ABSTRACT
We report a 4-year-old girl presenting with sudden severe bilateral visual loss. Ophthalmological examination revealed optic disc pallor. Further neurological examination was normal. Brain magnetic resonance imaging (MRI) suggested chiasmal optic neuritis, and further etiological investigations were negative. We review the literature on the incidence and underlying etiology of chiasmal optic neuritis in childhood.
Subject(s)
Optic Chiasm/pathology , Optic Neuritis/diagnosis , Child, Preschool , Female , Humans , Magnetic Resonance Imaging , Optic Neuritis/complications , Vision Disorders/etiologyABSTRACT
OBJECTIVE: We have recently shown that de novo mutations in the TUBA1A gene are responsible for a wide spectrum of neuronal migration disorders. To better define the range of these abnormalities, we searched for additional mutations in a cohort of 100 patients with lissencephaly spectrum for whom no mutation was identified in DCX, LIS1 and ARX genes and compared these data to five previously described patients with TUBA1A mutations. RESULTS: We detected de novo TUBA1A mutations in six patients and highlight the existence of a prominent form of TUBA1A related lissencephaly. In four patients, the mutations identified, c.1190T>C (p.L397P), c.1265G>A (p.R422H), c.1264C>T (p.R422C), c.1306G>T (p.G436R), have not been reported before and in two others, the mutation corresponds to a recurrent missense mutation, c.790C>T (p.R264C), likely to be a hot spot of mutation. All together, it emerges that the TUBA1A related lissencephaly spectrum ranges from perisylvian pachygyria, in the less severe form, to posteriorly predominant pachygyria in the most severe, associated with dysgenesis of the anterior limb of the internal capsule and mild to severe cerebellar hypoplasia. When compared with a large series of lissencephaly of other origins (ILS17, ILSX or unknown origin), these features appear to be specific to TUBA1A related lissencephaly. In addition, TUBA1A mutated patients share a common clinical phenotype that consists of congenital microcephaly, mental retardation and diplegia/tetraplegia. CONCLUSIONS: Our data highlight the presence of consistent and specific abnormalities that should allow the differentiation of TUBA1A related lissencephalies from those related to LIS1, DCX and ARX genes.
Subject(s)
Lissencephaly/genetics , Tubulin/genetics , Child , Child, Preschool , Female , Heterozygote , Humans , Infant , Lissencephaly/pathology , Magnetic Resonance Imaging , Male , Mutation, Missense , Phenotype , Tubulin/chemistryABSTRACT
BACKGROUND: Lissencephaly is a neuronal migration disorder leading to absent or reduced gyration and a broadened but poorly organized cortex. The most common form of lissencephaly is isolated, referred as classic or type 1 lissencephaly. Type 1 lissencephaly is mostly associated with a heterozygous deletion of the entire LIS1 gene, whereas intragenic heterozygous LIS1 mutations or hemizygous DCX mutations in males are less common. METHODS: Eighteen unrelated patients with type 1 lissencephaly were clinically and genetically assessed. In addition, patients with subcortical band heterotopia (n = 1) or lissencephaly with cerebellar hypoplasia (n = 2) were included. RESULTS: Fourteen new and seven previously described LIS1 mutations were identified. We observed nine truncating mutations (nonsense, n = 2; frameshift, n = 7), six splice site mutations, five missense mutations, and one in-frame deletion. Somatic mosaicism was assumed in three patients with partial subcortical band heterotopia in the occipital-parietal lobes or mild pachygyria. We report three mutations in exon 11, including a frameshift which extends the LIS1 protein, leading to type 1 lissencephaly and illustrating the functional importance of the WD domains at the C terminus. Furthermore, we present two patients with novel LIS1 mutations in exon 10 associated with lissencephaly with cerebellar hypoplasia type a. CONCLUSION: In contrast to previous reports, our data suggest that neither type nor position of intragenic mutations in the LIS1 gene allows an unambiguous prediction of the phenotypic severity. Furthermore, patients presenting with mild cerebral malformations such as subcortical band heterotopia or cerebellar hypoplasia should be considered for genetic analysis of the LIS1 gene.
