The Role of Representational Gestures and Speech Synchronicity in Auditory Input by L2 and L1 Speakers.

Speech and gesture are two integrated and temporally coordinated systems. Manual gestures can help second language (L2) speakers with vocabulary learning and word retrieval. However, it is still under-investigated whether the synchronisation of speech and gesture has a role in helping listeners compensate for the difficulties in processing L2 aural information. In this paper, we tested, in two behavioural experiments, how L2 speakers process speech and gesture asynchronies in comparison to native speakers (L1). L2 speakers responded significantly faster when gestures and the semantic relevant speech were synchronous than asynchronous. They responded significantly slower than L1 speakers regardless of speech/gesture synchronisation. On the other hand, L1 speakers did not show a significant difference between asynchronous and synchronous integration of gestures and speech. We conclude that gesture-speech asynchrony affects L2 speakers more than L1 speakers.

Capturing Cross-linguistic Differences in Macro-rhythm: The Case of Italian and English.

We tested the hypothesis that languages can be classified by their degree of tonal rhythm (Jun, 2014). The tonal rhythms of English and Italian were quantified using the following parameters: (a) regularity of tonal alternations in time, measured as durational variability in peak-to-peak and valley-to-valley intervals; (b) magnitude of F0 excursions, measured as the range of frequencies covered by the speaker between consecutive F0 maxima and minima; (c) number of tonal target points per intonational unit; and (d) similarity of F0 rising and falling contours within intonational units. The results show that, as predicted by Jun's prosodic typology (2014), Italian has a stronger tonal rhythm than English, expressed by higher regularity in the distribution of F0 minima turning points, larger F0 excursions, and more frequent tonal targets, indicating alternating phonological H and L tones. This cross-language difference can be explained by the relative load of F0 and durational ratios on the perception and production of speech rhythm and prominence. We suggest that research on the role of speech rhythm in speech processing and language acquisition should not be restricted to syllabic rhythm, but should also examine the role of cross-language differences in tonal rhythm.

Relative Salience of Speech Rhythm and Speech Rate on Perceived Foreign Accent in a Second Language.

We investigated the independent contribution of speech rate and speech rhythm to perceived foreign accent. To address this issue we used a resynthesis technique that allows neutralizing segmental and tonal idiosyncrasies between identical sentences produced by French learners of English at different proficiency levels and maintaining the idiosyncrasies pertaining to prosodic timing patterns. We created stimuli that (1) preserved the idiosyncrasies in speech rhythm while controlling for the differences in speech rate between the utterances; (2) preserved the idiosyncrasies in speech rate while controlling for the differences in speech rhythm between the utterances; and (3) preserved the idiosyncrasies both in speech rate and speech rhythm. All the stimuli were created in intoned (with imposed intonational contour) and flat (with monotonized, constant F0) conditions. The original and the resynthesized sentences were rated by native speakers of English for degree of foreign accent. We found that both speech rate and speech rhythm influence the degree of perceived foreign accent, but the effect of speech rhythm is larger than that of speech rate. We also found that intonation enhances the perception of fine differences in rhythmic patterns but reduces the perceptual salience of fine differences in speech rate.

Chemical and pathological oxidative influences on band 3 protein anion-exchanger.

