ABSTRACT
The phagocyte activity of rat alveolar macrophages and their primary culture under the interaction with liposomes containing unsaturated fatty acid complex Bien has been studied in vitro. Quantitative dependence of the phagocyte number and phagocyte factor on the liposomal Bien concentration were estimated. Comparative analysis of the effects of multilayered and unilayered liposomes with Bien upon cell suspension has been carried out.
Subject(s)
Fatty Acids, Unsaturated/pharmacology , Macrophages, Alveolar/metabolism , Phagocytosis/drug effects , Unilamellar Liposomes/pharmacology , Animals , Macrophages, Alveolar/cytology , Rats , Rats, WistarABSTRACT
Phagocytic activity of alveolar macrophages isolated from rat bronchoalveolar lavage fluid was studied during their reaction with multilamellar liposomes containing biene (a complex of unsaturated fatty acids). Liposomes with incorporated biene significantly stimulated phagocytic activity of freshly isolated macrophages and primary culture of these cells (judging from the increase in phagocytic index and phagocytic number). A quantitative relationship between the phagocytic number and phagocytic index, on the one hand, and the concentration of liposomal biene, on the other, was detected. The effects of liposomes containing incorporated biene, liposomes of similar composition without biene, and pure biene substance on cell suspension were compared.
Subject(s)
Fatty Acids, Unsaturated/pharmacology , Liposomes/pharmacology , Macrophages, Alveolar/drug effects , Phagocytosis/drug effects , Animals , Cells, Cultured , Liposomes/chemistry , Macrophages, Alveolar/cytology , RatsABSTRACT
The conditions of fatty acid complex "bien" incorporation into multilayered liposomes prepared by mechanical dispersion techniques (vortexing) have been investigated. Drug incorporation depends on the lipid composition used to prepare the vesicles, overall lipid and "bien" concentrations and the presence of an antioxidant. Vortexing and incubation regimes for the homogeneous liposomal suspension were determined. The effects of contents of DL-alpha-tocopherol and cholesterol on lipid peroxidation were evaluated.