Subject(s)
1-Alkyl-2-acetylglycerophosphocholine Esterase/genetics , Cerebral Cortex/abnormalities , Genetic Predisposition to Disease/genetics , Microtubule-Associated Proteins/genetics , Mutation/genetics , Nervous System Malformations/genetics , Adolescent , Adult , Cell Movement/genetics , Cerebellum/abnormalities , Cerebral Cortex/metabolism , Cerebral Cortex/physiopathology , Child , Child, Preschool , Choristoma/genetics , Choristoma/metabolism , DNA Mutational Analysis , Female , Genetic Markers/genetics , Genetic Testing , Genotype , Humans , Infant , Male , Nervous System Malformations/metabolism , Nervous System Malformations/physiopathology , Penetrance , PhenotypeABSTRACT
Hereditary sensory and autonomic neuropathy type II (HSAN-II) is caused by recessive mutations in the HSN2 gene assigned to chromosome 12p13.33. The authors report three unrelated HSAN-II families with homozygous or compound heterozygous mutations resulting in the truncation of the HSN2 protein. Genotype-phenotype correlations indicated that HSN2 mutations are associated with an early childhood onset of a predominantly sensory neuropathy, complicated by acromutilations in both upper and lower limbs.
Subject(s)
Chromosomes, Human, Pair 12 , Hereditary Sensory and Autonomic Neuropathies/genetics , Mutation , Nerve Tissue Proteins/genetics , Adult , Base Sequence , Chromosome Mapping , Exons , Female , Genetic Carrier Screening , Genotype , Humans , Intracellular Signaling Peptides and Proteins , Introns , Male , Middle Aged , Minor Histocompatibility Antigens , Pedigree , Phenotype , Protein Serine-Threonine Kinases , Sequence Deletion , WNK Lysine-Deficient Protein Kinase 1ABSTRACT
We examined the efficacy, optimum dosage and adverse effects of levetiracetam in two prospective trials in children with epilepsy. In the add-on trial, 67 children between 6 months and 16 years were included. In the mono-therapy trial, 10 children between 4 years and 16 years were included. Levetiracetam was titrated up to an optimal dosage for every individual patient, depending on efficacy and tolerability, and reflecting clinical practice. The range of dosages used was between 12 and 62 mg/kg/day, with a median of 33 mg/kg/day. Overall, 20 weeks after the start of levetiracetam, there was a median seizure reduction of 60% (add-on trial 50%; mono-therapy trial 81%). Levetiracetam was equally effective for partial and generalized seizures. Side effects were less common in the mono-therapy trial. Tiredness (7.8%) and aggressiveness (5%) were the most common side effects, and were dose-related, but were no reason to discontinue levetiracetam. In 25% of the children, a positive effect was seen on behaviour and/or alertness. This could not be related directly to seizure control. Overall, these two clinical trials confirm that levetiracetam is a broad spectrum anti-epileptic drug with a favourable safety profile. The positive effect on behaviour needs further quantitative study.
Subject(s)
Anticonvulsants/administration & dosage , Epilepsies, Partial/drug therapy , Epilepsy, Generalized/drug therapy , Piracetam/analogs & derivatives , Piracetam/administration & dosage , Adolescent , Anticonvulsants/adverse effects , Child , Child, Preschool , Cohort Studies , Dose-Response Relationship, Drug , Drug Administration Schedule , Drug Therapy, Combination , Electroencephalography/drug effects , Epilepsies, Myoclonic/drug therapy , Epilepsies, Partial/chemically induced , Epilepsy, Absence/drug therapy , Epilepsy, Generalized/chemically induced , Female , Humans , Infant , Levetiracetam , Male , Piracetam/adverse effects , Prospective Studies , Syndrome , Treatment OutcomeABSTRACT
Acute cerebellitis is an inflammatory process involving the cerebellum. We report the clinical, CT and MRI features of four cases and a review of the literature. Bilateral diffuse hemispheric abnormalities represent the most common imaging presentations. Our observations demonstrate the various imaging appearances of acute cerebellitis. Simultaneous involvement of both hemispheres and the vermis has not been reported previously. The development of cerebellar atrophy following an initial normal MR imaging examination is also a new finding. In atypical clinical presentation, MR imaging can lead to the diagnosis. MR imaging findings have, however, no prognostic value.
