ABSTRACT
Introducción: La migración intrínsicamente implica la necesidad de adaptación a nuevos estilos de vida, siendo más radical cuando se trata de países tan lejanos y distintos, como India o China. Las variantes electrocardiográficas en estas culturas están siendo ampliamente estudiadas para entender cuando estos cambios son una anomalía o una adaptación fisiológica normal. Por este motivo se hace imperativo investigar sobre hallazgos electrocardiográficos de una población en constante aumento en nuestra sociedad. Objetivo: Evaluar los hallazgos electrocardiográficos en 2 poblaciones de inmigrantes asiáticos con más de 2 años de residencia en Iquique, Chile. Metodología: Estudio analítico, descriptivo de corte transversal. Análisis de electrocardiograma (ECG) de 12 derivaciones, frecuencia cardíaca, intervalos PR, QTc y conclusiones diagnósticas del trazado. Resultados: El grupo Chino (CHI) de 43 pacientes (37,2 por ciento mujeres), edad de 39,5+13 años. Grupo Indio (IND) de 41 pacientes (48,8 por ciento mujeres) con edad promedio de 38,6+10 años. CHI se encontró un 79,1 por ciento de trazadosalterados con 55,8 por ciento Bloqueo incompleto de Rama Derecha (BIRD), 11,6 por ciento que mostraba signos de Bloqueo completo de Rama Derecha (BCRD), y un 11,6 por ciento presento un QTc alargado. IND el 75,6 por ciento de los pacientes tenía un ECG dentro de los límites normales, los ECG alterados mostraban a un 14,6 por ciento con BIRD y la pesquisa de QTc alargado fue de un 2 por ciento de la población. Conclusión: Existe un alto porcentaje de pacientes chinos con electrocardiogramas alterados y especialmente con bloqueos de rama derecha, lo que hace necesario estudiar las causas y el rol que tendría la occidentalización y las anomalías genéticas en esto.
Background: Immigration implies the adaptation to new life styles and these changes are more complicated when countries are far way as is the case between Chile and both India an China. It is uncertain whether the electrocardiographic differences between these populations reflect adaptation changes or are just differences in normal patterns for each population. Aim: to analyze characteristics of the electrocardiogram in subjects of Indian or Chinese descent living more than 2 years in Iquique, Chile. Method: We analyzed the heart rate (HR), PR interval, and QTc in 12 lead EKGs from subjects of Indian orChinese descent. Results: 43 subjects (37.2 percent women), aged 39.5±13 years were included in the Chinese group (CHI); 41 subjects (48.8 percent women), aged 38.6±10 years were included in the Indian group (IND). 79.1 percent of EKGs in the CHI group were abnormal: incomplete right bundle branch block (RBBB) was present in 55.8 percent 11.6 percent had complete RBBB and 11.6 percent a prolonged QT. On the other hand 75.6 percent of subjects in the IND group had a normal EKG; abnormalities included incomplete RBBB in 14.6 percent and prolonged QT in 2 percent. Conclusion: A high percentage of subjects of Chinese descent living for more than 2 years in Iquique, Chile, exhibit electrocardiographic abnormalities, mainly RBBB. Further study is needed to eucidate whether genetic differences or adaptation to new conditions underlie these characteristics.
Subject(s)
Humans , Male , Adult , Female , Middle Aged , Electrocardiography , Heart Rate/physiology , Asian People , Chile , China , Cross-Sectional Studies , India , Emigration and ImmigrationABSTRACT
We describe here a fatty acid-binding protein (FABP) isolated and purified from the parasitic protozoon Giardia lamblia. The protein has a molecular mass of 8 kDa and an isoelectric point of 4.96. A Scatchard analysis of the data at equilibrium revealed a dissociation constant of 3.12 x 10(-8) M when the labeled oleic acid was displaced by a 10-fold greater concentration of unlabeled oleic acid. Testosterone, sodium desoxycholate, taurocholate, metronidazol, and alpha-tocopherol, together with butyric, arachidonic, palmitic, retinoic, and glycocholic acids, were also bound to the protein. Assays with polyclonal antibodies revealed that the protein is located in the ventral disk and also appears in the dorsal membrane, the cytoplasm, and in the vicinity of the lipid vacuoles.
