ABSTRACT
White lupin seed is a unique legume rich in protein and fiber contents, as well as phytochemicals with health potential that contributes to a reduced risk of dyslipidemia, obesity and intestinal dysfunction. This study was focused on the effect of the year on the contents of caffeic acid, 4-hydroxybenzoic acid, trans-ferulic, trans-p-coumaric, quercetin, myricetin, kaempferol, apigenin and genistein, as well as the antioxidant activity and total polyphenols, of seeds of eleven varieties (Lupine albus). The contents of individual phenolic substances were determined by high-performance liquid chromatography-HPLC. The total content of polyphenols and the antioxidant activity were determined spectrophotometrically. The results show that the lowest contents of phenolic acids were found in the seeds from 2018. The caffeic acid and trans-ferulic acid were the most represented among all phenolic acids, during all 3 monitored years (2017, 2018, and 2019). Our results confirm the significant influence of the year of cultivation on the bioactive substances' content in the seeds, and this can be potentially useful for the appropriate selection of locations for lupine growers in Slovakia, taking into account the climatic conditions of the given location. This study provides information about a legume that is underutilized in human nutrition, which may be a valuable source of bioactive substances.
ABSTRACT
Walnuts (fruits Juglans regia, L.) belong among to the most spread, commercially grown tree nuts in the world. Nuts have grown higher attention in last years as valuable sources of essential elements; they are rich in non-essential fatty acids, proteins, fibre, plant sterols and minerals including potassium and magnesium and antioxidants. Determination of elements distribution in nuts is required not only for evaluation of total intake of essential elements in our food, but it is also useful for gaining data on contamination of foodstuffs by risk elements, but also for monitoring of heavy metal contamination in foodstuffs. Walnut samples were taken from three selected areas in Slovakia. The first location is in the vicinity of the cities Strázske, Humenné and Vranov nad Toplou, the second one is situated close to the city of Prievidza and the last one is close to the city of Vráble. The results of evaluation showed that the content of the elements monitored in location of Vráble did not exceed the limits set by the Regulation of Ministry of Health of Slovak Republic No 2/1994. In the half of samples taken from the location of Strázske, higher content of cadmium as the permitted limit has been found. The samples exceeded the amounts allowed by the Regulation No 2/1994 in contaminated area called "The dangerous triangle". Potential consumption of these walnuts could negatively affect human organism, but only if the kernels from the exact locality would be consumed in very high daily amounts (100-fold higher intake as daily intake according to the Statistical Office of SR).