Subject(s)
Cerebellar Ataxia/diagnosis , Cerebellar Diseases/diagnosis , Cerebellum/pathology , Encephalitis/diagnosis , Magnetic Resonance Imaging/methods , Acute Disease , Adult , Anti-Bacterial Agents/therapeutic use , Anti-Inflammatory Agents/therapeutic use , Cerebellar Ataxia/drug therapy , Cerebellar Ataxia/etiology , Cerebellar Diseases/complications , Cerebellar Diseases/drug therapy , Child , Child, Preschool , Encephalitis/complications , Encephalitis/drug therapy , Female , Fever/etiology , Humans , Leukocyte Count , Prednisolone/therapeutic useABSTRACT
We present a patient with juvenile neuronal ceroid lipofuscinosis who developed a neuroleptic malignant syndrome when treated for hallucinations with a very low dose of risperidone, an atypical neuroleptic medication with usually few extrapyramidal side-effects. The loss of dopaminergic neurons in this condition may make these patients more vulnerable to this severe adverse effect.
Subject(s)
Antipsychotic Agents/adverse effects , Neuroleptic Malignant Syndrome/complications , Neuronal Ceroid-Lipofuscinoses/complications , Risperidone/adverse effects , Adult , Antipsychotic Agents/therapeutic use , Creatine Kinase/blood , Dopamine/physiology , Female , Fever/complications , Hallucinations/drug therapy , Humans , Intellectual Disability , Neuronal Ceroid-Lipofuscinoses/psychology , Risperidone/therapeutic useABSTRACT
In recent years, different research lines have examined the epileptogenic process in order to understand the different stages in this process, and with the hope that early recognition and intervention could prevent chronic epilepsy in patients with epileptic seizures. In animals, acquired epilepsy is studied most commonly with kindling models, status epilepticus models and traumatic brain injury models. Molecular genetic studies substantially help to understand age-specific channel and receptor abnormalities. Major progress has been made in recent years and we are now waiting for the first large scale multi-center clinical trials that test the possible anti-epileptogenic properties of anti-epileptic drugs or other compounds in well defined patient groups. In clinical practice, a structured diagnostic work-up in all patients with recurrent seizures is a first and necessary step in the recognition of patients at risk for developing chronic and refractory epilepsy.
Subject(s)
Epilepsy/etiology , Epilepsy/physiopathology , Animals , Anticonvulsants/pharmacology , Epilepsy/drug therapy , Humans , Kindling, Neurologic , Models, AnimalABSTRACT
In this open-label add-on study of levetiracetam in refractory childhood epilepsy syndromes, we studied the effect of a rapid introduction of levetiracetam on the total seizure frequency in 21 children, known to have partial and generalized seizures. Starting dosage was 10 mg/kg/day, increased every 4th day by 10 mg/kg up to a maximum of 60 mg/kg/day, depending on efficacy and tolerability. In this highly refractory population, 47% showed a seizure frequency reduction of more than 50%. Levetiracetam was effective both in partial and generalized seizures, with a significant effect on myoclonic seizures. Only mild side-effects were observed in four of 21 children, at a dosage of more than 40 mg/kg/day.
Subject(s)
Anticonvulsants/therapeutic use , Epilepsies, Partial/drug therapy , Epilepsy, Generalized/drug therapy , Piracetam/analogs & derivatives , Piracetam/therapeutic use , Adolescent , Anticonvulsants/administration & dosage , Child , Child, Preschool , Drug Administration Schedule , Female , Humans , Infant , Levetiracetam , Male , Piracetam/administration & dosage , SyndromeABSTRACT
The authors report 1-year prospective data on eight patients with Friedreich ataxia. Idebenone did not halt the progression of ataxia. At the end of therapy, cardiac ultrasound demonstrated significant reduction of cardiac hypertrophy in six of eight patients. Cardiac strain and strain rate imaging showed that the reduction of hypertrophy is preceded by an early and linear improvement in cardiac function. Idebenone reduced erythrocyte protoporphyrin IX levels in five of six patients with elevated baseline levels; however, changes did not consistently relate to cardiac improvement.
