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1.
Front Cell Neurosci ; 16: 837576, 2022.
Article in English | MEDLINE | ID: mdl-35444517

ABSTRACT

Emerging evidence suggests that DNA repair deficiency and genome instability may be the impending signs of many neurological diseases. Genome-wide association (GWAS) studies have established a strong correlation between genes that play a role in DNA damage repair and many neurodegenerative diseases, including Huntington's disease (HD), and several other trinucleotides repeat expansion-related hereditary ataxias. Recently, many reports have documented a significant role played by the DNA repair processes in aging and in modifying many neurodegenerative diseases, early during their progression. Studies from our lab and others have now begun to understand the mechanisms that cause defective DNA repair in HD and surprisingly, many proteins that have a strong link to known neurodegenerative diseases seem to be important players in these cellular pathways. Mutations in huntingtin (HTT) gene that lead to polyglutamine repeat expansion at the N-terminal of HTT protein has been shown to disrupt transcription-coupled DNA repair process, a specialized DNA repair process associated with transcription. Due to the recent progress made in understanding the mechanisms of DNA repair in relation to HD, in this review, we will mainly focus on the mechanisms by which the wild-type huntingtin (HTT) protein helps in DNA repair during transcription, and the how polyglutamine expansions in HTT impedes this process in HD. Further studies that identify new players in DNA repair will help in our understanding of this process in neurons. Furthermore, it should help us understand how various DNA repair mechanism(s) coordinate to maintain the normal physiology of neurons, and provide insights for the development of novel drugs at prodromal stages of these neurodegenerative diseases.

2.
Biol Open ; 10(1)2021 01 27.
Article in English | MEDLINE | ID: mdl-33504470

ABSTRACT

The success of antiretroviral therapy (ART) has improved the survival of HIV-infected patients significantly. However, significant numbers of patients on ART whose HIV disease is well controlled show peripheral sensory neuropathy (PSN), suggesting that ART may cause PSN. Although the nucleoside reverse transcriptase inhibitors (NRTIs), one of the vital components of ART, are thought to contribute to PSN, the mechanisms underlying the PSN induced by NRTIs are unclear. In this study, we developed a Drosophila model of NRTI-induced PSN that recapitulates the salient features observed in patients undergoing ART: PSN and nociceptive hypersensitivity. Furthermore, our data demonstrate that pathways known to suppress PSN induced by chemotherapeutic drugs are ineffective in suppressing the PSN or nociception induced by NRTIs. Instead, we found that increased dynamics of a peripheral sensory neuron may possibly underlie NRTI-induced PSN and nociception. Our model provides a solid platform in which to investigate further mechanisms of ART-induced PSN and nociceptive hypersensitivity.This article has an associated First Person interview with the first author of the paper.


Subject(s)
Antiretroviral Therapy, Highly Active/adverse effects , Nociceptive Pain/etiology , Peripheral Nervous System Diseases/etiology , Animals , Anti-HIV Agents/adverse effects , Anti-Retroviral Agents/adverse effects , Disease Models, Animal , Drosophila , HIV Infections/complications , HIV Infections/drug therapy , Humans , Nociceptive Pain/diagnosis , Peripheral Nervous System Diseases/diagnosis , Sensory Receptor Cells
3.
Sci Rep ; 8(1): 16099, 2018 10 31.
Article in English | MEDLINE | ID: mdl-30382129

ABSTRACT

Functional synaptic networks are compromised in many neurodevelopmental and neurodegenerative diseases. While the mechanisms of axonal transport and localization of synaptic vesicles and mitochondria are relatively well studied, little is known about the mechanisms that regulate the localization of proteins that localize to active zones. Recent finding suggests that mechanisms involved in transporting proteins destined to active zones are distinct from those that transport synaptic vesicles or mitochondria. Here we report that localization of BRP-an essential active zone scaffolding protein in Drosophila, depends on the precise balance of neuronal Par-1 kinase. Disruption of Par-1 levels leads to excess accumulation of BRP in axons at the expense of BRP at active zones. Temporal analyses demonstrate that accumulation of BRP within axons precedes the loss of synaptic function and its depletion from the active zones. Mechanistically, we find that Par-1 co-localizes with BRP and is present in the same molecular complex, raising the possibility of a novel mechanism for selective localization of BRP-like active zone scaffolding proteins. Taken together, these data suggest an intriguing possibility that mislocalization of active zone proteins like BRP might be one of the earliest signs of synapse perturbation and perhaps, synaptic networks that precede many neurological disorders.


Subject(s)
Drosophila Proteins/metabolism , Drosophila melanogaster/metabolism , Glycogen Synthase Kinase 3/metabolism , Neuromuscular Junction/metabolism , Synapses/metabolism , Animals , Axons/metabolism , Axons/ultrastructure , Larva/metabolism , Larva/ultrastructure , Microtubule-Associated Proteins/metabolism , Presynaptic Terminals/metabolism , Protein Transport , Synapses/ultrastructure
4.
PLoS Genet ; 13(5): e1006822, 2017 May.
Article in English | MEDLINE | ID: mdl-28562608

ABSTRACT

[This corrects the article DOI: 10.1371/journal.pgen.1006621.].

5.
PLoS Genet ; 13(2): e1006621, 2017 02.
Article in English | MEDLINE | ID: mdl-28222093

ABSTRACT

Disruption of synapses underlies a plethora of neurodevelopmental and neurodegenerative disease. Presynaptic specialization called the active zone plays a critical role in the communication with postsynaptic neuron. While the role of many proteins at the active zones in synaptic communication is relatively well studied, very little is known about how these proteins are transported to the synapses. For example, are there distinct mechanisms for the transport of active zone components or are they all transported in the same transport vesicle? Is active zone protein transport regulated? In this report we show that overexpression of Par-1/MARK kinase, a protein whose misregulation has been implicated in Autism spectrum disorders (ASDs) and neurodegenerative disorders, lead to a specific block in the transport of an active zone protein component- Bruchpilot at Drosophila neuromuscular junctions. Consistent with a block in axonal transport, we find a decrease in number of active zones and reduced neurotransmission in flies overexpressing Par-1 kinase. Interestingly, we find that Par-1 acts independently of Tau-one of the most well studied substrates of Par-1, revealing a presynaptic function for Par-1 that is independent of Tau. Thus, our study strongly suggests that there are distinct mechanisms that transport components of active zones and that they are tightly regulated.


Subject(s)
Autism Spectrum Disorder/genetics , Drosophila Proteins/genetics , Glycogen Synthase Kinase 3/genetics , Neuromuscular Junction/genetics , tau Proteins/genetics , Animals , Autism Spectrum Disorder/pathology , Axons/metabolism , Drosophila , Drosophila Proteins/metabolism , Glycogen Synthase Kinase 3/metabolism , Humans , Neurons/metabolism , Neurons/pathology , Presynaptic Terminals/metabolism , Presynaptic Terminals/pathology , Protein Transport/genetics , Synapses/genetics , Synapses/pathology , Synaptic Transmission/genetics
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