Subject(s)
Carrier Proteins/analysis , Giardia lamblia/chemistry , Protozoan Proteins/analysis , Animals , Carrier Proteins/chemistry , Carrier Proteins/isolation & purification , Carrier Proteins/metabolism , Chromatography, Affinity , Electrophoresis, Polyacrylamide Gel , Fatty Acid-Binding Proteins , Fluorescent Antibody Technique, Indirect , Giardia lamblia/metabolism , Giardia lamblia/ultrastructure , Immunohistochemistry , Immunoprecipitation , Isoelectric Focusing , Isoelectric Point , Microscopy, Immunoelectron , Molecular Weight , Protozoan Proteins/chemistry , Protozoan Proteins/isolation & purification , Protozoan Proteins/metabolism , Spectrometry, Fluorescence , Spectrometry, Mass, Matrix-Assisted Laser Desorption-IonizationABSTRACT
The secretory immune response in humans infected with Giardia lamblia was studied by using saliva samples and an 8-kDa antigen capable of binding fatty acids. This antigen was not recognized by saliva samples from healthy individuals. The antigen may be useful in diagnostic studies of G. lamblia infection.
Subject(s)
Antibodies, Protozoan/analysis , Antigens, Protozoan/immunology , Carrier Proteins/immunology , Giardia lamblia/immunology , Immunoglobulin A, Secretory/analysis , Neoplasm Proteins , Tumor Suppressor Proteins , Animals , Enzyme-Linked Immunosorbent Assay , Fatty Acid-Binding Protein 7 , Fatty Acid-Binding Proteins , Humans , Molecular Weight , Saliva/immunologyABSTRACT
Six trypanosomatids isolated from different geographical areas from South America (Peru and Brazil) and different vectors and reservoir hosts (the triatomine Panstrongylus chinai [TP1], Triatoma infestans [TP2], Rhodnius ecuadorensis [TP3], R. prolixus [TB1], Didelphys marsupialis [TB2]), and one from a human asymptomatic patient [TB3], were characterized using lectin agglutination, isoenzyme profile, in vitro culture final metabolite patterns, and compared with a reference strain (Trypanosoma cruzi, Maracay strain [TC]). The different isolates were cultured in vitro in Grace's medium supplemented with 10% inactivated bovine foetal serum. According to our results and the statistical study, the isolate obtained from R. ecuadorensis should be designed as a Trypanosoma rangeli sp., showing all other isolates strong similarities to T. cruzi. Between them, two clusters could be identified, strongly correlating with the geographical origin. Cluster I grouped isolates from Peru and T. cruzi reference strain, and cluster II grouped the three Brazilian isolates.
Subject(s)
Trypanosoma cruzi/growth & development , Trypanosoma/growth & development , Agglutination Tests , Animals , Brazil , Cluster Analysis , Disease Reservoirs , Humans , Insect Vectors , Isoelectric Focusing , Isoenzymes/analysis , Magnetic Resonance Spectroscopy , Opossums , Peru , Triatominae , Trypanosoma/chemistry , Trypanosoma/classification , Trypanosoma/enzymology , Trypanosoma cruzi/chemistry , Trypanosoma cruzi/classification , Trypanosoma cruzi/enzymologyABSTRACT
Se realizó un estudio abierto, no controlado, multicéntrico, realizado con intención de tratar, en 60 pacientes con dorsolumbalgia mecánica, en el cual evaluó la efectividad y seguridad de nimesulide 100 mg dos veces al día, administrado durante 15 días. El tratamiento produjo un alivio significativo en los escores de dolor entre el día de inicio del tratamiento y los 5 días, con un alivio adicional importante entre los días 5 y 10, sin alivios adicionales para el día 15. El tiempo medio para la desaparición total del dolor fue de 8,5 días. La limitación funcional fue evaluada el día 0 y el día 15, obteniéndose una mejoría significativa desde el punto de vista estadístico. Se reportaron efectos adversos en 4 de los pacientes, de los cuales sólo uno ameritó la suspensión del tratamiento
