[The epidemic situation of tick-borne encephalitis and Lyme disease in the city of Tomsk]. / Epidemicheskaia situatsiia po kleshchevomu éntsefalitu i bolezni Laima v g. Tomske.

Lyme disease and tick-borne encephalitis have similar principal epidemiological features chiefly associated with their basic carrier and determinants of human infection. The cases of Lyme disease, as evidenced by laboratory studies, indicate that there are active natural foci of this infection in the city, frequently combining with natural foci of tick-borne encephalitis. At the same time a great quantity of cases of tick-borne encephalitis and Lyme disease (mixed infection) is notified in Tomsk.

[Cloning fragments of the RNA polymerase gene of an attenuated variant of the foot-and-mouth disease virus A22]. / Klonirovanie fragmentov gena RNK-polimerazy attenuirovannogo varianta virusa iashchura A22.

The cDNA fragments complementary to RNA-polymerase gene and 3'-untranslated genome region of attenuated foot-and-mouth disease virus strain A(22)645 have been synthesized and cloned into a plasmid vector pUC19 in E. coli JM109. The cloned cDNA fragments were characterized as to their size, orientation towards the plasmid, and localization in the virus genome. Restriction maps for complete gene and two cDNA clones were constructed.

[Primary structure of the 3'-terminal region of the RNA-polymerase gene in foot-and-mouth virus A22]. / Pervichnaia struktura 3'-kontsevoi oblasti gena RNK-polimerazy virusa iashchura A22.

This study was undertaken for the purpose of determining the primary structure of the 3' end gene of RNA polymerase of foot-and-mouth disease virus A22 550. Reported are isolation and purification of the virus, isolation of RNA, synthesis of cDNA, experience obtained from cloning as well as analysis of hybridisation and isolation of plasmid DNA. Nucleotide sequences, characterised by specific clones, were tested for potential needle structures. Also described are homology comparisons among FMD virus types A12, A10, O1, and C1.