ABSTRACT
The role of oxidative/reductive balance derangement in the pathogenesis of diabetic microangiopathy has often been discussed in the last few years. Therefore, we decided to evaluate the influence of intensive insulin therapy on selected indicators of free radical production. The levels of plasma hydrogen peroxide (H2O2) and serum malonyldialdehyde (MDA) were estimated in 15 patients with Type 1 and 15 with Type 2 diabetes before and after 2 weeks of intensive treatment. The initial H2O2 and MDA levels in all cases were significantly higher than in controls. After 2 weeks of treatment, the values for both estimated parameters were significantly lower; however, they were still higher than in the control group. Our results seem to confirm the previous suggestions concerning the relation between metabolic disturbances and oxidative stress in diabetic patients.
Subject(s)
Diabetes Mellitus, Type 1/blood , Diabetes Mellitus, Type 1/drug therapy , Diabetes Mellitus, Type 2/blood , Diabetes Mellitus, Type 2/drug therapy , Hydrogen Peroxide/blood , Insulin/therapeutic use , Malondialdehyde/blood , Adolescent , Adult , Aged , Biomarkers/blood , Blood Glucose/metabolism , Diabetic Angiopathies/physiopathology , Female , Free Radicals , Fructosamine , Hexosamines/blood , Humans , Male , Middle Aged , Oxidation-Reduction , Reference Values , Time FactorsABSTRACT
It was established that metabolic disturbances accompanying the impairment of diabetes control induced polymorphonuclear neutrophil (PMN) activation. Therefore, we evaluated the influence of diabetes control, measured as HbA1, level on superoxide anions production and neutrophils adherence to foreign surface. Twenty patients with insulin-dependent or non-insulin-dependent diabetes being in different state of metabolic control were studied. It was revealed that PMN from diabetics demonstrated significantly increased unstimulated superoxide anion production (11.07 +/- 1.25 vs. 4.15 +/- 1.24 nmol O2-/10(6) PMN/10 min). Stimulated O2- production did not reach the same levels as controls (21.80 +/- 1.34 vs. 23.67 +/- 4.36 nmol O2-/10(6) PMN/10 min). There were highly significant correlations between unstimulated O2- production and HbA1, levels (r = 0.86). Adherence of diabetic PMN suspended in autologous plasma was significantly increased in comparison to controls (12.76 +/- 1.20 vs. 6.04 +/- 1.99 cells/mm2). However, these data did not correlate with HbA1 level (r = 0.06). In conclusion our results seems to indicate that metabolic control of diabetes influence PMN function.
