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1.
Vet Rec ; 180(18): 447, 2017 May 06.
Article in English | MEDLINE | ID: mdl-28386029

ABSTRACT

Samples from bovine viral diarrhoea virus (BVDV)-positive cattle were gathered by Scottish diagnostic laboratories and used to produce a Biobank of samples with associated location and identification data in support of the Scottish BVDV eradication scheme. The samples were subject to direct amplification and sequencing of the 5'-untranslated region (5'-UTR) to define the viral types and subtypes present. From 2693 samples collected prior to 2016, approximately 2300 sequences were obtained, representing 8 BVDV type 1 subtypes. No BVDV type 2 samples were detected. The samples came from all regions of the UK but 66 per cent were from Scotland. Analysis of the sequences showed great diversity in the 5'-UTR, with 1206 different sequences. Many samples carried virus with identical 5'-UTR sequences; often from single locations, but there were also examples of the same sequence being obtained from samples at several different locations. This work provides a resource that can be used to analyse the movement of BVDV strains both within Scotland and between Scotland and other nations, particularly in the latter stages of the Scottish eradication programme, and so inform the advice available to both livestock keepers and policymakers.


Subject(s)
Bovine Virus Diarrhea-Mucosal Disease/prevention & control , Bovine Virus Diarrhea-Mucosal Disease/virology , Diarrhea Virus 1, Bovine Viral/genetics , Disease Eradication , 5' Untranslated Regions/genetics , Animals , Biological Specimen Banks , Bovine Virus Diarrhea-Mucosal Disease/epidemiology , Cattle , Databases, Nucleic Acid , Diarrhea Virus 1, Bovine Viral/classification , Diarrhea Virus 1, Bovine Viral/isolation & purification , Scotland/epidemiology
2.
Vet Rec ; 154(14): 423-6, 2004 Apr 03.
Article in English | MEDLINE | ID: mdl-15119893

ABSTRACT

After being vaccinated against rabies some cats and dogs fail to show an antibody titre adequate to meet the requirements of the UK Pet Travel Scheme. To investigate this problem, the data derived from 16,073 serum samples submitted to the Veterinary Laboratories Agency for serological testing between 1999 and 2002, 1002 samples submitted to BioBest during March and April 2001, and 1264 samples associated with one make of vaccine submitted to BioBest between June 2001 and January 2003, were analysed. The probability of antibody titre failing to reach at least 0.5 iu/ml was analysed by logistic regression as a function of the choice of vaccine, the interval between vaccination and sampling, the sex and age of the animal, and its country of origin. In dogs, all these factors, except sex, had highly significant (P < 0.001) effects on the test failure rate, and in cats all the factors had a significant effect (P < 0.05).


Subject(s)
Antibodies, Viral/blood , Rabies Vaccines/immunology , Rabies virus/immunology , Rabies/veterinary , Age Factors , Animals , Cat Diseases/prevention & control , Cats , Dog Diseases/prevention & control , Dogs , Female , Logistic Models , Male , Neutralization Tests/veterinary , Rabies/immunology , Rabies/prevention & control , Risk Factors , Sex Factors , Time Factors
3.
Cancer Lett ; 152(1): 9-13, 2000 Apr 28.
Article in English | MEDLINE | ID: mdl-10754200

ABSTRACT

The mdm2 oncogene is amplified and overexpressed in a variety of human tumours and the oncogenic potential of MDM2 is partly due to its ability to inactivate tumour suppressor p53 function. In the present communication we describe the cloning, sequence analysis and expression of the complete wildtype canine and equine mdm2 cDNAs. The encoded full-length canine and equine cDNAs show strong sequence homology with MDM2 proteins from other species and both cDNAs generate recombinant proteins of approximately 90 kDa. These data will allow for the role of this oncogene to be established in companion animal oncology.


