ABSTRACT
Using the cell model of regenerative cardiomyogenesis (formation of contracting cardiomyocyte colonies from resident stem cells), we found that the addition of cardiomyocyte-derived apoptotic bodies to the culture of neonatal myocardial cells stimulated proliferation and differentiation of cardiomyocyte precursors and the frequency of their contraction was 1.5-fold higher than in the control. Systemic administration of cardiomyocyte-derived apoptotic bodies to Wistar rats with chronic postinfarction heart failure during the early period of myocardial remodeling considerably improved the contractile function of the heart.
Subject(s)
Extracellular Vesicles/metabolism , Heart Failure/therapy , Myocardial Contraction/physiology , Myocytes, Cardiac/cytology , Animals , Atrial Remodeling/physiology , Cell Culture Techniques , Cell Differentiation/physiology , Cell Proliferation/physiology , Cells, Cultured , Myocardium/cytology , Rats , Rats, Wistar , Ventricular Remodeling/physiologyABSTRACT
Experiments performed on mice C57Bl/6 with the use of GFP technology showed that activation of apoptosis is a potent physiological stimulus for targeted migration of mesenchymal stem cell from the blood to tissues. The effect of apoptosis-induced targeted migration of stem cells can be used in cell therapy of damaged organs and tissues in various pathological states.
Subject(s)
Apoptosis/physiology , Cell Movement/physiology , Mesenchymal Stem Cells/physiology , Animals , Cell- and Tissue-Based Therapy/methods , Green Fluorescent Proteins/metabolism , Mice , Mice, Inbred C57BL , Microscopy, FluorescenceABSTRACT
In modeling acute diphtheric toxemia in guinea pigs, the authors propose to perform hemosorption using selective sorbents under continuous introduction of antitoxic immune sera into extracorporeal contour in front of sorption column. Combination of hemosorption with serotherapy in the same time interval may be denoted as serosorption. As specific sorbents, affine preparations imasorb A-700 and Imasorb G-700 were used. They selectively eliminate from the blood flow CIC produced by diphtheric toxin (anatoxin) and antitoxic rabbit and horse antibodies. Changes in the titers of the diphtheric toxin (anatoxin) and CIC in blood evidence for efficiency of selective sorbents were confirmed by immunohistochemical analysis of the preparations' granules after hemoperfusion.
Subject(s)
Diphtheria/therapy , Hemoperfusion/methods , Sepharose/analogs & derivatives , Toxemia/therapy , Animals , Bacterial Proteins , Diphtheria/immunology , Diphtheria Toxoid/blood , Female , Guinea Pigs , Immune Sera , Ligands , Male , Recombinant Proteins , Staphylococcus aureus , Streptococcus , Toxemia/immunologyABSTRACT
The antitoxic antidiphtheric immunosorbent DATs was tested in experiments on mice and guinea-pigs using the model of acute diphtheric toxemia. In extracorporeal connecting with the circulation system of the animals the sorbent effectively bound and eliminated from the circulation molecules of diphtheric toxin and antitoxin, reduced significantly their levels in the blood after hemoperfusion. The effectiveness of the immunosorbent DATs was confirmed when it saved the ginea-pigs after injection of lethal toxin doses.
