ABSTRACT
The identification of Brucella can be a time-consuming and labor-intensive process that places personnel at risk for laboratory-acquired infection. Here, we describe a real-time PCR assay for confirmation of presumptive Brucella isolates. The assay was designed in a multiplex format that will allow the rapid identification of Brucella spp., B. abortus, and B. melitensis in a single test.
Subject(s)
Brucella abortus/isolation & purification , Brucella melitensis/isolation & purification , Brucella/isolation & purification , Polymerase Chain Reaction/methods , Base Sequence , Brucella/classification , Brucella/genetics , Brucella abortus/classification , Brucella abortus/genetics , Brucella melitensis/classification , Brucella melitensis/genetics , Computer Systems , DNA Primers , RNA, Bacterial/genetics , RNA, Ribosomal, 16S/geneticsABSTRACT
The incidence of serogroup Y meningococcal disease has increased recently in the United States. Here, we describe the development of a 5' exonuclease assay for the detection of serogroup Y Neisseria meningitidis and demonstrate the usefulness of this assay for resolving serogroup identification of strains that are resistant to conventional serogrouping and for the nonculture identification of serogroup Y meningococcal disease.
