ABSTRACT
Changes in the peptide composition of hemolymph of Galleria mellonella larvae induced by their immunization have been studied, and some new peptides have been found. The composition of fractions exhibiting antibacterial activity was investigated. Known antibacterial peptides have been found in the hemolymph of control larvae and those immunized with bacteria.
Subject(s)
Anti-Bacterial Agents/chemistry , Hemolymph/metabolism , Lepidoptera/metabolism , Peptides/chemistry , Animals , Anti-Bacterial Agents/pharmacology , Bacillus cereus/immunology , Bacterial Vaccines/immunology , Chromatography, High Pressure Liquid , Escherichia coli/immunology , Larva/metabolism , Lepidoptera/growth & development , Peptides/pharmacology , Spectrometry, Mass, Matrix-Assisted Laser Desorption-IonizationABSTRACT
A new bioregulator operating in ultralow doses corresponding to 10(-17) mg/ml has been isolated from tissue of pigmented epithelium of bovine eyes. It has been established that the functional basis of this bioregulator is a complex of a low molecular weight regulatory peptide (4372 Da) and a modulator consisting of a mixture of proteins with molecular weights of 14.980-66.283 kDa. It has been shown that the regulatory peptide is responsible for membranotropic activity of the bioregulator, and the modulator proteins are responsible for biological action in ultralow doses. The data demonstrate an interrelation between nanocondition of the bioregulator and its ability to show activity in ultralow doses.
