ABSTRACT
Calostoma insigne puffball mushrooms are only found in forests with rich biodiversity in very few countries including Thailand, and their biofunctions remain largely unexplored. This study used the agar disk diffusion assay, the anti-glucosidase assay, and the 3, 4, 5-dimethylthiazol-2-yl-2-5-diphenyltetrazolium bromide (MTT) assay to evaluate the bioactive potential of these endangered puffball mushrooms. Internal transcribed spacer (ITS) gene analysis identified C. insigne, a puffball mushroom with green, globose, and spiny spores. Fourier-transform infrared spectroscopy (FTIR) analysis confirmed the polysaccharide structure while scanning electron microscopy (SEM) revealed a fiber-like network. The ethanolic gelatinous fruiting body extract exhibited 1,1-diphenyl-2-picrylhydrazyl (DPPH)-scavenging capacity (57.96%), a ferric ion-reducing antioxidant power (FRAP) value of 1.73 mg FeSO4/g, and α-glucosidase inhibition (73.18%). C. insigne cytotoxicity was effective towards HT-29 colon cancer cells using the MTT assay (IC50 of 770.6 µg/mL at 72 h) and also showed antiproliferative capacity (IC50 of 297.1 µg/mL). This puffball mushroom stimulated apoptotic genes and proteins (caspase-3, Bax, and p21) via an intrinsic apoptotic pathway in HT-29 cells. In the laboratory, the medium formula consisting of 20% potato, 2% sucrose, and 0.2% peptone was optimal to increase fungal mycelial biomass (2.74 g DW/100 mL), with propagation at pH 5.0 and 30 °C. Puffball mushrooms are consumed as local foods and also confer several potential health benefits, making them worthy of conservation for sustainable utilization.
ABSTRACT
Twenty-five wild edible mushrooms from Northeastern Thailand were analyzed for their antioxidant activities, proteins, sugars, ß-glucan, and phenolic profiles. Results showed that T. clypeatus and V. volvacea exhibited the greatest scavenging activity (83.07 and 86.60%) and reductive power (9.79 and 8.42 g Fe(II)/kg, respectively). T. clypeatus recorded the highest content of (+)-catechin and naringenin (13.40 and 0.74 g/kg dw), with V. volvacea the highest amount of quercetin and quercetin-3-O-rutinoside (1.82 and 1.16 g/kg dw, respectively). Both T. clypeatus and V. volvacea also exhibited the greatest amounts of ß-glucan (125.23 and 344.43 g/kg dw) and protein (343.30 and 452.20 g/kg dw, respectively) among the mushroom species evaluated. Results suggested that both T. clypeatus and V. volvacea were a good source of healthy compounds, namely ß-glucan and flavonoids, and could be used to mitigate diseases involving free radicals.
ABSTRACT
Phenolic compounds extracted from fourteen Mao-Luang (Antidesma bunius) cultivars grown in Northeastern Thailand were quantified using HPLC analysis and the antioxidant capacity of the extracts was determined by ABTS(+), DPPH radical, and FRAP assays. This study showed that there were differences (p<0.05) in phenolic compounds and antioxidant activities among Mao-Luang cultivars. Gallic acid, (-)-epicatechin, (+)-catechin, and cyanidin-3-O-glucoside were the major polyphenolic components in all Mao-Luang cultivars. The highest contents were found in 'Kumlai' with values of 281.30, 949.73, 127.60 and 54.67 mg/100g DW, respectively. 'Kumlai' also exhibited the highest antioxidant activity with the values of 103.04 mmol VCEAC/gDW (DPPH assay), 35.35 mmol Fe(II)/gDW (FRAP assay), and 46.37 mmol TE/gDW (ABTS(+) assay). Correlation analyses revealed that gallic acid, ferulic acid and some anthocyanins, mainly cyanidin-3-O-glucoside, were responsible for the antioxidant capacity of Mao-Luang fruits.
