ABSTRACT
Four cases of peritonitis caused by the filamentous fungus Paecilomyces variotii in patients on continuous ambulatory peritoneal dialysis are reported. Removal of the Tenckhoff catheter and antifungal chemotherapy led to resolution of symptoms in all cases. Possible contaminating events are discussed, and reported infections with P. variotii are reviewed.
Subject(s)
Hemodialysis Solutions , Mycoses/microbiology , Paecilomyces/isolation & purification , Peritoneal Dialysis, Continuous Ambulatory/adverse effects , Peritonitis/microbiology , Adult , Catheters, Indwelling/adverse effects , Child , Female , Humans , Male , Middle Aged , Paecilomyces/growth & developmentABSTRACT
The activities of trimethoprim and sulfamethoxazole, singly and in combination, were compared with the activities of six other antimicrobial drugs against 50 anaerobic bacteria isolated from clinical specimens. Trimethoprim alone had little activity against the anaerobes tested, but sulfamethoxazole and the sulfamethoxazole/trimethoprim combination were active against most of the organisms. Results with the combination were slightly better than those with sulfamethoxazole alone.
Subject(s)
Bacteria, Anaerobic/drug effects , Sulfamethoxazole/pharmacology , Trimethoprim/pharmacology , Drug Combinations/pharmacology , Microbial Sensitivity Tests , Trimethoprim, Sulfamethoxazole Drug CombinationABSTRACT
An improved, rapid and accurate enzymatic adenylation assay for gentamicin has been developed. It can be completed in one hour with an accuracy of +/- 2.7% (+/- 1 SD) for the range of gentamicin concentrations of 6-12 microgram/ml. Concentrated enzyme was used to prepare a complete adenylation mixture, which was stored frozen in aliquots suitable for daily assays. A special apparatus designed for efficient washing of phosphocellulose disks was used in the assay.
Subject(s)
Gentamicins/blood , Adenosine Triphosphate/metabolism , Gentamicins/metabolism , Humans , Medical Laboratory Science/instrumentation , MethodsABSTRACT
A new device has been developed for efficient washing of phosphocellulose disks used in enzymatic assays of antibiotics. Its construction and operation are described. The device allows all disks to receive identical washing, including agitation, and the sequence of the disks is maintained. A comparison has been made of the results of adenylation assay of gentamicin using the new device and hand-washing of the paper disks.
