Histopathological Study of Barley Cultivars Resistant and Susceptible to Rhynchosporium secalis.

ABSTRACT Differences in the penetration process by Rhynchosporium secalis were compared in resistant and susceptible barley cultivars at the seedling stage. Percent penetration and percent host cell wall alteration (HCWA) differed significantly among cultivars and isolates as revealed by light microscopy. Based on these two variables, the cultivars were statistically separated into two groups that corresponded to their disease reactions. The resistant cultivars, Johnston and CDC Guardian, showed 81.2 to 99.4% HCWA and 0.1 to 20.1% penetration at encounter sites, whereas the susceptible cultivars, Harrington, Argyle, and Manley, had 30.1 to 78.3% HCWA and 31.8 to 81.8% penetration. In the current study, cv. Leduc, which is susceptible at the seedling stage and resistant at the adult stage, showed the same percent HCWA and penetration as did susceptible cultivars. A significant negative correlation (P < 0.01) was found between percent penetration and percent HCWA for cultivars inoculated with two isolates of the pathogen. Isolate 1 was less virulent than isolate 2 with respect to percent penetration and induced significantly fewer HCWA. Scanning electron microscopy showed various shapes of fungal appressoria but no apparent difference in host reaction between resistant and susceptible cultivars. Transmission electron microscopy revealed interactions between the host and pathogen at various stages of penetration. The resistant cv. Johnston responded by producing appositions, as evidenced by a layer of compact osmiophilic material deposited on the inner side of the cell wall. Infection pegs produced by conidia were unable to penetrate the cuticle where an apposition had formed inside. When penetration occurred in the susceptible cv. Argyle, cytoplasmic aggregates and separation of the plasmalemma were visible from the host cell wall, but the layer of compact osmiophilic material was not always present. Data based on light microscopic observations suggested that HCWA may be one of the mechanisms responsible for resistance that is characterized as penetration prevention rather than as a slow rate of mycelial growth after successful penetration. HCWA occurred in response to attempted cuticle penetration, suggesting that HCWA may produce chemical barriers that help to prevent penetration.

Staining paraffin embedded sections of scald of barley before paraffin removal.

Staining of paraffin embedded sections with periodic acid-Schiff reagent and fast green before paraffin removal resulted in differentiation of barley seed and leaf tissue from fungal structures of Rhynchosporium secalis. Crystal violet, toluidine blue O and antiline blue also successfully stained fungal structures of R. secalis in barley leaf tissues. Staining of embedded sections before paraffin removal allows simple processing of a series of sections, saves time and reduces solvent consumption.

Speech recognition by a deaf-blind multichannel cochlear implant patient.

A case study of the first deaf-blind patient implanted with a Nucleus 22 channel cochlear prosthesis is presented. The patient's preimplant evaluation data, using a powerful hearing aid and vibro-tactile device, are compared with results obtained 8 months postimplant. Preimplant testing indicated no consistent response to sound. Postimplant testing showed ability to comprehend some open set speech with hearing alone through the cochlear implant including ability to utilize the telephone without a code system. The postimplant data suggest potential benefit for other postlingually deaf-blind individuals from multichannel cochlear stimulation.

Intra-aural reflexes elicited by a cochlear prosthesis in monkeys.

Objective audiological tests are needed for pre- and postsurgical evaluation of cochlear prosthesis patients who are unable to give reliable subjective responses. In this study we demonstrated that contralateral intra-aural reflexes were elicited by a cochlear prosthesis in the monkey. Reflex variables measured include threshold, latency and amplitude. These findings indicate that the electrically elicited intra-aural reflex response may be useful to evaluate the peripheral auditory system in subjects with sensory deafness.

Intensity discrimination with cochlear implants.

Intensity difference limens were measured for various frequencies and intensities of sinusoidal and pulsatile electrical stimulation in monkeys with electrodes implanted in the scala tympani, scala vestibuli, modiolus, or middle ear. Difference limens decreased, as a function of initial stimulus intensity, from values of 1.5-3 dB near threshold to as low as 0.5 dB near the upper limit of the dynamic range. If sensation level was held constant, difference limens decreased as a function of frequency up to about 500 Hz, and then remained constant. They were similar across a variety of electrode placements and separations if differences in threshold and dynamic range were taken into account. However, difference limens measured in severely damaged ears were slightly smaller than those in moderately damaged ears. The near miss to Weber's law, characteristic of acoustic difference limens, was not seen with electrical stimuli. Differences limens for electrical stimuli were roughly one-half those for acoustic stimuli; thus, part of the deficit in dynamic range for electrical stimulation compared with acoustic stimulation is countered by the smaller intensity differences limens for electrical stimuli.

Evaluation of tensor tympani muscle dominance in the biphasic acoustic reflex.

The Acoustic reflex frequently causes a biphasic change in impedance at onset. Understanding the cause of the biphasic response is important for establishing a physiological basis for the clinical measurement of reflex latency. The decrease in impedance at onset may be due to uncoupling of impedance contributed by the cochlea. Subsequent increases in impedance predominantly reflect stapedius muscle activity. The clinical implications of this physiologic model are discussed.

Detection, biological effects, and transmission of a virus of the aphid Rhopalosiphum padi.

A virus infecting an Illinois colony of the aphid Rhopalosiphum padi (L.) was transmitted transovarially, and significantly decreased longevity of infected aphids. The virus was detected by serological assay in R. padi colonies from North Dakota, and in two other aphid species maintained at Illinois, R. rufiabdominalis (Sasaki) and Schizaphis graminum (Rondani).

Purification and characterization of a virus from the aphid Rhopalosiphum padi.

A 27-nm icosahedral virus was purified from the oat bird cherry aphid, Rhopalosiphum padi (L.). The virus had an s(20,w) of 162 +/- 2 S, and bouyant densities of 1.37 in CsCl and 1.35 in Cs2SO4. It contained one ssRNA of 31 +/- 2 S and three major proteins. The relationship of the R. padi virus to other small RNA invertebrate viruses is unclear.