[Pyogenic infections with different locations caused by Streptococcus anginosus alone or in association with anaerobic bacteria]. / Infectii piogene cu localizari diferite cauzate de Streptoccus anginosus, singur sau în asociatie cu bacterii anaerobe.

Isolation and identification of S. anginosus from pathologic products are possible to be realised in informed clinical laboratory, allowing so a better knowledge of these infections' incidences and on adequate treatment. The authors' report 11 cases of pyogenic infection caused by S. anginosus. Five out of these 11 cases evaluated as mixed infections, S. anginosus being associated with anaerobic bacteria. 10 children hospitalised in surgery Department of Children Hospital had infections with different localisations; necrotizing fasciitis, preknee cap abscess, generalised peritonitis, abscess postappendectomy, pleurisy and acute mediastinitis, knee arthritis, acute osteomyelitis of mandible and an infection of the fracture's focus in upper 1/3 of the thigh bone. For all these patients the favoring factor was represented by a traumatic or surgical lesion of the skin or diverse mucosa; oral, oesophageal, intestinal, allowing the access of the normal flora of these covers to normally sterile sites. The eleventh case was an adult with a lung abscess and pleurisy, as a complication of an aspiration pneumonia. The treatment of S. anginosus infections consisted especially in penicillin or ampicillin, associated with metronidazol when anaerobic bacteria were present.

[The frequency of Streptococcus anginosus in the pharyngeal exudate from children and its differentiation from other beta-hemolytic streptococci]. / Frecventa Streptococcus anginosus în exsudatul faringian la copii si diferentierea de alti streptococi beta-hemolitici.

UNLABELLED: S. anginosus is a commensal of the oro-pharyngeal mucous membrane without any signification for the local pathology. Ignoring the existence of beta-haemolytic colonies of this species, the risk to report the presence of some beta-haemolytic streptococci that actually belong to the normal flora exists. The antigenic identifications of beta-haemolytic streptococci maintain the confusion either, S. anginosus being able to react with specific sera anti group G, C or A. In our study, two identification criteria out of those available demonstrated a high value: the small or very small colonies' size, especially in secondary cultures and the production of acetoin (Voges-Proskauer test). S. anginosus was isolated with a quite great frequency in pharyngeal exudate from children: 41 strains out of 90 strains of beta-haemolytic non-group A streptococci were S. anginosus. Antigenically they belonged, in numerical order to groups C, G, F or they were ungroupable. IN CONCLUSION: The microbiologist has to identify, but not to report the presence of S. anginosus in pharyngeal exudate, it being a normal component of the oro-pharyngeal flora. Doing so, a better evaluation of the clinical signification of the other beta haemolytic streptococci's non group A will be possible.

The role of catecholamines in the distribution of steroid hormones in the striated and cardiac muscle.

Loss of muscular strength is a well-known component of the clinical picture many endocrine diseases and is often their predominant aspect. It has recently been proved that the cytoplasmatic receptors for steroid hormones (SH) are artefacts of the fixation techniques and that they are attached to the internal surface of the cell membrane. Our experiment was made on male and female. Wistar rats which were tested for the level of radioactively labelled steroid hormones (3H and HD3H hydrocortisone; 3H testosterone, TS3H; 3H estrone, FS3H) in various types of skeletal muscles (from rapid to slow) and cardiac muscle before and after adrenalin administration. Before adrenalin administration one noticed: (a) a significantly higher level of HD3H (p < 0.05) in the female skeletal muscle; (b) in the femoral biceps and psoas, a significantly higher distribution of TS3H in males (p < 0.01), whereas in the diaphragm and the heart, there were no significant differences between sexes; (c) there are significant differences between sexes as regards the ES3H level in any of the studied muscles. After adrenalin administration there was a significant decrease in the level of labelled SH uptake in all experimental schemes. It is possible that adrenalin block the HS diffusion through the plasma membrane or even their binding to specific receptors.

The role of catecholamines in muscular growth.

This paper reports on an experiment concerning the involvement of catecholamines in the biosynthesis of nucleic acids and proteins at muscular levels, using various types of skeletal muscles (psoas, diaphragm, soleus) and heart muscles from male Wistar rats treated with adrenalin. Assessment of the biosynthetic processes was made by the uptake level of 3H thymidine, 3H uridine, and 3H triptophan in the muscles. The experiment showed the followings: a) nucleic acids and protein synthesis stayed unchanged in the psoas; b) adrenalin stimulated the uptake of the radioactive precursors in the diaphragm and soleus, and c) the biosynthetic processes were inhibited in the myocardium by adrenalin administration. Though adrenalin is known to stimulate proteic synthesis in the skeletal muscle, our study showed that this process occurs only in certain types of muscles (with slow contraction). Inhibition of proteic synthesis in the myocardium in the presence of adrenalin might be one of the cellular mechanisms involved in cardiac failure.

Steroid hormones control on nucleic acid biosynthesis in skeletal muscle.

The medical, agricultural and scientific interest of the muscular tissue is well established in the literature. Its formation and function are essential for survival. It is also known that steroid hormones are involved in the growth, development and maturation of skeletal muscles (sexual hormones) as well as in the process of body adjustment to the stress factors in the environment (glucocorticoid hormones). Starting from these considerations, our experiment has made an attempt to clarify part of the mechanisms involved in the action of steroid hormones at muscle level. On this purpose, 3H thymidine and 3H uridine incorporation was followed up in various types of skeletal muscles (femoral biceps, diaphragmatic, psoas) from rats treated with steroid hormones. Though known as anabolic hormones, sexual hormones did not induce significant and persistent changes in the nucleic acid synthesis (NAS), except for testosterone which enhanced RNA synthesis only in the level femoral muscle after 21 days of administration. Progesterone and the glucocorticoid hormones are known as hormones of proteic catabolism but it seems that this effect is more marked in muscles whose structure predominantly consists of white fibres (rapid muscles), as confirmed by our experiment.