Subject(s)
Nuclear Proteins , Proto-Oncogene Proteins/genetics , Amino Acid Sequence , Animals , Base Sequence , Cloning, Molecular , DNA, Complementary , Dogs , Horses , Mice , Molecular Sequence Data , Proto-Oncogene Mas , Proto-Oncogene Proteins/metabolism , Proto-Oncogene Proteins c-mdm2 , Recombinant Proteins/biosynthesis , Sequence Analysis, DNA , Sequence Homology, Amino Acid
4.
Oncogene ; 18(35): 5005-9, 1999 Sep 02.
Article in English | MEDLINE | ID: mdl-10490836

ABSTRACT

The cDNA of porcine p53 was cloned and sequenced by a reverse transcriptase polymerase chain reaction (RT - PCR) approach with primers based on regions of homology between all known p53 sequences. The p53 cDNA was found to be 87% conserved to human p53 and 86% conserved to bovine p53 at the nucleotide level. The porcine p53 sequence was inserted into an expression vector and recombinant protein expressed in vitro. An approximately 50 kDa protein was detected by Western blotting using both polyclonal and monoclonal anti-p53 antibodies. The sequence data of porcine p53 and the ability to detect expressed protein with various anti-p53 antibodies will allow the p53 status of the pig population, and the role of p53 in porcine tumours, to be assessed. An understanding of tumour development in the pig may be important if pig cells, tissues or organs are to be used in the treatment of humans.


Subject(s)
Antibodies/immunology , Genes, p53 , Swine/genetics , Tumor Suppressor Protein p53/analysis , Tumor Suppressor Protein p53/immunology , Amino Acid Sequence , Animals , Antibodies, Monoclonal/immunology , Base Sequence , Blotting, Western , Cloning, Molecular , Conserved Sequence/genetics , Conserved Sequence/immunology , Cross Reactions , Epitopes/genetics , Epitopes/immunology , Humans , Molecular Sequence Data , Molecular Weight , Recombinant Proteins/analysis , Recombinant Proteins/chemistry , Recombinant Proteins/immunology , Sequence Alignment , Tumor Suppressor Protein p53/chemistry , Tumor Suppressor Protein p53/genetics
5.
Vet Microbiol ; 53(3-4): 227-37, 1996 Dec.
Article in English | MEDLINE | ID: mdl-9008334

ABSTRACT

Canine herpesvirus 1 (CHV-1), a member of the alphaherpesvirus sub-family, is known to cause fatal infections in litters of puppies and may also be involved in infertility, abortion, and stillbirths in adult dogs. The purpose of this study was to determine the presence of CHV-1 DNA using the polymerase chain reaction (PCR) in twelve key sites that have been associated with latency for the other herpesviruses. A 605 base pair portion of the viral glycoprotein B (gB) gene was amplified using degenerate primers, cloned, and sequenced. Conventional 20 mer primers were designed using this sequence information to amplify a 120 bp fragment of gB situated between the original degenerate primers. The specificity of amplification was confirmed by Southern Blot hybridisation using an internal oligonucleotide probe. DNA was extracted from tissue samples taken from twelve dogs at post mortem and from twenty-four blood samples. Nine out of twelve dogs showed evidence of infection with CHV-1; the tissues most commonly affected were lumbo-sacral ganglia (5/12 dogs), tonsil (5/12), parotid salivary gland (4/9), and liver (4/9). No positive results were detected within the twenty-four blood samples. These results indicate that exposure to CHV-1 may be much more common than previously suggested.


Subject(s)
Dog Diseases , Herpesviridae Infections/veterinary , Herpesvirus 1, Canid/isolation & purification , Polymerase Chain Reaction/veterinary , Animals , Blotting, Southern , DNA Primers , DNA, Viral/analysis , Dogs , Female , Ganglia, Spinal/virology , Herpesviridae Infections/diagnosis , Liver/virology , Oligonucleotide Probes , Organ Specificity , Palatine Tonsil/virology , Parotid Gland/virology , Placenta/virology , Polymerase Chain Reaction/methods , Pregnancy , Sheep , Viral Envelope Proteins/genetics
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