Subject(s)
Benzoquinones/therapeutic use , Free Radical Scavengers/therapeutic use , Friedreich Ataxia/complications , Hypertrophy, Left Ventricular/drug therapy , Adolescent , Adult , Benzoquinones/pharmacology , Cardiomyopathy, Hypertrophic/drug therapy , Cardiomyopathy, Hypertrophic/etiology , Cardiomyopathy, Hypertrophic/physiopathology , Child , Erythrocytes/chemistry , Female , Free Radical Scavengers/pharmacology , Free Radicals , Friedreich Ataxia/blood , Friedreich Ataxia/genetics , Friedreich Ataxia/physiopathology , Humans , Hypertrophy, Left Ventricular/etiology , Hypertrophy, Left Ventricular/physiopathology , Iron-Binding Proteins/genetics , Iron-Binding Proteins/physiology , Male , Mitochondria/metabolism , Oxidative Stress , Prospective Studies , Protoporphyrins/blood , Stroke Volume , Ubiquinone/analogs & derivatives , FrataxinABSTRACT
Desmoplastic infantile ganglioglioma is a rare intracranial tumor of early childhood with a usually excellent prognosis despite malignant features both radiologically and histologically. We present the case of a desmoplastic infantile ganglioglioma with histologically highly anaplastic features and both intracerebral and pial metastases. After partial resection the tumor was rapidly progressive and new metastases appeared. A combination of vincristine and carboplatinum was used according to the Low Grade Glioma Protocol of the International Society of Pediatric Oncology, with a temporary good response. When histologically characterized by highly anaplastic features, it seems the biologic behavior of this tumor remains uncertain. The aggressive behavior and the responsiveness to chemotherapy in this case may challenge the belief in the benign nature of these rare tumors.
Subject(s)
Brain Neoplasms/pathology , Ganglioglioma/secondary , Antineoplastic Combined Chemotherapy Protocols/therapeutic use , Biomarkers, Tumor/analysis , Brain/pathology , Brain Neoplasms/chemistry , Brain Neoplasms/therapy , Carboplatin/administration & dosage , Child, Preschool , Desmin , Fatal Outcome , Female , Ganglioglioma/chemistry , Ganglioglioma/therapy , Humans , Immunohistochemistry , Magnetic Resonance Imaging , Neoplasm Proteins/analysis , Vincristine/administration & dosageABSTRACT
This rare case of brain stem tethering presented with chronic and progressive geniculate neuralgia. In view of the fact that an occipital subcutaneous lipoma had been resected in childhood, it probably concerned a primary tethering, fitting in with an occult occipital dysraphism. Magnetic resonance imaging (MRI) clearly demonstrated an underlying tethering, causing a distortion of the brain stem. Consequently, this led to the hypothesis that the geniculate neuralgia could be explained by traction on the lower cranial nerves secondary to the brain stem displacement. Untethering resulted in a considerable decrease of the neuralgia. MRI proved to be essential in the diagnosis and treatment of this unusual case.
Subject(s)
Brain Stem/abnormalities , Facial Nerve Diseases/etiology , Adult , Brain Stem/pathology , Brain Stem/surgery , Facial Nerve Diseases/pathology , Facial Nerve Diseases/surgery , Female , Humans , Magnetic Resonance ImagingABSTRACT
PURPOSE: To improve patient compliance with and acceptance of intravesical oxybutynin therapy for neurogenic bladder dysfunction we developed a stable oxybutynin solution that eliminates the complicated crushing procedure. MATERIALS AND METHODS: From January 1995 to January 1997 we prospectively evaluated 15 children with a mean age of 6.1 years with persistent detrusor hyperactivity or significant side effects on oral oxybutynin therapy who received intravesically 0.2 mg./kg. (maximum 5 mg.) of a stable oxybutynin solution (5 mg./5 ml., pH 5.85) twice daily. RESULTS: The oxybutynin solution remained stable up to 24 months. In 13 of the 15 children therapeutic compliance was excellent. Detrusor hyperactivity decreased and systemic side effects were absent or minimal. After 4 and 24 months mean cystometric bladder capacity plus or minus standard error of mean increased from 114+/-15.2 to 161+/-26.6 and 214+/-21.7 ml. (p <0.01), mean ratio of cystometric-to-expected bladder capacity increased from 0.88+/-0.12 to 1.18+/-0.14 and 1.24+/-0.16 (p <0.01), and end filling bladder pressure decreased from 57.0+/-7.1 to 25.6+/-4.4 and 30.8+/-4.4 cm. water (p <0.01), respectively. CONCLUSIONS: Intravesical instillation of a specially prepared oxybutynin solution is safe and reliable in children with persistent detrusor hyperactivity or side effects on oral oxybutynin therapy. Eliminating the complex crushing preparation of the solution by the child or parent has made this therapy easy to use and acceptable in the long term.