BACKGROUND/AIMS: The erythrocyte is a cell exposed to a high level of oxygen pressure and to oxidative chemical agents. This stress involves SH-groups oxidation, cell shrinkage by activation of K-Cl co-transport (KCC) and elevation of the band 3 tyrosine phosphorylation level. The aim of our study was to test whether oxidative stress could influence band 3-mediated anion transport in human red blood cells. METHODS: To evaluate this hypothesis, normal and pathological (glucose 6 phosphate dehydrogenase (G6PDH) defficient) erythrocytes were treated with known sulphydryl-blocking or thiol-oxidizing agents, such as N-ethylmaleimide (NEM), azodicarboxylic acid bis[dimethylamide] (diamide), orthovanadate, Mg2+ and tested for sulphate (SO4-) uptake, K+ efflux, G6PDH activity and glutathione (GSH) concentration. RESULTS: In normal red blood cells, the rate constants of SO4- uptake decreased by about 28 % when cells were incubated with NEM, diamide and orthovanadate. In G6PDH-deficient red blood cells, in which oxidative stress occurs naturally, the rate constant of sulphate uptake was decreased by about 40% that of normal red cells. Addition of oxidizing and phosphatase inhibitor agents to pathological erythrocytes further decreased anion transport. In contrast, G6PDH activity was increased under oxidative stress in normal as well as in pathological cells and was lower in the presence of exogenous Mg2+ in parallel to a significant increase in sulphate transport. In both cells, the oxidizing agents increased K+ efflux with depletion of GSH. CONCLUSION: The data are discussed in light of the possible opposite effects exerted by oxidative agents and Mg2+ on KCC and on the protein tyrosine kinase (PTK)-protein tyrosine phosphatase (PTP) equilibrium. The decreased sulphate uptake observed in the experimental and pathological conditions could be due to band 3 SH-groups oxidation or to oxidative stress-induced K-Cl symport-mediated cell shrinkage with concomitant band 3 tyrosine phosphorylation.

Toll-like receptor 4 (TLR4) does not confer a resistance advantage on mice against low-dose aerosol infection with virulent type A Francisella tularensis.

Francisella tularensis, the causative agent of tularemia, is a gram-negative facultative intracellular bacterium. Toll-like receptor (TLR) 4 is considered to be critical for inducing host innate immunity against many gram-negative bacteria including many respiratory pathogens. To determine the role of TLR4 in host defense against airborne F. tularensis infection, TLR4-defective C3H/HeJ (TLR4(d)) or wild-type C3H/HeOuJ (WT) mice were challenged by low-dose aerosol with type A F. tularensis, and the course of the infection and host responses were compared at day 2 and 4 post-inoculation (dpi). At dpi 2, bacterial burdens in the lungs were similar between TLR4(d) and WT mice, but TLR4(d) mice surprisingly harbored approximately 10-fold fewer bacteria in their spleens and livers. However, the bacterial burdens at dpi 4, the mortality and median time to irreversible moribundity were indistinguishable between the two mouse strains. In addition, the inflammatory responses to the infection, as reflected by the cytokine levels and leukocyte influx in the bronchoalveolar lavage fluid and histopathological analysis, were similar between both mouse strains. Additionally, as with C3H mice, we found no difference in either the median time to death or the survival rate between TLR4-deleted C57BL/10ScNJ mice and WT C57BL/10 mice. Combined, these data suggest that TLR4 does not contribute to resistance of mice to airborne type A F. tularensis infection.

A disc-based quantitative carrier test method to assess the virucidal activity of chemical germicides.

Suspension tests for virucidal activity of chemical germicides are easier to perform, but they normally do not present the test product with a strong enough challenge. In contrast, carrier tests, where the test virus is dried on an animate or inanimate surface, offer the test formulation a higher level of challenge because it first has to penetrate successfully the inoculum to gain access to and inactivate the target organism on the carrier. Since pathogens in nature are normally found adsorbed to surfaces and/or embedded in organic or cellular debris, the results of carrier tests are more relevant to predicting the activity of chemical germicides under field situations. The method described below uses discs (1 cm in diameter) of brushed stainless steel discs as carriers. Ten micro l of the test virus in a soil load is placed on each disc and the inoculum dried under ambient conditions. The dried inoculum is then exposed to 50 micro l of the test formulation or a control solution for a defined contact time at the specified temperature. EBSS (0.95 ml) is added to each carrier holder to dilute/neutralize the germicide, the inoculum eluted and the eluates titrated in cell cultures to determine the degree of loss in virus viability. At least five test and three control carriers are used in each test. Controls are also included to test for toxicity of the test formulation to the host cells and any interference sub-cytotoxic levels of the formulation may have on the ability of the virus to infect the cells. The method has been used with several types of human and animal pathogenic viruses to test the activity of all major classes of chemical germicides against them.