Study on the occurrence of microorganisms on the post-surgical maxillary prostheses with obturators and in the post-surgical cavities of maxilla.

The bacterial environment of the mouth cavity may be subjected to change under influence of various factors, such as surgical removal of neoplasm tumors and in consequence the wearing of post-surgical prostheses with obturators. The purpose of the paper was to study the conceivable differences in occurrence of particular types of microorganisms found on the margin of post-surgical cavities and on the prosthetic obturators. The performed microbiologic examinations revealed that more pathologic bacterial flora was found on the obturators than in the post-surgical cavities. The authors conclude that the post-surgical patients should pay more attention to the very accurate hygiene of their prostheses and the mouth cavity as well.

[Chlamydia trachomatis and concomitant flora in the internal genital organs of women without clinical symptoms of infection]. / Chlamydia trachomatis i flora wspólistniejaca w wewnetrznych narzadach plciowych kobiet bez klinicznych objawów zapalnych.

One thousand and forty swabs from 52 gynaecological patients were examined bacteriologically. The most frequently isolated pathogen was C. trachomatis found in the endocervix in 19.2%, in the endometrium in 17.3% and in the fallopian tube in 48.1% of the examined women. There was also shown that presence of C. trachomatis in utero and in fallopian tube not always coexist with it's presence in the endocervix, move over presence of that microbe doesn't have influence on presence of any bacteria in genitourinary tract.

[Epidemiologic investigation of infection with Klebsiella using phage typing as a method]. / Epidemiologiczne badania zakazen paleczkami Klebsiella z uzyciem lizotypii jako metody.

In epidemiological investigations of infections with Klebsiella carried on by us for many years, a lysotyping method was used with a success. The results which are now being presented deal with 638 strains of Klebsiella pneumoniae and Klebsiella oxytoca originating from patients treated in hospitals in Wroclaw, Kraków, Gdansk and Sosnowiec during the years 1986-1992. Susceptibility of strains to the standard set of 14 phages used since ten years decreased significantly, when compared with previously typed strains of Klebsiella. Out of 312 susceptible strains of Klebsiella, majority (302) represented 47 phage types described previously. Sporadic appearance of new phage types was observed. Some phagotypes (37, 47, 112, 117, 119, 142, 153 and 157) were represented most frequently and concerned 55% of susceptible strains. High repeatability of phage types in consecutive groups of typed strains suggests a need for continuation of this method for typing Klebsiella. Decreased susceptibility of strains to standard set of phages requires introduction of new typing bacteriophages.

[Usefulness of plasmids as epidemiologic markers for typing of Klebsiella]. / Przydatnosc plazmidów jako epidemiologicznych markerów do typowania paleczek Klebsiella.

The study was aimed at evaluation of utility of plasmids as epidemiological markers for interspecific differentiation of 200 strains of Klebsiella isolated from patients with generalized infections and hospitalized at intensive care ward of the Gdansk Medical Academy. Strains isolated from the ward environment were also investigated. In the first phase of this study classical methods of typing were used, such as determination of the biochemical, bacteriocin (susceptibility to 8 bacteriocins) and phage types, and MIC for 9 antibiotics. Basing on these methods it was found that 41 types of Klebsiella pneumoniae, 13 types of Klebsiella oxytoca and 1 type of Klebsiella ozenae occur. Six groups of bacteria were differentiated in the group which had the same type. These strains were investigated for plasmid and restrictive patterns. Identity of types occurring in individual groups was detected. For one of them, a restrictive pattern was determined by cleavage of plasmid DNA by a restrictive Eco RV enzyme. In remaining groups such pattern was not found, both after treatment with Eco RV and Hha I enzyme and this may be associated with modification of the plasmid DNA. Strains belonging to one of these groups did not contain plasmids in their cells.

Phage typing of Klebsiella strains in epidemiological studies.

A set of 14 routinely applied bacteriophages was used for the typing of Klebsiella pneumoniae and Klebsiella oxytoca strains isolated from patients in 1983-1986. The phage-sensitive strains (65%) differed in respect to the place and time of isolation which manifested itself by an occurrence of specific lysotypes in Wroclaw, Gdansk, Wernigerode and Wloclawek, as well as various repeatibility on the lysotypes within 10 years.

Phage-typing of Klebsiella strains from Cologne and Wroclaw.

276 strain of Klebsiella pneumoniae and Klebsiella oxytoca were phage-typed. 126 of these strains were isolated from clinical sources in the Institute of Hygiene in Cologne and 150 came from Wroclaw. The set of 19 phages used for phage-typing was chosen out of 46 Klebsiella specific bacteriophages. 80% of the isolates from Cologne were sensitive to the phages in comparison to 83% of the strains from the Wroclaw region. The phage sensitivity of the German strain differed from that of the Polish strains. 28 of the 67 phage-types were seen in Cologne and 35 in Wroclaw, only 6 phage-types were observed in both regions. The frequency and the spectrum of the phage-types were different, too. Phage-types denoted by the numbers 1, 17, 32, 36, 37, 59, 64 appeared to be most frequent (10 to 16 strains). No significant differences in the sensitivity to typing phages between strains of Klebsiella pneumoniae and Klebsiella oxytoca were demonstrated.