[Migration and differentiation of transplanted fetal neurogenic cells in animals with brain ischemia].

The migration, integration and differentiation of fetal neural progenitor cells (NPCs) in the ischemic brain have been studied. In our study the ischemic insult in FVB line mice was produced by occlusion of both carotid arteries during 20 min. A day after occlusion NPCs from GFP-transgenic 12.5 dpc embryos were suboccipitally transplanted to the ischemic brain. The migration and differentiation of GFP-positive NPCs in the recipient tissue were observed in different time points after their transplantation by immunohistochemical approaches using confocal scanning microscopy. It has been shown that GFP-positive NPCs survived, migrated and differentiated to the mature neurons and glial cells in CA1 area of hippocampus of ischemic animals.

[Increase in T-cell level in the peripheral blood of women with fractures in anamnesis during postmenopause period].

44 postmenopausal patients (45-76 years of old) have been observed. Interrelationship between composition and functional activity of peripheral blood cells, TNF-alpha level in the serum and bone density in postmenopausal women has been analyzed. It was established that the increase in neutrophil activity in women with osteoporosis, the high level of immune complexes are accompanied by the high level of IgG and autoantibodies to DNA. These results allow suggesting the presence of chronic inflammatory or immune processes taken place in postmenopausal women with osteoporosis. Increase in CD3+ cells percent was established in women with fracture in anamnesis. Obtained results allow suggesting the possibility that T-cells may play a role in the occurrence of fractures.

[Role of genetic factors in the development of osteoporosis].

Osteoporosis which often accompanies the normal aging and a number of pathological conditions is closely related to bone remodeling disturbances. This process is provided mainly by the genesis of osteoblasts and osteoclasts, their activities and interactions. All the above in turn is dependent on numerous systemic and local regulatory factors such as hormones, nutrients, minerals, vitamins, cytokines, their receptors etc. Recently the crucial role of genetic polymorphism of many receptors in bone remodeling, bone loss and susceptibility to osteoporosis development has been shown. This review is devoted to the genetic background of interrelationships among the hormones, cytokines, vitamins and their receptors and discussion of possible sources of discrepancies in data obtained by many authors who studied populations like genetic, diet, life-styles and environmental influences.

[Age and the vascular reaction to injury]. / Vik ta reaktsiia sudyn na poshkodzhennia.

Our own results and the literature dates analysis showed that spontaneous endothelial cells injury in aging, and the hypertension, hypercholesterinemia, immune disorders, that was induced in experiment aggravating the process of the development of atherosclerosis.

[The characteristics of the development of the immune function in the transplanted spleen of newborn mouse pups in recipients of different ages. 2. The effect of the thymus on the functional development of the transplant]. / Osoblyvosti rozvytku imunnoï funktsiï peresadzhenoï selezinky navonarodzhenykh mysheniat retsypiientam riznoho viku. 2. Vplyv tymusa na funktsional'nyi rozvytok transplantata.

The effect of the neonatal thymus grafting or "Thymostimulin" administration on the cellularity, cell composition, immune, response to SRBC and proliferative activity of T- and B-cells in vitro were determined in neonatal spleen grafted CBA/Ca female mice of different ages. Analysis of the thymus graft effect on the T- and B-cells content in the spleen transplant from the adult and old recipients demonstrated no differences. The neonatal thymus grafting led to the essential increase of the immune response, spleen cellularity and to the diametrically opposed changing from negative to positive of the sign of the correlation coefficient between the T-cells content and the cellularity of the neonatal spleen in the old recipients. The similar effect of the neonatal thymus grafting was revealed in respect of correlative connection between content of the T-cells and PFCs in spleen grafted to the old recipients too. The "Thymostimulin" injection led only to the increase of the spleen transplant cellularity. These results suggests that the young thymic microenvironment is essential for the normal T-cells differentiation and for its normal function in the neonatal spleen transplant.

[The characteristics of the development of the immune function in the transplanted spleen of newborn mouse pups in recipients of different ages. I. The effect of the cells in the stromal microenvironment of the transplant]. / Osoblyvosti rozvytku imunnoï funktsiï peresadzhenoï selezinky novonarodzhenykh mysheniat retsypiientam riznoho viku. I. Vplyv klityn stromal'noho mikrootochennia transplantata.

The age-related changes in the cell composition, immune response to SRBC and proliferative activity of T- and B-cells in vitro were determined in neonatal spleen grafted to CBA/Ca female mice of different ages. The dependence of the immune response upon the recipient's age was observed. This result indicate that "young" transplant stromal cells are incapable of immune response increase in the old recipients. Analysis of the T- and B-cells content in the spleen grafted to the recipients of different ages demonstrated no differences. The decrease of the T-cell proliferative activity and existence of the inverse correlative connection between content of the T-cells and PFCs in spleen grafted to the old recipients were demonstrated. Additional neonatal thymus transplantation is proposed as tools for correction of the age-related changes in the immune function.

[Effect of the changes of epiphysial functions on the central and peripheral immune system in mice]. / Vplyv zmin funktii epifiza na stan tsentralnoi ta peryferychnoi lanok imunnoi systemy u myshei.

Endocrine function of thymus estimated by titer of thymic serum factor is inhibited in pubertal mice CBA/Ca under conditions of pharmacological inhibition of epiphysis function by beta-adrenoblocking agent anapriline. After blinding and epithalamine and melatonine introduction the content of thymic hormone considerably increases. Melatonine is characterized by manifestation of effect in earlier periods of research. In mice with hyperfunction of epiphysis mass, cell character of thymus and spleen increase as well as the content of thymosine-like activity in supernatant of cultures of splenocytes. And on the contrary, in animals with hypofunction of epiphysis the values of studied immunological indices decrease. Immunopotentiating effect of epiphysial factors is observed not only in vivo but also in vitro and decreases in thymectomized mice. The change of the concentration of glucocorticoids in the organism is of significance in the mechanism of epiphysis effect on the immune system.