Complete mitogenome and intra-family comparative mitogenomics showed distinct position of Pama Croaker Otolithoides pama.

The Pama Croaker, Otolithoides pama, is an economically important fish species in Bangladesh. Intra-family similarities in morphology and typical barcode sequences of cox1 create ambiguities in its identification. Therefore, morphology and the complete mitochondrial genome of O. pama, and comparative mitogenomics within the family Sciaenidae have been studied. Extracted genomic DNA was subjected to Illumina-based short read sequencing for De-Novo mitogenome assembly. The complete mitogenome of O. pama (Accession: OQ784575.1) was 16,513 bp, with strong AC biasness and strand asymmetry. Relative synonymous codon usage (RSCU) among 13 protein-coding genes (PCGs) of O. pama was also analyzed. The studied mitogenomes including O. pama exhibited consistent sizes and gene orders, except for the genus Johnius which possessed notably longer mitogenomes with unique gene rearrangements. Different genetic distance metrics across 30 species of Sciaenidae family demonstrated 12S rRNA and the control region (CR) as the most conserved and variable regions, respectively, while most of the PCGs undergone a purifying selection. Different phylogenetic trees were congruent with one another, where O. pama was distinctly placed. This study would contribute to distinguishing closely related fish species of Sciaenidae family and can be instrumental in conserving the genetic diversity of O. pama.

Community-driven PPE production using additive manufacturing during the COVID-19 pandemic: Survey and lessons learned.

This study presents a detailed analysis of the production efforts for personal protective equipment in makerspaces and informal production spaces (i.e., community-driven efforts) in response to the COVID-19 pandemic in the United States. The focus of this study is on additive manufacturing (also known as 3D printing), which was the dominant manufacturing method employed in these production efforts. Production details from a variety of informal production efforts were systematically analyzed to quantify the scale and efficiency of different efforts. Data for this analysis was primarily drawn from detailed survey data from 74 individuals who participated in these different production efforts, as well as from a systematic review of 145 publicly available news stories. This rich dataset enables a comprehensive summary of the community-driven production efforts, with detailed and quantitative comparisons of different efforts. In this study, factors that influenced production efficiency and success were investigated, including choice of PPE designs, production logistics, and additive manufacturing processes employed by makerspaces and universities. From this investigation, several themes emerged including challenges associated with matching production rates to demand, production methods with vastly different production rates, inefficient production due to slow build times and high scrap rates, and difficulty obtaining necessary feedstocks. Despite these challenges, nearly every maker involved in these production efforts categorized their response as successful. Lessons learned and themes derived from this systematic study of these results are compiled and presented to help inform better practices for future community-driven use of additive manufacturing, especially in response to emergencies.