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Vopr Virusol ; 38(6): 249-53, 1993.
Article in Russian | MEDLINE | ID: mdl-7905690

ABSTRACT

Polymerase chain reaction (PCR) was developed for the detection of simian T-lymphotropic virus type 1 (STLV-1) infection of P. hamadryas and direct sequencing using oligo-nucleotide primer pairs specific for the tax and env regions of the related human T-lymphotropic virus type 1 (HTLV-1). Excellent specificity was shown in the detection of STLV-1 provirus in infected baboons by PCR using HTLV-1-derived primers. The nucleotide sequences of env 467bp and tax 159bp of the proviral genome (env position 5700-6137, tax position 7373-7498 HTLV-1, according to Seiki et al., 1983) derived from STLV-1-infected P. hamadryas were analysed using PCR and direct sequencing techniques. Two STLV-1 isolates from different sources (Sukhumi main-SuTLV-1 and forest stocks-STLV-1F) were compared. Two variants of STLV-1 among P. hamadryas with different level of homology to HTLV-1 were wound (83.8% and 95.2%, respectively). A possible role of nucleotide changes in env and tax sequenced fragments and oncogenicity of STLV-1 variants is discussed.


Subject(s)
Genetic Variation/genetics , Papio/microbiology , Simian T-lymphotropic virus 1/genetics , Animals , Base Sequence , DNA, Viral/genetics , DNA, Viral/isolation & purification , Deltaretrovirus Infections/microbiology , Deltaretrovirus Infections/veterinary , Georgia (Republic) , Lymph Nodes/microbiology , Lymphocytes/microbiology , Molecular Sequence Data , Polymerase Chain Reaction , Proviruses/genetics , Sequence Analysis, DNA
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