and the use of contrast potential-mediums), functionality tests (RN and MR with Captopril) and management (Percutaneous transluminal angioplasty, simple or covered Stents, Embolizations, Occlusive balloons, Fibrinolysis and Trombus Aspiration; direct Arterial Surgery and renal Autotransplantation) of different renovascular diseases. (Blunt renal injuries, Stenosis, Aneurysms. A-V fistuls, Acute Occlusions or arterial and venous, Thrombosis. Nutcraker Syndrome). The possibility of the presence of an inverse venous Fraley's Syndrome is presented and discussed. CONCLUSIONS: A controversy of the last technical methods are established and discussed and a Guideline is recommended for each renal vascular disease.

Passive transfer of seronegative myasthenia gravis to mice.

Muscle weakness in myasthenia gravis is due to autoantibody-induced loss of functional acetylcholine receptors (AChR). About 15% of myasthenia gravis patients, however, do not have detectable anti-AChR antibodies. To investigate the effect of their plasma immunoglobulins on neuromuscular transmission, mice were injected with plasma (and in some cases purified immunoglobulin G (IgG)) from 7 "seronegative" myasthenia gravis (SMG) patients, and neuromuscular transmission parameters were examined. When injected for 15 days, all patients' plasma caused reductions in miniature endplate potential amplitudes, while endplate potential quantal content was significantly reduced by plasma from 4 of the 7 patients. There were no changes in ACh-induced depolarization or single channel properties, and 125I-alpha-bungarotoxin binding studies showed no effect on AChR number, except in 1 case. Purified IgG injected for 3 days had similar effects to plasma injected for 15 days. Our findings confirm that SMG is autoantibody mediated and that there are pathogenic IgG antibodies. SMG appears to be a heterogeneous disorder and the target(s) for the antibodies may be diverse.

[A retropharyngeal synovial sarcoma]. / Sarcoma sinovial retrofaríngeo.

The synovial sarcomas constitute the 7-10% of the soft tissue malign tumors, with a preferred location in the limbs. In the head and neck a 5% of the totality are placed. We present one case of retropharyngeal synovial sarcoma and the accomplished treatment, together with a revision of the bibliography.

The identification of leaf thionin as one of the main jasmonate-induced proteins of barley (Hordeum vulgare).

Jasmonic acid (JA) and its methyl ester (JA-Me) are able to introduce the accumulation of several specific polypeptides in cut leaf segments of barley. Two of the most prominent JA-induced proteins of M(r) 15,000 and 23,000 have been characterized by isolating and sequencing complete cDNA sequences. While the sequence of the M(r) 23,000 polypeptide shows no similarity to published sequences, the sequence of the M(r) 15,000 polypeptide corresponds to the higher-molecular-weight precursor of a leaf thionin previously characterized. Transcripts for the M(r) 23,000 and M(r) 15,000 polypeptides accumulate in leaf segments shortly after the beginning of JA treatment. JA and JA-Me induce the appearance of the two proteins not only in leaf segments but also in intact barley seedlings. However, in seedlings the accumulation of JA-induced proteins occurs much more slowly and requires high concentrations of volatile JA-Me. Thus, in barley it seems unlikely that volatile JA-Me is involved in the interaction between different members of this species, as has been proposed recently for tomato seedlings.

A myasthenia gravis plasma immunoglobulin reduces miniature endplate potentials at human endplates in vitro.

A particular myasthenia gravis (MG) plasma Ig has previously been shown to block a single alpha-bungarotoxin (alpha-BuTx) binding site on embryonic rat muscle acetylcholine receptor (AChR). We have investigated its effect on embryonic/denervated and adult human AChR both in extracts and in situ. Plasma Ig blocked 125I-alpha-BuTx binding by greater than 85% to the AChR extracted from denervated muscle, but only by 55% to AChR extracted from normal human muscle. Incubation of intact human muscle fibers with the plasma Ig reduced 125I-alpha-BuTx binding to the endplate AChRs by 63%, and substantially decreased the amplitude of miniature endplate potentials. We conclude that anti-alpha-BuTx site antibodies, when present, can be important in the pathophysiology of the disease.