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1.
Biull Eksp Biol Med ; 116(10): 407-9, 1993 Oct.
Article in Russian | MEDLINE | ID: mdl-8117967

ABSTRACT

Quick model of experimental atherosclerosis--3 weeks of cholesterol feeding and 3 weeks of standard diet for aorta atheromatosis development--was used in rabbits. Then one group of animals was fed 1 month by plant oil of specific composition, with added omega-3 (linolenic) fatty acid, and the other group received the same dose of fish oil. The most intensive decrease of plasma cholesterol and erythrocyte cholesterol/phospholipid ratio was in rabbits, that received the oil with linolenic acid. Triglyceride level decreased gradually in all animal groups in the same degree, and HOL cholesterol did not change. After the end of experiment the aorta damage degree in group, that received plant oil with linolenic acid, was only 10.8%, as compared with 36.2% in control group (without treatment) and 39.3% in rabbits, that received fish oil. The absence of fish oil effect supports the earlier data about rabbits specific sensitivity to this product resulting in possible hepatotoxic effect. The data indicates on the usefulness of rabbit model in fish oil effects investigations, and also on the positive, antiatherogenic effect of plant oil with added linolenic acid.


Subject(s)
Arteriosclerosis/drug therapy , Fatty Acids, Omega-3/therapeutic use , Plant Oils/therapeutic use , Animals , Arteriosclerosis/blood , Diet, Atherogenic , Disease Models, Animal , Drug Evaluation, Preclinical , Drug Therapy, Combination , Fish Oils/therapeutic use , Linoleic Acid , Linoleic Acids/therapeutic use , Male , Rabbits , Time Factors
2.
Biull Eksp Biol Med ; 109(5): 492-4, 1990 May.
Article in Russian | MEDLINE | ID: mdl-2378967

ABSTRACT

The changes of hepatic mitochondrial cells were studied in white male rats during internal application of boron-containing waters, having 250 mg/l concentration of boron, but different ionic-salt basis. Mitochondrial hepatocytes and hepatic boron content relationships were established. It was shown that during boron accumulation in liver (15 applications) decreasing of mitochondrial size occurred, independently, of the type of boron-containing waters. During stable boron level and particularly during its excretion from organism (24 applications, 6 days aftereffect), increase of size of mitochondria and their ellipticity, change of configuration, that depend on the type of applied waters was shown.


Subject(s)
Boron/adverse effects , Mitochondria, Liver/ultrastructure , Water Supply , Animals , Boron/administration & dosage , Boron/analysis , Liver/analysis , Liver/drug effects , Male , Mitochondria, Liver/drug effects , Models, Biological , Rats , Spectrophotometry
3.
Vestn Dermatol Venerol ; (1): 20-2, 1990.
Article in Russian | MEDLINE | ID: mdl-2327166

ABSTRACT

Lymphocytic nuclei interphase chromatin was examined with the use of automated morphometry in psoriasis patients treated by different methods. Fifty optic and geometric parameters of lymphocytes were under study; the nuclear size and relative contrast, relative contrast of nongranular component, and chromatin pattern determined visually were found the most informative characteristics. The results evidence significant differences between the reference group of normal subjects and psoriasis patients. After treatment a tendency to normalization of the shifted parameters of interphase chromatin can be traced; PUVA therapy intensifies this process, and the parameters reach the reference values.


Subject(s)
Chromatin/ultrastructure , Interphase , Psoriasis/blood , Adult , Humans , Lymphocytes/ultrastructure , Middle Aged , PUVA Therapy , Psoriasis/drug therapy , Vitamin B Complex/therapeutic use
4.
Biull Eksp Biol Med ; 107(5): 637-9, 1989 May.
Article in Russian | MEDLINE | ID: mdl-2736306

ABSTRACT

The morphometric analysis of the interphase chromatine structure of the hepatic cells nuclei was carried out on the automated TV installation for the quantitative analysis of images "IBAS-2" (by the OPTON firm, the FRG) according to 50 optical and geometric parameters during various periods (1.2 and 4 weeks) after the vagotomy operation. It is determined that upper-molecular organisation of chromatine undergoes the biggest changes one week after operation, and changes of granular component are more informative than changes of the nongranular component (with the difference 15-20%). It was also revealed that chromatine components differ in tinctorial properties, which are evidently dependent on physicochemical characteristics of the chromatine under various functional conditions of the cell. As a result of the correlation analysis the group of morphometric indices of chromatine structure was revealed, which are highly correlated with level of transcription activity of chromatine during various terms after denervation. The correlation quotient of these parameters is 0.85-0.97. The summing up: vagus denervation of the liver causes changes in the morphofunctional organisation of the chromatine.


Subject(s)
Cell Nucleus/ultrastructure , Chromatin/ultrastructure , Liver/ultrastructure , Vagotomy , Animals , Liver/innervation , Male , Microscopy, Electron/instrumentation , Rats , Software , Television/instrumentation , Time Factors
5.
Article in English | MEDLINE | ID: mdl-3449133

ABSTRACT

Following are the results of using the elaborated algorhythms of the structural analysis for the study of the form elements of the blood. A possibility was demonstrated to carry out a differential diagnosis of the familial hyperlipoproteidemia of the second type according to the quantitative structural parametres of the chromatin of the inter-phase nuclei of the lymphocytes of the peripheral blood. A method was developed of the automated morphometry of erythrocytes making it possible to assess the optical profile of erythrocytes according to 16 parametres. It was also registered a more vivid change of the curve of the membrane of the erythrocytes as compared with the Price-Jones spread on the same erythrocytes. With a help of a system of proposed parametres, which are 4-6-fold more informative and sensitive, than the traditional ones, it is possible to obtain the qualitatively new characteristics of the forms of erythrocytes, and not only their sizes.


Subject(s)
Cytodiagnosis/methods , Hyperlipoproteinemias/diagnosis , Chromatin/ultrastructure , Erythrocytes/ultrastructure , Humans , Hyperlipoproteinemias/blood , Hyperlipoproteinemias/genetics , Interphase , Lymphocytes/ultrastructure
6.
Biull Eksp Biol Med ; 99(6): 726-7, 1985 Jun.
Article in Russian | MEDLINE | ID: mdl-2410060

ABSTRACT

Geometric and optical parameters of chromatin of hepatocyte nuclei have been examined before (UV, lambda = 265 nm) and after gallocyanine staining. Quantitative parameters of the chromatin structure in the same nuclei measured in situ by a scanning microscope-photometer (step size 0.125 micron) before and after staining were equal. Tinctorial properties of chromatin granules (condensed part of the nuclear material) and its diffuse part were different. It is suggested that the difference between granules and the nongranular part of chromatin is not only of optical but also of chemical nature.


Subject(s)
Cell Nucleus/ultrastructure , Chromatin/ultrastructure , Interphase , Animals , Flow Cytometry/methods , Liver/ultrastructure , Mice , Oxazines , Sciuridae , Staining and Labeling/methods , Ultraviolet Rays
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