ABSTRACT
This article describes the experience of application of multipotent mesenchymal stromal cells in the complex therapy of severe recurrent cholangitis in 2 children with biliary atresia after Kasai surgery. In both children, hepatic cellular insufficiency and portal hypertension developed against the background of long-term inflammatory process poorly controlled by standard therapy, which was the indication for liver transplantation. During the course of mesenchymal stromal cells therapy, the relief of the inflammatory process and functional recovery of the liver were achieved. At the time of preparing the article, the follow-up of two children since the start of multipotent mesenchymal stromal cell therapy was 3 years 9 months and 2 years 6 months. No recurrence of cholangitis was observed in the patients during the follow-up period, the liver function was preserved. There are no indications for liver transplantation at this moment. Thus, despite the fact that the mechanisms of therapeutic action of multipotent mesenchymal stromal cells in biliary atresia require further investigation, we obtained promising results suggesting the possibility of using mesenchymal stromal cells in the treatment of postoperative complications in children with biliary atresia.
Subject(s)
Biliary Atresia , Mesenchymal Stem Cells , Child , Humans , Biliary Atresia/surgeryABSTRACT
We explored the possibility of using postmortem MRI for the diagnostics of lung hypoplasia associated with innate diaphragmatic hernia in neonates. The main experimental group consisted of 17 newborns with innate diaphragmatic hernia including 10 non-operated newborns and 7 newborns died after surgery for innate diaphragmatic hernia. It was demonstrated that postmortem MRI allows objective quantitative assessment of the absolute and relative dimensions of the lungs in the thoracic cavity and thereby reveals their hypoplasia, which contributes to the determination of tanatogenesis. Surgery for congenital diaphragmatic hernia leads to an increase in the mass and volume of the lungs, but does not always eliminate their hypoplasia.
Subject(s)
Abnormalities, Multiple/diagnosis , Lung Diseases/diagnosis , Lung/abnormalities , Magnetic Resonance Imaging/methods , Abnormalities, Multiple/pathology , Autopsy/methods , Female , Hernias, Diaphragmatic, Congenital/complications , Hernias, Diaphragmatic, Congenital/diagnosis , Hernias, Diaphragmatic, Congenital/pathology , Humans , Infant , Infant Death , Infant, Newborn , Lung/pathology , Lung Diseases/complications , Lung Diseases/pathology , Lung Volume Measurements/methods , Male , Predictive Value of Tests , StillbirthABSTRACT
Invasiveness of examination and therapy methods is a serious problem for intensive care and nursing of premature infants. Exhaled breath condensate (EBC) is the most attractive biofluid for non-invasive methods development in neonatology for monitoring the status of intubated infants. The aim of the study was to propose an approach for EBC sampling and analysis from mechanically ventilated neonates. EBC collection system with good reproducibility of sampling was demonstrated. Discovery-based proteomic and metabolomic studies were performed using nano-HPLC coupled to high resolution MS. Label-free semi-quantitative data were compared for intubated neonates with congenital pneumonia (12 infants) and left-sided congenital diaphragmatic hernia (12 infants) in order to define disease-specific features. Totally 119 proteins and 164 metabolites were found. A number of proteins and metabolites that can act as potential biomarkers of respiratory diseases were proposed and require further validation.
Subject(s)
Breath Tests/instrumentation , Chromatography, High Pressure Liquid/instrumentation , Hernias, Diaphragmatic, Congenital/diagnosis , Mass Spectrometry/instrumentation , Pneumonia/diagnosis , Biomarkers/analysis , Equipment Design , Female , Hernias, Diaphragmatic, Congenital/metabolism , Humans , Infant, Newborn , Male , Metabolome , Metabolomics/instrumentation , Pneumonia/metabolism , Proteins/analysis , Proteomics/instrumentation , Reproducibility of ResultsABSTRACT
This study was designed to collect and perform a proteomic analysis of expired air condensate in newborns receiving respiratory support at the Department of Resuscitation and Intensive Care. The proteomic composition of expired air condensate was evaluated in newborns at various stages of development and with different abnormalities.
Subject(s)
Proteome/metabolism , Breath Tests , Exhalation , Humans , Infant, Newborn , Proteomics , Respiration, ArtificialABSTRACT
The article deals with an experience of veno-arterial extracorporeal membrane oxygenation use in preterm infants with congenital diaphragmatic hernia during postoperative period in the perinatal center.
Subject(s)
Critical Care/methods , Extracorporeal Membrane Oxygenation/methods , Hernias, Diaphragmatic, Congenital , Infant, Premature, Diseases/therapy , Perinatal Care/methods , Respiratory Insufficiency/therapy , Fatal Outcome , Hernia, Diaphragmatic/complications , Hernia, Diaphragmatic/surgery , Hernia, Diaphragmatic/therapy , Humans , Infant, Newborn , Infant, Premature , Infant, Premature, Diseases/surgery , Male , Respiratory Insufficiency/etiologyABSTRACT
The article highlights the most urgent issues of anaesthesia and intensive care in obstetrics presented in the reports of the leading specialists at the 2nd All-Russian Educational Congress "Anaesthesia And Intensive Care In Obstetrics And Neonatology".
Subject(s)
Anesthesia, Obstetrical/methods , Anesthesiology/methods , Intensive Care, Neonatal/methods , Neonatology/methods , Female , Humans , Infant, Newborn , RussiaABSTRACT
When in the experiment on rats the jointly immobilized on a carbonic cloth proteases and lytic enzyme complex were used, the reduced period of the suppurative wound cleaning and healing was noted as compared to that in use of the immobilized on a carbonic cloth proteases and jointly immobilized on a gause proteases and lytic enzyme complex.
