ABSTRACT
The stability of ifosfamide in Ringer lactate buffer solution either alone or mixed with mesna at 37 degrees C for a 7-day period was analyzed by HPLC. This study was performed to investigate the feasibility of continuous infusion of ifosfamide by a multiday pump in order to reduce the toxicity and to increase the production of active alkylating metabolites of the parent drug. The total decay of ifosfamide activity did not exceed 3.2% at day 7. We conclude that ifosfamide can be safely delivered in a 7-day infusion with no significant loss of activity.
Subject(s)
Antineoplastic Agents, Alkylating/chemistry , Ifosfamide/chemistry , Antineoplastic Agents, Alkylating/administration & dosage , Chromatography, High Pressure Liquid , Drug Stability , Ifosfamide/administration & dosage , Infusion Pumps , Mesna/chemistry , Solutions , Time FactorsSubject(s)
Papio/microbiology , Simian Immunodeficiency Virus/isolation & purification , Simian T-lymphotropic virus 1/isolation & purification , Animals , Antibodies, Viral/blood , Female , Gabon/epidemiology , Male , Monkey Diseases/epidemiology , Monkey Diseases/immunology , Monkey Diseases/transmission , Retroviridae Infections/epidemiology , Retroviridae Infections/transmission , Retroviridae Infections/veterinary , Simian Acquired Immunodeficiency Syndrome/epidemiology , Simian Acquired Immunodeficiency Syndrome/immunology , Simian Acquired Immunodeficiency Syndrome/transmissionABSTRACT
Two cases of wild-born chimpanzees which were positive for HIV-1 antibodies were observed in Gabon. These animals were never experimentally exposed to HIV-1 and had no history of inoculation with human blood products. A retrovirus was isolated from one of these chimpanzees. Several of the viral proteins from this virus, designated SIVcpz-GAB-1 (simian immunodeficiency virus from chimpanzee), differed in molecular weight from the known corresponding HIV/SIV proteins. The major gag protein of SIVcpz migrated on SDS-PAGE with a relative molecular mass of 25.5 and the outer membrane proteins were 110, 155 and 185 kD, respectively. SIVcpz did not induce severe cytopathic effects in human and chimpanzee lymphocytes. Antigenically, SIVcpz seems to be closer to HIV-1 than to HIV-2 and the other SIVs. Nucleic acid hybridization experiments appear to indicate that the virus is different from HIV-1 and HIV-2.
Subject(s)
Antibodies, Viral/immunology , HIV Antibodies/immunology , Pan troglodytes/microbiology , Simian Immunodeficiency Virus/isolation & purification , Animals , Antibody Specificity , Cells, Cultured , Cross Reactions , Cytopathogenic Effect, Viral , Gabon , HIV/immunology , Humans , Lymphocytes/microbiology , Nucleic Acid Hybridization , Pan troglodytes/immunology , RNA, Viral/analysis , Retroviridae Proteins/analysis , Simian Immunodeficiency Virus/classification , Simian Immunodeficiency Virus/immunology , Species Specificity , Virus CultivationABSTRACT
After some short notices on the physiopathology of thrombosis, the AA. consider clinic, symptomatology and surgical therapy for prevention of pulmonary embolism. They outline that only a precocious operation and an exact diagnosis can help the patient who had had an embolism; in the end they report two cases, describing the operation and its indications.
