ABSTRACT
In experiments of the 5, 12 and 25-day old rat pups and adult rats in has been shown that after administration of yellow fluorescent protein (YFP) into stomach, its partial absorption in the non-degraded state in the small intestine takes place, with subsequent transport to kidney with blood flow and accumulation in cells of the proximal nephron segment. With age of rats, intensity of the intestinal YFP absorption decrease; the YFP accumulation in the kidney is more active in rats of the younger age groups than in adult animals. No accumulation of YFP in liver was revealed. The obtained data indicate an intensive absorption of YFP in the non-hydrolyzed form in the rat pup small intestine in early postnatal ontogenesis and an important role of kidney in protein metabolism and in proteolysis of exogenous proteins.
Subject(s)
Bacterial Proteins/metabolism , Intestine, Small/metabolism , Kidney/metabolism , Luminescent Proteins/metabolism , Absorption , Animals , Bacterial Proteins/pharmacokinetics , Enterocytes/metabolism , Intestine, Small/ultrastructure , Kidney/growth & development , Kidney/ultrastructure , Luminescent Proteins/pharmacokinetics , Nephrons/growth & development , Nephrons/metabolism , Rats , Rats, WistarABSTRACT
Intestine absorption of intact green fluorescent protein (GFP) and its following accumulation in the renal proximal tubule cells after its intragastric administration have been established by confocal microscopy in the rat and frog. Reabsorbed GFP was revealed in the endosomes and lysosomes of the proximal tubule cells by the methods of GFP photooxidation and immunofluorescent microscopy. The GFP intestine absorption rate and GFP accumulation in the kidney were significantly higher in the frog than in the rat. No specific fluorescence was revealed in the liver and colon cells after the GFP intragastric administration. The data obtained indicate the ability of the small intestine in the frog and rat to absorb intact proteins and an important role of the kidney in exogenous protein metabolism.
Subject(s)
Anura/metabolism , Green Fluorescent Proteins/metabolism , Intestine, Small/metabolism , Kidney Function Tests , Absorption , Animals , Female , Fluorescent Antibody Technique , Green Fluorescent Proteins/administration & dosage , Green Fluorescent Proteins/ultrastructure , Intestine, Small/cytology , Kidney Tubules, Proximal/cytology , Kidney Tubules, Proximal/ultrastructure , Male , Rats , Rats, Wistar , Time FactorsABSTRACT
Yellow fluorescent protein (3.6 ng) was administered through a catheter into the Wistar rat intestine lumen. By the method of confocal microscopy it has been established that as soon as in 3 min this protein appears in the epithelial cells of ileum, is absorbed into the blood and accumulated in cells of the nephron proximal segment, not being revealed in the liver, though. The protein accumulation in kidneys continues for several hours. The yellow fluorescent protein is homogeneously distributed in enterocytes, while in epithelial cells of the proximal tubule this protein is localized in vesicles. The data obtained indicate absorption of non-degraded yellow fluorescent protein in the intestine and role of kidney in metabolism not only of endogenous, but also of exogenous proteins.