Subject(s)
Cholinergic Antagonists/administration & dosage , Mandelic Acids/administration & dosage , Patient Compliance , Urinary Bladder, Neurogenic/drug therapy , Administration, Intravesical , Adolescent , Child , Child, Preschool , Drug Stability , Female , Humans , Infant , Male , Prospective Studies , SolutionsABSTRACT
PURPOSE: To unravel why intravesical oxybutynin is more effective and causes significantly fewer systemic side effects than oral oxybutynin in the treatment of neurogenic bladder dysfunction, we tested the hypothesis that the absorption and metabolism of oxybutynin are changed after intravesical instillation. MATERIALS AND METHODS: A high-performance liquid chromatography assay was developed for both oxybutynin and its active metabolite, N-desethyl-oxybutynin. Plasma concentrations were quantified after intravesical (n = 11) and oral (n = 5) administration of oxybutynin in children under steady-state conditions. Pharmacokinetic parameters were calculated. RESULTS: Oral administration of oxybutynin (0.2 mg./kg./dose) resulted in peak plasma concentrations for N-desethyl-oxybutynin which were 7.4 +/- 1.3 times higher than corresponding values for oxybutynin (n = 5). Also the AUC (area under the plasma concentration time curve) values were higher for N-desethyl-oxybutynin compared with those of oxybutynin, the ratio being 10.8 +/- 1.0 (n = 5). Intravesical instillation (0.2 mg./kg./dose), on the other hand, resulted in reduced metabolite generation and peak plasma concentrations for N-desethyl-oxybutynin which were in the same range as those for oxybutynin, the ratio being 1.2 +/- 0.1 (n = 11). The ratio for the AUC values for N-desethyl-oxybutynin and oxybutynin was 2.1 +/- 0.2 (n = 11). CONCLUSIONS: The significantly lower AUC ratio of the N-desethyl metabolite over the mother compound, due to a reduced first pass metabolism, may explain the clinically relevant reduction of side effects that characterizes intravesical compared with oral oxybutynin therapy.
Subject(s)
Cholinergic Antagonists/administration & dosage , Mandelic Acids/administration & dosage , Urinary Bladder, Neurogenic/drug therapy , Administration, Intravesical , Administration, Oral , Adolescent , Child , Child, Preschool , Cholinergic Antagonists/adverse effects , Cholinergic Antagonists/metabolism , Female , Humans , Male , Mandelic Acids/adverse effects , Mandelic Acids/metabolismABSTRACT
It has been proposed that the pI(Cln) protein forms a nucleotide-sensitive plasma membrane anion channel with a GXGXG motif being an essential component of the extracellular nucleotide-binding site. To evaluate this hypothesis, we have performed voltage-clamp experiments on Xenopus laevis oocytes injected with RNA encoding a rat mutant pI(Cln) in which the three glycines of the putative nucleotide-binding site have been changed into alanines (G54A; G56A; G58A). The injected oocytes displayed outwardly rectifying anion currents, which were voltage-dependently blocked by extracellular cAMP, but which were not affected by removal of extracellular Ca2+. Furthermore, the mutation did not affect the voltage-dependent inactivation. We therefore conclude that there is no evidence in favour of an extracellular nucleotide-binding site in pI(Cln).
Subject(s)
Chloride Channels/chemistry , Ion Channels , Animals , Calcium/physiology , Chloride Channels/physiology , Cyclic AMP/pharmacology , Electric Conductivity , Extracellular Space , Ion Channel Gating , Membrane Potentials , Mutagenesis, Site-Directed , Oocytes , Rats , Structure-Activity Relationship , Xenopus Proteins , Xenopus laevisABSTRACT
Chloride channel protein (ClC)-6a and ClC-6c, a kidney-specific splice variant with a truncated C-terminus, are proteins that belong structurally to the family of voltage-dependent chloride channels. Attempts to characterize functionally ClC-6a or ClC-6c in Xenopus oocytes have so far been negative. Similarly, expression of both ClC-6 isoforms in mammalian cells failed to provide functional information. One possible explanation of these negative results is that ClC-6 is an intracellular chloride channel rather than being located in the plasma membrane. We therefore studied the subcellular location of ClC-6 isoforms by transiently transfecting COS and CHO cells with epitope-tagged versions of ClC-6a and ClC-6c. Confocal imaging of transfected cells revealed for both ClC-6 isoforms an intracellular distribution pattern that clearly differed from the peripheral location of CD2, a plasma-membrane glycoprotein. Furthermore, dual-labelling experiments of COS cells co-transfected with ClC-6a or -6c and the sarco/endoplasmic-reticulum Ca2+ pump (SERCA2b) indicated that the ClC-6 isoforms co-localized with the SERCA2b Ca2+ pump. Thus ClC-6a and ClC-6c are intracellular membrane proteins, most likely residing in the endoplasmic reticulum. In view of their structural similarity to proven chloride channels, ClC-6 isoforms are molecular candidates for intracellular chloride channels.
