Subject(s)
Brain-Derived Neurotrophic Factor/biosynthesis , Coronary Vessels/pathology , Macrophages/metabolism , Myocardial Infarction/metabolism , Myocardium/pathology , Neovascularization, Pathologic/metabolism , Animals , Coronary Vessels/metabolism , Disease Models, Animal , Endothelium, Vascular/metabolism , Endothelium, Vascular/pathology , Immunohistochemistry , Male , Myocardial Infarction/pathology , Myocardium/metabolism , Rats , Rats, Sprague-DawleyABSTRACT
Advanced glycation end products (AGEs) are associated with the pathogenesis of various diseases. AGEs induce excess accumulation of extracellular matrix and expression of profibrotic cytokines. In addition, studies on receptor for advanced glycation end products (RAGE) have shown that the ligand-RAGE interaction activates several intracellular signaling cascades associated with several fibrotic diseases. We investigated the expression of AGEs and RAGE in samples from patients with idiopathic pulmonary fibrosis (IPF) and non-specific interstitial pneumonia (NSIP). Lung tissues and plasma samples from patients with IPF (n=10), NSIP (n=10), and control subjects (n=10) were obtained. Expression of AGEs and RAGE was determined by immunofluorescence assay of lung tissue. Circulating AGEs were measured by Western blot and enzyme-linked immunosorbent assay. Lungs with IPF showed strong expression for both AGEs and RAGE compared to that in NSIP and controls. However, no difference in AGE or RAGE expression was observed in lungs with NSIP compared to that in the controls. Levels of circulating AGEs also increased significantly in lungs of patients with IPF compared to those with NSIP and normal control. Increased AGE-RAGE interaction may play an important role in the pathogenesis of IPF.
Subject(s)
Glycation End Products, Advanced/metabolism , Idiopathic Pulmonary Fibrosis/metabolism , Lung Diseases, Interstitial/metabolism , Receptors, Immunologic/biosynthesis , Aged , Blotting, Western , Enzyme-Linked Immunosorbent Assay , Female , Fluorescent Antibody Technique , Glycation End Products, Advanced/analysis , Humans , Male , Middle Aged , Receptor for Advanced Glycation End Products , Receptors, Immunologic/analysisABSTRACT
Leptin regulates energy balance. However, knowledge of the critical intracellular transducers of leptin signaling remains incomplete. We found that Rho-kinase 1 (ROCK1) regulates leptin action on body weight homeostasis by activating JAK2, an initial trigger of leptin receptor signaling. Leptin promoted the physical interaction of JAK2 and ROCK1, thereby increasing phosphorylation of JAK2 and downstream activation of Stat3 and FOXO1. Mice lacking ROCK1 in either pro-opiomelanocortin (POMC) or agouti-related protein neurons, mediators of leptin action, displayed obesity and impaired leptin sensitivity. In addition, deletion of ROCK1 in the arcuate nucleus markedly enhanced food intake, resulting in severe obesity. Notably, ROCK1 was a specific mediator of leptin, but not insulin, regulation of POMC neuronal activity. Our data identify ROCK1 as a key regulator of leptin action on energy homeostasis.
Subject(s)
Energy Metabolism/physiology , Hypothalamus/metabolism , Leptin/physiology , Receptors, Leptin/physiology , rho-Associated Kinases/physiology , Action Potentials/genetics , Action Potentials/physiology , Agouti-Related Protein/physiology , Animals , Appetite Regulation/genetics , Appetite Regulation/physiology , Arcuate Nucleus of Hypothalamus/metabolism , Cells, Cultured , Eating , Janus Kinase 2/metabolism , Leptin/pharmacology , Mice , Mice, Inbred C57BL , Mice, Mutant Strains , Mice, Transgenic , Neurons/metabolism , Obesity/genetics , Phosphorylation , Pro-Opiomelanocortin/metabolism , Receptors, Leptin/agonists , Receptors, Leptin/antagonists & inhibitors , STAT3 Transcription Factor/metabolism , rho-Associated Kinases/geneticsABSTRACT
For quantitative analysis of nanoparticle diffusions and submicro-environments in living cells, use of newly synthesized silica-based fluorescent nanoparticle (Si-FNP) as a standard nanoprobe is successfully demonstrated. The appropriate characteristics of a standard probe were fully analyzed in vitro by single molecule detection, transmission electron microscopy, and dynamic light scattering. Using fluorescence correlation analysis in single living cells, we quantitatively compared the diffusional properties of the standard Si-FNP with a diameter of 50 nm, peptide coated Si-FNP, streptavidin coated Qdot, and GFP molecule which have different sizes and surface properties. The result demonstrates that the standard Si-FNP without coat is minimally trapped in the vesicles in the process of cellular endocytosis. Interestingly, a large proportion of Si-FNP introduced into the cells by electroporation diffuses freely in the cells during a cell cycle suggesting free diffusing NPs are hardly trapped in the vesicles. The simple but highly sensitive method will provide insight into strategies to understanding the hydrodynamic process of nanoparticle delivery into living cells as well as the cellular microenvironment in the view of submicro-size.
Subject(s)
Nanoparticles/chemistry , Silicon Dioxide/chemistry , Silicon Dioxide/metabolism , Cell Line, Tumor , Electroporation , Endocytosis , Fluorescein-5-isothiocyanate/chemistry , Fluorescence , Fluorescent Dyes/chemistry , Green Fluorescent Proteins/metabolism , Humans , Peptide Fragments/chemistry , Quantum Dots , Rhodamines/chemistry , Streptavidin/chemistry , tat Gene Products, Human Immunodeficiency Virus/chemistryABSTRACT
Epilepsy constitutes a significant public health problem, and even the newest drugs and neurosurgical techniques have proven unable to cure the disease. In order to select a group of isolates which could generate an active compound with neuroprotective or antiepileptic properties, we isolated 517 actinomycete strains from soil samples taken from Jeju Island, in South Korea. We then screened these strains for possible anti-apoptotic effects against serum deprivation-induced hippocampal cell death, using the 3-(4, 5-dimethylthiazol-2-yl)2,5-diphenyl-tetrazolium bromide (MTT) assay as an in vitro test. The excitotoxic glutamate analog, kainic acid (KA), was used to induce seizures in experimental mice in our in vivo tests. As a result of this testing, we located one strain which exhibited profound neuroprotective activity. This strain was identified as a Streptomyces species, and exhibited the rifampin-resistant genotype, Asn(AAC)442, according to the results of 16S rRNA and rpoB gene analyses.
