Administration of antigen by intralymphatic route in the rat. II. Results in tumor active immunotherapy (delta). Preliminary report.

Tumor growth and survival time were followed in rats transplanted with Morris hepatoma 5123 and previously injected with a crude homogenate of the same tumor by the intradermic or intralymphatic route. Female rats treated by the intralymphatic route showed a longer survival time and impaired tumor growth. Male rats did not show any difference.

Administration of antigen by intralymphatic route in the rat I. Humoral response to soluble antigens. (delta). Preliminary report.

Male rats were immunized against bovine albumin injected by either intradermic or intralymphatic routes. A significantly higher number of animals treated by the latter route did respond, probably because antigens introduced by this route directly reach lymph nodes, by passing blood and tissue lytic enzymes.

Technique of lymphography in the rat.

Most the lymphatic system in the rat was directly observed in our experiment by radiological means. The lymphatics of the limbs were firstly revealed by subcutaneous injection of Patent Blue V. Lipiodol was used as radio-opaque compound injected by a micro-technique fully described by the authors.