Subject(s)
Calcium-Transporting ATPases/metabolism , Chloride Channels/metabolism , Endoplasmic Reticulum/enzymology , Sarcoplasmic Reticulum/enzymology , Animals , CHO Cells , COS Cells , Cricetinae , Fluorescent Antibody Technique, Indirect , Microscopy, Confocal , Oocytes/metabolism , Transfection , XenopusABSTRACT
ClC-6 is a protein that structurally belongs to the family of ClC-type chloride channels. We now report the identification of three additional ClC-6 isoforms that are truncated because of alternative splicing. We have isolated, from human K562 cells, four types of ClC-6 cDNAs that encode four distinct ClC-6 protein isoforms. ClC-6a (869 amino acids) corresponds to the previously published ClC-6 protein [Brandt and Jentsch (1995) FEBS Lett. 377, 15-20] and it has a canonical ClC structure. However, ClC-6b (320 amino acids), ClC-6c (353 amino acids) and ClC-6d (308 amino acids) are truncated at their C-termini. Hydropathy-plot analysis indicates that the shortened isoforms contain maximally four (ClC-6b and -6d) or seven (ClC-6c) transmembrane domains. Sequence analysis of a human genomic ClC-6 fragment indicates that the cDNA variability arises from alternative splicing at two different positions: the first alternative site consists of an intron flanked by two alternative donor sites and two alternative acceptor sites, the second being due to an exon that is optionally included or excluded. Reverse-transcription-PCR analysis of ClC-6 expression in human cell lines and tissues shows that the majority (83%) of ClC-6 mRNAs consists of ClC-6a or ClC-6c messengers. Furthermore, in a mouse tissue panel, the ClC-6a mRNA has a relatively broad tissue expression pattern, since it could be detected in brain, kidney, testis, skeletal muscle, thymus and pancreas. In contrast, expression of ClC-6c is more restricted, since it was only detected in kidney.
Subject(s)
Alternative Splicing , Chloride Channels/biosynthesis , Kidney/metabolism , Transcription, Genetic , Amino Acid Sequence , Animals , Base Sequence , Cell Line , Chloride Channels/chemistry , Fetus , HeLa Cells , Humans , Jurkat Cells , KB Cells , Male , Mice , Molecular Sequence Data , Polymerase Chain Reaction , RNA, Messenger/biosynthesis , Sequence Alignment , Tumor Cells, CulturedABSTRACT
pICln is a protein that induces an outwardly rectifying, nucleotide-sensitive chloride current (ICln) when expressed in Xenopus oocytes, but its precise function (plasma-membrane anion channel versus cytosolic regulator of a channel) remains controversial. We now report that a chloride current identical to ICln is induced when Xenopus oocytes are injected with human ClC-6 RNA. Indeed, both the pICln and the ClC-6 induced current are outwardly rectifying, they inactivate slowly at positive potentials and have an anion permeability sequence NO3- > I- > Br- > Cl- > gluconate. Cyclamate, NPPB, and extracellular cAMP block the induced currents. The success rate of current expression is significantly increased when the injected Xenopus oocytes are incubated at a higher temperature (24 or 37 degrees C) prior to the analysis. In addition, the ICln current was detected in 6.2% of noninjected control Xenopus oocytes. We therefore conclude that the ICln current in Xenopus oocytes corresponds to an endogenous conductance that can be activated by expression of structurally unrelated proteins. Furthermore, functional, biochemical, and morphological observations did not support the notion that pICln resides in the plasma membrane either permanently or transiently after cell swelling. Thus, it is unlikely that pICln forms the channel that is responsible for the ICln current in Xenopus oocytes.