ABSTRACT
Dynamic mixtures of Rh-dye complexes can be used to determine the history of chemical events such as the addition of ATP and ADP by UV-vis spectroscopy.
ABSTRACT
A multicomponent indicator displacement assay (MIDA) based on an organometallic receptor and three dyes can be used for the identification and quantification of nucleotides in aqueous solution at neutral pH.
Subject(s)
Nucleotides/analysis , Water/chemistry , Solutions , Spectrophotometry, UltravioletABSTRACT
A dynamic combinatorial library (DCL) of dye complexes of Cu2+ and Ni2+ was used to sense the sequence isomeric tripeptides His-Gly-Gly, Gly-His-Gly, and Gly-Gly-His. The DCL sensor was obtained by mixing buffered aqueous solution of three commercially available dyes together with CuCl2 and NiCl2. The addition of the peptide analytes resulted in characteristic changes in the UV-vis spectrum of the mixture, which allowed the identification of the peptide. Due to the modular nature of the sensor, it was possible to optimize the sensor by variation of the amounts and ratio of its constituent building blocks. The composition of the best sensor was found to vary substantially, depending on the sensing problem to be addressed. Whereas a Cu2+/Ni2+ ratio of 1:3 gave the best differentiation for Gly-His-Gly and Gly-Gly-His, a sensor containing exclusively Cu2+ was found to provide the best discrimination between His-Gly-Gly and Gly-Gly-His.
Subject(s)
Coloring Agents/chemistry , Combinatorial Chemistry Techniques , Metals/chemistry , Oligopeptides/chemistry , Peptide Library , Amino Acid Sequence , Copper/chemistry , Isomerism , Molecular Sequence Data , Nickel/chemistry , Spectrophotometry, UltravioletABSTRACT
Indicator-displacement assays based on the organometallic complex [{Cp*RhCl2}2] (Cp*=pentamethylcyclopentadienyl) and the dye gallocyanine were used to sense amino sugars and aminoglycosides in buffered aqueous solution by conducting UV-visible spectroscopy. The data of three assays at pH 7.0, 8.0, and 9.0 were sufficient to distinguish between the amino sugars galactosamine, glucosamine, mannosamine and the aminoglycosides kanamycin A, kanamycin B, amikacin, apramycin, paromomycin, and streptomycin. Furthermore, the assays were used to characterize mixtures of aminoglycosides and obtain quantitative information about the respective analytes.
Subject(s)
Amino Sugars/analysis , Aminoglycosides/analysis , Chemistry Techniques, Analytical/methods , Organometallic Compounds/chemistry , Carbohydrate Sequence , Molecular Sequence Data , Oxazines/chemistry , Rhodium/chemistry , Sensitivity and Specificity , Spectrophotometry, UltravioletABSTRACT
Twenty natural amino acids can be distinguished with high fidelity using an array of indicator displacement assays in combination with a multivariate analysis. The array is easily constructed by mixing of commercially available building blocks at different pH.
